64 results about "Superovulations" patented technology

Improved insemination systems particularly adapted to use for sex-selected sperm sorting include systems which achieve superovulation and then multiple embryo production with sexed embryos. These systems combine with other techniques, including techniques for enhanced sheath fluid and other strategies which minimize stress on the sperm cells, and, potentially, a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial application of sperms samples as well as the resulting animals may be achieved.

The invention relates to a method of producing a protein of interest, comprising making a non-human transgenic mammal that produces said protein in its milk, obtaining said milk from the non-human transgenic mammal and purifying said protein of interest from the milk. Transgenic bovine animals were generated, which are able to produce human growth hormone in mammary glands. The method involves cloning of a genetic construct encoding hGH gene and beta casein promoter conveniently in an expression vector. It also includes transfection procedures into fetal bovine somatic cells, generally fibroblasts, and the nuclear transfer into enucleated bovine oocytes, generating thus transgenic embryos. The method also includes other procedures to generate transgenic embryos for the further expansion of the transgenic herd, such as the subcloning of transgenic female bovines, the superovulation of transgenic cows and their insemination with semen from a non-transgenic or a transgenic male bovine, and the superovulation of non-transgenic cows and their insemination with semen from a transgenic male bovine. Afterwards, transgenic embryos give rise to transgenic cattle that produce human growth hormone in huge amounts in their milk, from which the hormone is completely purified and analysed to fulfill all the requirements for the manufacture of a pure biopharmaceutical product.

The invention discloses an in vitro embryo production method by lamb superovulation oocytes. The production method provided by the invention is accomplished by the following technical scheme: carrying out in vitro culture on oocytes acquired from lamb body in a maturation medium and maturing into ova, carrying out in vitro fertilization and culture so as to make the ova grow into embryos, and transplanting survived embryos into a surrogate ewe body for breeding. In comparison with the prior art, the method provided by the invention is utilized to adopt the oocytes acquired after the lamb superovulation treatment for the in vitro embryo production and has advantages of large quantity of the acquired oocytes, high production efficiency of embryo and short time for breeding lamb offspring. Therefore, the method provided by the invention has an edge in the application of large scale in vitro embryo production.

The invention relates to application of an LHCGR gene of a Chinese Holstein cow used as a molecular marker. The LHCGR gene of the Chinese Holstein cow is positioned at a Reference SNP Cluster Report: rs41256848 site; and when the site is homozygosized into 'G', the advantage of superovulation is shown. The LHCGR gene serving as the molecular marker is used for the assistant breeding of the Chinese Holstein cow, so that the generation interval can be shortened and the breeding process can be improved.

The present invention provides methods of producing biologically active recombinant bFSH and methods of increasing reproduction in mammals, particularly bovine, using recombinant bFSH. Also provided are methods of producing single chain recombinant bFSH. The recombinant bFSH of the present invention increases superovulation, embryo development, and reproductive efficiency in cattle and other ungulates.

The invention discloses a superovulation method for goats. The superovulation method comprises the following steps of: selecting female goats which can be applied to superovulation; teasing in the morning and at the night every day; counting from day zero when oestrum is observed for the first time; injecting follicle stimulating hormone once in the morning and at the night on the fourth day, the fifth day, the sixth day and the seventh day respectively; injecting cloprostenol during the injection of the follicle stimulating hormone in the morning and at the night on the seventh day; injecting luteotropin on the eighth day; performing semen deposition through a laparoscope; and recycling a pronuclear embryo on the tenth day or collecting a mulberry body on the sixteenth day. A progesterone needing process in the conventional superovulation method for goats is eliminated, production time is shortened, and production cost is reduced; simultaneously, with the method, superovulation efficiency which is the same as that of the conventional superovulation method can be achieved and an experimental result of each batch indicates that each goat can obtain 8 to 30 embryos on average.

The invention relates to a method for increasing sow ovulation rate and injection thereof, belonging to the technical field of agricultural biological products. The method of simultaneously injecting PG (cloprostenol sodium) and PMSG (antostab) or a mixture of PG and PMSG before oestrum, and injecting HCG (chorionic corticotrophin) or LHRH-A3 (luteinizing hormone releasing hormone A3) during oestrum is adopted to promote the ovulation rate of young sows or multiparity sows to be 2-3 times of normal ovulation amount, thus improving reproductive performance of sows and obviously increasing the number of young pigs borne by the sows. The method has stable technology and obvious effect, and can not induce ovarian cyst; by using the method, the same superovulation effect can be achieved on standby sows ay any time before sexual maturity.

