Bitarget fibroblast growth factor acceptor and transgene carrier of integrated element

A growth factor receptor, fibroblast technology, applied in gene therapy, genetic engineering, plant genetic improvement and other directions, to achieve the effect of inhibiting tumor growth and good application prospects

Inactive Publication Date: 2006-04-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At present, the RGD peptide-coupled PEI that uses bFGF functional domain peptide molecules to simulate integrin ligands has not been reported yet.

Method used

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  • Bitarget fibroblast growth factor acceptor and transgene carrier of integrated element
  • Bitarget fibroblast growth factor acceptor and transgene carrier of integrated element
  • Bitarget fibroblast growth factor acceptor and transgene carrier of integrated element

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The polypeptide is coupled to PEI through a heterobifunctional crosslinker SPDP (Succinimidyl 3-(2-pyridyldithio) propionate). SPDP interacts with the amino groups of PEI (pH7-8) to form pyridinedithiol-modified PEI (PDP-PEI); the latter can react with proteins containing sulfhydryl groups. The polypeptide reacts with PDP-PEI via the cysteine ​​sulfhydryl group to produce a disulfide-linked oligopeptide-PEI derivative. Take CR16 and CP9 peptides with different molar ratios to PEI to form coupling products, characterize the complexes by various physical and chemical means, and then combine with negatively charged exogenous DNA according to a certain N / P ratio through electrostatic interaction to form CR16 peptide / CP9 peptide / PEI / exogenous DNA carrier complex, and then apply the transfection complex to in vitro and in vivo experiments to determine the transgenic effect and determine the transgenic conditions.

[0027] 1. Synthesis of CR16 and CP9 peptides:

[0028] The...

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PUM

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Abstract

A transgenic carrier with deal targets (fibroblast growth factor receptor and integrant) is composed of the polypeptide CR16 specifically linked with alkaline fibroblast growth factor receptor, the polypeptide CP9 specifically linked with integrant, and the transgenic non-virus carrier system of CR16/CP9/cationic polymer PEI/exogenous DNA. Said carrier can effectively introduce the exogenous DNA to the tumor cell line and tumor tissue with high expression of FGFRs, so suppressing the growth of tumor. It can be used to preparation of the medicine for treating tumor and other diseases.

Description

technical field [0001] The invention belongs to the field of biotechnology, and constructs a polyethylenimine (PEI) as the basic skeleton, combined targeting fibroblast growth factor receptors (fibroblast growth factor receptors, FGFRs) and integrins on the surface of tumor cells (integrin) is a non-viral transgene vector used to transfer expression plasmids encoding reporter genes and therapeutic genes into tumor cells that highly express FGFR and integrin receptors. The invention also relates to the preparation method and application of the non-viral transgene carrier. Background technique [0002] Gene therapy refers to the transfer of exogenous genes into cells, through the restoration or increase of gene expression to correct the structural or functional disorders of human genes, to prevent the progression of disease, to kill diseased cells, or to inhibit foreign pathogens The replication of genetic material, so as to achieve the purpose of treating diseases. Gene the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/63C12N15/12C07K14/435A61P35/00A61K48/00
Inventor 王青青李达李经忠汤谷平余海曹雪涛
Owner ZHEJIANG UNIV
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