Sound sensitive lipid nanoparticle as well as application and preparation method thereof

A lipid nanoparticle, sound-sensitive technology, applied in the field of biomedicine, can solve problems such as unclear related mechanisms, and achieve the effects of inhibiting the growth of HepG2 cells, high drug loading, and stable properties.

Active Publication Date: 2018-11-23
CHONGQING MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

A large number of studies have shown that the biological effects produced in sonodynamic therapy are closely related to their frequency and intensity, but the related mechanism is still unclear and needs to be further explored

Method used

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  • Sound sensitive lipid nanoparticle as well as application and preparation method thereof
  • Sound sensitive lipid nanoparticle as well as application and preparation method thereof
  • Sound sensitive lipid nanoparticle as well as application and preparation method thereof

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Embodiment Construction

[0034] The following is a further detailed description through specific implementations:

[0035] The embodiment is basically as attached Figure 1 to Figure 8 Shown:

[0036] 1. The method for preparing the sound-sensitive lipid nanoparticles of the present invention, the specific steps are as follows:

[0037] A. Precisely weigh out 1mg dilauroyl lecithin, 4mg dipalmitoylphosphatidylcholine, 2mg distearoyl-sn-glycerol-3-phosphoglycerol, 1.5mg distearoylphosphatidylethanolamine-polyethylene glycol 2000, 1.5mg cholesterol and 1mg hematoporphyrin monomethyl ether (HMME) in a round-bottomed flask, under dark conditions, add appropriate amount of chloroform and methanol to fully dissolve;

[0038] B. Place the round bottom flask on a rotary evaporator and evaporate under reduced pressure for 1 hour to form a uniform dark red film;

[0039] C. Accurately weigh 1 mg of doxorubicin hydrochloride (DOX) and dissolve it in 4 mg of phosphate buffered saline (PBS), and add it to the above round b...

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Abstract

The invention relates to the technical field of biological medicine, and discloses a sound sensitive lipid nanoparticle. The sound sensitive lipid nanoparticle is prepared from lipid carriers, HMME (hematoporphyrin monomethyl ether), perfluorocty bromide and adriamycin, wherein the mass percentage of the HMME is 2.86 to 3.69 weight percent; the mass percentage of the adriamycin is 1.28 to 2.05 percent. The HMME and coated PFOB and DOX carried sound sensitive lipid nanoparticle is successfully prepared; the LIFU control medicine release is realized; the sound power and chemotherapy are combinedfor enhancing the HepG2 cell growth and in-vitro CT imaging; the foundation is provided for the later-period in-vivo imaging and treatment.

Description

Technical field [0001] The invention relates to the technical field of biomedicine, in particular to a sound-sensitive lipid nanoparticle, an application and a preparation method thereof. Background technique [0002] As a widely used drug carrier, liposomes have good biocompatibility. The bilayers of liposomes can hold hydrophobic substances, and the inside can be loaded with hydrophilic substances. The lipid nanoparticles mainly use solid lipids or a small amount of liquid oil at room temperature as the carrier, and the drug is dispersed or encapsulated in the lipid core to form a solid colloidal particle drug delivery system with a particle size of about 10 to 1000 nm to form a lipid Nano drug delivery system. Lipid nanoparticles have good biocompatibility, can improve the stability of unstable drugs, and have slow and controlled release and long-acting effects. A certain proportion of liquid oil or mixed lipids can be used as components to overcome the shortcomings of solid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/04A61K9/127A61K47/24A61K41/00A61K31/704A61P35/00
CPCA61K9/1271A61K31/704A61K41/0028A61K41/0033A61K47/24A61K49/0433A61P35/00A61K2300/00
Inventor 郭丹
Owner CHONGQING MEDICAL UNIVERSITY
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