The present invention provides methods of producing biologically active recombinant eFSH analogs and methods of increasing reproduction in mammals, particularly equine, using recombinant eFSH analogs. Also provided are methods of producing biologically active single chain recombinant eFSH. The recombinant eFSH analogs of the present invention increase superovulation, follicular development, embryo development, and reproductive efficiency in horses, cattle and other ungulates.

The present invention provides a novel cow superovulation sustained-release agent, which is a hyaluronic acid solution with a concentration of 5.0-10.0 mg / mL. According to the present invention, with the combination of the cow superovulation sustained-release agent and FSH, the FSH action effect is prolonged, the cow superovulation process is simplified, the superovulation efficiency is improved, and the superovulation effect equal to the conventional superovulation effect can be obtained, wherein 8 injections within 4 days is reduced to 2 injections within 4 days; and the operation of the method is simple, the superovulation efficiency is improved, and the method is easy to operate at the cow farming site and is easy to promote and use.

The invention discloses a continuous superovulation of cow, which comprises the following steps: placing cork in the zero day, injecting once follitropin in the forth, fifth, sixth and seventh days, injecting once cloprostenol in the morning and evening of seven day, fertilizing artificially in the ninth and tenth days, brushing embryo in the seventh day, injecting cloprostenol, injecting cloprostenol in the twentieth-forth day, placing cork in the thirty day, detecting through Ultrasonic B.

The invention relates to a method for superovulation of young dairy cattle with high intensity repeatedly, belonging to the technical field of livestock reproduction. The method comprises adjustment of dietary nutrition standards before superovulation treatment of donor cattle, pretreatment of reproductive system, superovulation hormone treatment program and multiplicative process after repeated superovulation. The superovulation time of donor cattle is advanced to be 11.5 age of the moon, and the superovulation interval is shortened to be 21-26 days; the reproductive potential of various cows before reproductive life can be fully developed, thereby obtaining higher economic benefit without influencing subsequent natural reproductive capacity.

The invention discloses a method for preparing an inhibin genetic engineering regulation antigen, which comprises the following steps: cloning an inhibin encoding gene; making use of the inhibin encoding gene to prepare an inhibin recombinant fusion protein; and making use of the inhibin recombinant fusion protein to prepare the inhibin genetic engineering regulation antigen. The method for preparing the inhibin genetic engineering regulation antigen has the advantages of simplicity, high purity, good immunogenicity, low price and the like; the inhibin genetic engineering regulation antigen prepared by the method does not have toxicity, pathogenicity and drug residue, is harmless to human and animals, has no adverse effect on consumers, can improve the ovulation rate and the litter size, improve utilization ratio of a superovulation embryo, promote the sexual maturation so as to improve the reproduction and the like.

The present invention provides a reproductive performance improving feed for sows and a preparation method thereof. The reproductive performance improving feed comprises the following raw materials: soybean meal, peanut meal, wheat bran, stone powder, compound vitamins, mixed trace elements, calcium hydrophosphate, compound enzyme preparations, micro-ecological preparations, beta-1,3-D-glucan, a Chinese herbal medicinal extract, lysine, threonine, choline chloride, an antioxidant and edible salt. The provided feed for the sows can improve the antibody level of the sows, enhances the disease resistance of the body, effectively promotes the superovulation of the sows, reduces the estrus returning rate after breeding, increases the litter sizes and the weight of weaning pigs, the survival rate and the daily weight gain.

The invention relates to a method of producing a protein of interest, comprising making a non-human transgenic mammal that produces said protein in its milk, obtaining said milk from the non-human transgenic mammal and purifying said protein of interest from the milk. Transgenic bovine animals were generated, which are able to produce human growth hormone in mammary glands. The method involves cloning of a genetic construct encoding hGH gene and beta casein promoter conveniently in an expression vector. It also includes transfection procedures into fetal bovine somatic cells, generally fibroblasts, and the nuclear transfer into enucleated bovine oocytes, generating thus transgenic embryos. The method also includes other procedures to generate transgenic embryos for the further expansion of the transgenic herd, such as the subcloning of transgenic female bovines, the superovulation of transgenic cows and their insemination with semen from a non-transgenic or a transgenic male bovine, and the superovulation of non-transgenic cows and their insemination with semen from a transgenic male bovine. Afterwards, transgenic embryos give rise to transgenic cattle that produce human growth hormone in huge amounts in their milk, from which the hormone is completely purified and analysed to fulfill all the requirements for the manufacture of a pure biopharmaceutical product.

