Magnetic nano particle enzyme immobilization as well as preparation method and uses thereof

A technology of magnetic nanoparticles and hydrolase, applied in the direction of immobilization on or in inorganic carriers, fermentation, etc., to achieve the effects of easy preparation, simple handling and high enzymatic activity

Inactive Publication Date: 2008-05-14
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But this method of functionalizing magnetic nanoparticles has not been used for the immobilization of biomacromolecules

Method used

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  • Magnetic nano particle enzyme immobilization as well as preparation method and uses thereof
  • Magnetic nano particle enzyme immobilization as well as preparation method and uses thereof
  • Magnetic nano particle enzyme immobilization as well as preparation method and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 0.56g FeCl 3 ·6H 2 O and 0.2 g FeCl 2 4H 2 O was dissolved in 10ml of degassed secondary water, and the temperature was raised to 80°C under the protection of argon. Under vigorous mechanical stirring, 5ml of ammonia water with a concentration of 28% was quickly injected and kept for 20min. Cool down to room temperature, wash to neutral with deionized water, add 30mg 3,4 dihydroxybenzaldehyde, ultrasonic 30min, obtain aldehyde group functionalized magnetic nanoparticles A, the average particle diameter is 10nm (see figure 1 ).

Embodiment 2

[0059] 0.56g FeCl 3 ·6H 2 O and 0.2 g FeCl 2 4H 2 O was dissolved in 10ml of degassed deionized water, and the temperature was raised to 80°C under the protection of argon. Under vigorous mechanical stirring, 5ml of ammonia water with a concentration of 28% was quickly injected and kept for 20min. Cool down to room temperature, wash with deionized water until neutral, add 30 mg of dopamine hydrochloride, and sonicate for 30 minutes to obtain amino-functionalized magnetic nanoparticles B with an average particle size of 10 nm.

Embodiment 3

[0061] To 16 mg of aldehyde functionalized Fe 3 o 4 Add 9 ml of phosphate buffer and 30 mg of lipase, shake at 0-4°C for 6 hours, and obtain the immobilized enzyme number C after magnetic separation and washing. And calculate the amount of enzyme immobilization by measuring the protein content and enzyme activity in the solution before and after immobilization (see figure 2 ).

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Abstract

The invention relates to a functionalized magnetic nano particle immobilized enzyme and a preparation method thereof. Bisphenols with active functional groups are used to coordinate with defect sites on the surface of magnetic nanoparticles, thereby introducing active functional groups on the surface of magnetic nanoparticles, and forming covalent bonds with enzymes to achieve the purpose of immobilizing enzymes. This type of immobilized enzyme is easy to prepare, easy to operate, and exhibits high reactivity and chiral selectivity in chiral resolution and chiral synthesis. This type of immobilized enzyme is also very stable, and can still maintain high reactivity and chiral selectivity after repeated use. The method is universal and applicable to the immobilization of other biomacromolecules.

Description

technical field [0001] The invention relates to a functionalized magnetic nano particle immobilized enzyme. [0002] The present invention also relates to the preparation method of the above-mentioned functionalized magnetic nano particle immobilized enzyme. [0003] The present invention also relates to the application of the functionalized magnetic nanoparticles immobilized enzyme. Background technique [0004] As a biocatalyst, enzymes are widely used in food processing, medicine and fine chemical industries due to their high selectivity, mild catalytic conditions and no pollution. However, natural enzymes have poor stability, are easily inactivated, and cannot be reused, and the separation and purification of products are difficult, making it difficult for large-scale application in industrial catalysis. In recent years, immobilization to improve the stability and use times of enzymes has become a research hotspot. [0005] Magnetic nanomaterials have broad applicatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12P41/00
Inventor 李灿张艳梅李军
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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