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A kind of sound-sensitive lipid nanoparticle, application and preparation method thereof

A lipid nanoparticle, sound-sensitive technology, applied in the field of biomedicine, can solve problems such as unclear related mechanisms, and achieve the effect of inhibiting the growth of HepG2 cells, regular morphology, and good CT imaging effect.

Active Publication Date: 2021-04-13
CHONGQING MEDICAL UNIVERSITY
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Problems solved by technology

A large number of studies have shown that the biological effects produced in sonodynamic therapy are closely related to their frequency and intensity, but the related mechanism is still unclear and needs to be further explored

Method used

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  • A kind of sound-sensitive lipid nanoparticle, application and preparation method thereof
  • A kind of sound-sensitive lipid nanoparticle, application and preparation method thereof
  • A kind of sound-sensitive lipid nanoparticle, application and preparation method thereof

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Embodiment Construction

[0034] The following is further described in detail through specific implementation methods:

[0035] The embodiment is basically as attached Figure 1 to Figure 8 Shown:

[0036] One, the preparation method of the sound-sensitive type lipid nanoparticle of the present invention, concrete steps are as follows:

[0037] A. Accurately weigh 1 mg dilauroyl lecithin, 4 mg dipalmitoyl phosphatidylcholine, 2 mg distearoyl-sn-glycerol-3-phosphoglycerol, 1.5 mg distearoyl phosphatidylethanolamine-polyethylene glycol In 2000, put 1.5mg of cholesterol and 1mg of hematoporphyrin monomethyl ether (HMME) in a round bottom flask, under dark conditions, add appropriate amount of chloroform and methanol to fully dissolve;

[0038] B. Place the round bottom flask on a rotary evaporator to evaporate under reduced pressure for 1 hour to form a uniform dark red film;

[0039] C. Accurately weigh 1mg of doxorubicin hydrochloride (DOX) and dissolve it in 4mg of phosphate buffered saline (PBS), a...

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Abstract

The invention relates to the technical field of biomedicine, and discloses a sound-sensitive lipid nanoparticle, which is composed of a lipid carrier, hematoporphyrin monomethyl ether, perfluorooctyl bromide and doxorubicin, wherein hematoporphyrin monomethyl ether and doxorubicin are respectively 2.86-3.69wt% and 1.28-2.05wt%. The invention successfully prepared acoustic-sensitive lipid nanoparticles loaded with HMME and wrapped with PFOB and DOX, realized LIFU controlled drug release, combined with sonodynamics and chemotherapy to enhance the inhibition of HepG2 cell growth and in vitro CT imaging, and provided a basis for later in vivo imaging and treatment. Base.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a sound-sensitive lipid nanoparticle, its application and its preparation method. Background technique [0002] As a widely used drug carrier, liposome has good biocompatibility. It can accommodate hydrophobic substances between its bilayers and can be loaded with hydrophilic substances inside. Lipid nanoparticles mainly use solid lipid or a small amount of liquid oil at room temperature as the carrier, and the drug is dispersed or wrapped in the lipid core to form a solid micelle drug delivery system with a particle size of about 10-1000 nm, forming a lipid drug delivery system. nano drug delivery system. Lipid nanoparticles have good biocompatibility, can improve the stability of unstable drugs, and have slow and controlled release and long-acting effects. A certain proportion of liquid oil or mixed lipid can be used as a component to overcome the shortcomings of solid li...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/04A61K9/127A61K47/24A61K41/00A61K31/704A61P35/00
CPCA61K9/1271A61K31/704A61K41/0028A61K41/0033A61K47/24A61K49/0433A61P35/00A61K2300/00
Inventor 冉海涛郭丹汪星月
Owner CHONGQING MEDICAL UNIVERSITY
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