Method for preparing small-sized nano-hydroxyapatite by using carbon dots (CDs) as template

A nano-hydroxyapatite, small-size technology, applied in the direction of nanotechnology, chemical instruments and methods, phosphorus compounds, etc., can solve the problems that are not suitable for the influence of cancer cells, and achieve the effect of reducing the size

Inactive Publication Date: 2018-09-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the hydroxyapatite particles prepared by the template method are generally spherical, and the size is mostly above 200nm, which is not suitable for analyzing the effect of the size effect of nanoparticles on cancer cells.

Method used

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  • Method for preparing small-sized nano-hydroxyapatite by using carbon dots (CDs) as template
  • Method for preparing small-sized nano-hydroxyapatite by using carbon dots (CDs) as template
  • Method for preparing small-sized nano-hydroxyapatite by using carbon dots (CDs) as template

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Preparation of carbon dots (CDs):

[0034] Weigh H at a molar ratio of 1:1 2 N(CH 2 ) 10 COOH and NaOH solids were neutralized by adding 25 mL of deionized water, and centrifuged to remove impurities. Then add 25 mL of H 2 N(CH 2 ) 10 A citric acid solution with an equimolar concentration of COOH was stirred vigorously for 30 minutes, and the white precipitate was collected and dried; finally, the dried sample was ground, placed in a muffle furnace at 300°C, thermally oxidized for 2 hours, and cooled naturally; the product after thermal oxidation was extracted by ultrasonic method It was dissolved in deionized water, centrifuged to remove insoluble precipitates, and then the product solution was dialyzed with a dialysis bag with a molecular weight cut-off of 3500D for 48 hours, and then freeze-dried to obtain a purified carbon dot product.

[0035] (2) Preparation of small-sized nano-hydroxyapatite:

[0036] First, measure 10 mL of Ca(NO 3 ) 2 solution, dil...

Embodiment 2

[0038] (1) The preparation of carbon dots (CDs) is the same as in Example 1.

[0039] (2) Preparation of small-sized nano-hydroxyapatite:

[0040] First, measure 10 mL of Ca(NO 3 ) 2 solution, dilute to 23.5mL with deionized water, and then adjust Ca(NO 3 ) 2 The pH of the solution was brought to 10.5. Then slowly add 1.5mL of CDs with a concentration of 24.4g / L to it, keeping the volume of the calcium source solution at 25mL with a concentration of 0.2mol / L; when the CDs-Ca complex precipitate is evenly dispersed, slowly add 30mL of CDs with a concentration of 0.1mol / LNa 3 PO 4 solution; then the mixed solution was transferred into a polytetrafluoroethylene autoclave, reacted at 180°C under hydrothermal conditions for 8h, cooled naturally to room temperature, washed with deionized water and ethanol for 3 times, dried in an oven at 50°C, and ground to obtain Small size nano-hydroxyapatite S3.

Embodiment 3

[0042] (1) The preparation of carbon dots (CDs) is the same as in Example 1.

[0043] (2) Preparation of small-sized nano-hydroxyapatite:

[0044] First, measure 10 mL of Ca(NO 3 ) 2solution, dilute it to 22.5mL with deionized water, and then adjust the Ca(NO 3 ) 2 The pH of the solution was brought to 10.5. Then slowly add 2.5mL of CDs with a concentration of 24.4g / L to it, keeping the volume of the calcium source solution at 25mL with a concentration of 0.2mol / L; until the CDs-Ca complex precipitate is evenly dispersed, slowly add 30mL of CDs with a concentration of 0.1mol / LNa 3 PO 4 solution; then the mixed solution was transferred into a polytetrafluoroethylene autoclave, reacted at 180°C under hydrothermal conditions for 8h, cooled naturally to room temperature, washed with deionized water and ethanol for 3 times, dried in an oven at 50°C, and ground to obtain Small size nano-hydroxyapatite S4.

[0045] The transmission electron microscope figure (TEM) of the nan...

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Abstract

The invention belongs to the field of nano-biological materials, and discloses a method for preparing small-sized nano-hydroxyapatite by using carbon dots (CDs) as a template. The method comprises thefollowing steps: weighing solids H2N(CH2)10COOH and NaOH and dissolving in deionized water, then adding a citric acid solution and stirring for reacting, collecting white precipitate, drying, grinding, and then carrying out a thermal oxidization reaction at 300 DEG C to obtain the CDs; adjusting the pH of a Ca(NO3)2 solution to 10.5 with an alkaline solution, then dropwise adding the CDs to carryout a complexation precipitation reaction, dropwise adding an Na3PO4 solution, carrying out a hydrothermal reaction at 140 to 180 DEG C, and washing and drying a product to obtain the small-sized nano-hydroxyapatite. Hydroxyapatite nanoparticles prepared by the method are shaped like short rods, are smaller in size than particles without the CDs, can be reduced in particle size through the increase of the CD content, and have a potential application prospect in the field of nanomedicine.

Description

technical field [0001] The invention belongs to the field of nano-biological materials, and in particular relates to a method for preparing small-sized nano-hydroxyapatite by using carbon dots as templates. Background technique [0002] Hydroxyapatite is a kind of bioactive material, which has a mineral composition similar to that of human bone and has good biocompatibility. In addition, hydroxyapatite also has good osteoconductivity, which makes it widely used in the field of biomedicine for the repair and replacement of human hard tissue injuries and defects. [0003] In recent years, hydroxyapatite particles have also been widely used in the delivery of drugs, proteins, nucleic acids, etc. However, some studies have found that nano-scale hydroxyapatite has the advantages of small size and large specific surface area, and it can be used as a nano-drug to inhibit cancer cell proliferation and promote cancer cell apoptosis. Existing literature and data have reported that d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B25/32B82Y40/00
CPCB82Y40/00C01B25/325C01P2002/72C01P2002/82C01P2004/04C01P2004/16
Inventor 邓春林杨慧方
Owner SOUTH CHINA UNIV OF TECH
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