The invention relates to a method of producing a protein of interest, comprising making a non-human transgenic mammal that produces said protein in its milk, obtaining said milk from the non-human transgenic mammal and purifying said protein of interest from the milk. Transgenic bovine animals were generated, which are able to produce human growth hormone in mammary glands. The method involves cloning of a genetic construct encoding hGH gene and beta casein promoter conveniently in an expression vector. It also includes transfection procedures into fetal bovine somatic cells, generally fibroblasts, and the nuclear transfer into enucleated bovine oocytes, generating thus transgenic embryos. The method also includes other procedures to generate transgenic embryos for the further expansion of the transgenic herd, such as the subcloning of transgenic female bovines, the superovulation of transgenic cows and their insemination with semen from a non-transgenic or a transgenic male bovine, and the superovulation of non-transgenic cows and their insemination with semen from a transgenic male bovine. Afterwards, transgenic embryos give rise to transgenic cattle that produce human growth hormone in huge amounts in their milk, from which the hormone is completely purified and analysed to fulfill all the requirements for the manufacture of a pure biopharmaceutical product.

The present invention relates to methods, compositions and kits for controlling, managing or manipulating the non-human mammalian female reproductive cycle. The methods include oestrus induction and / or synchronisation, induction of ovulation and / or superovulation and optionally further includes a method of providing an immunopermissive uterine environment prior to insemination or implantation of embryos.

The invention establishes an efficient method for producing an in-vitro calf embryo, and the method comprises the technology of efficient oocyte in-vitro maturation, in-vitro fertilization, in-vitro embryo cultivation and the like. In the production of the in-vitro calf embryo, the oocyte in a calf ovary can be completely taken as abundant embryo sources, and superovulation and collection embryo are carried out on the calf. The method is combined with the cow female-controlled technology and uses a female-controlled X or Y sperm selectively to perform in-vitro fertilization according to a breeding object, so as to produce a fine cow embryo with definite blood relationship; and after transplantation, the produced calf can be directly supplemented to a high-yield cow core group or be used for breeding a reserved bull.

The invention relates to a method of producing a protein of interest, comprising making a non-human transgenic mammal that produces said protein in its milk, obtaining said milk from the non-human transgenic mammal and purifying said protein of interest from the milk. Transgenic bovine animals were generated, which are able to produce human growth hormone in mammary glands. The method involves cloning of a genetic construct encoding hGH gene and beta casein promoter conveniently in an expression vector. It also includes transfection procedures into fetal bovine somatic cells, generally fibroblasts, and the nuclear transfer into enucleated bovine oocytes, generating thus transgenic embryos. The method also includes other procedures to generate transgenic embryos for the further expansion of the transgenic herd, such as the subcloning of transgenic female bovines, the superovulation of transgenic cows and their insemination with semen from a non-transgenic or a transgenic male bovine, and the superovulation of non-transgenic cows and their insemination with semen from a transgenic male bovine. Afterwards, transgenic embryos give rise to transgenic cattle that produce human growth hormone in huge amounts in their milk, from which the hormone is completely purified and analysed to fulfill all the requirements for the manufacture of a pure biopharmaceutical product.

The invention discloses a breeding raising method for meat sheep. The method is characterized by comprising the steps: by taking poll dorset sheep as a male parent and small tailed han sheep as a female parent, carrying out hybridization, wherein the propagative technologies mainly comprise estrus control, hormone treatment, biological stimulation, vaginal sponge method and prostatic hormone method to promote superovulation, a tit double-lamb agent or a twinborn-medicament, which is an aqueous agent and an oil agent, is adopted; 1 ml of the aqueous agent is subcutaneously injected into the neck of each of the male sheep once in 5 weeks and 2 weeks before mating; and 2 ml of the oil agent product is intramuscularly injected at the buttock in 2 weeks before mating. Twins and polyembryony are induced. Fundamental hybridization improvement is carried out from biological characteristics, artificial raising environment selection, raising variety selection, propagative technical improvement, raising feed research, fattening management technologies and disease diagnosis, prevention and treatment, and the meat sheep variety is bred, so that implementation of industrialization of the meet sheep is promoted.

The invention relates to a modified method for picking ova in live cattle. Particularly in one aspect, the invention relates to an FSH (follicle-stimulating hormone) preparation, which is an aqueous solution containing a bovine follicle-stimulating hormone having a concentration of 25-50 mu g / ml; in the other aspect, the invention relates to application of the FSH in the preparation of preparations for picking ova in live cattle. The steps of picking ova in live cattle include: carrying out superovulation before picking, picking ova in live cattle, and detecting oocytes. The modified method of the invention has the excellent advantages described as in the description, such as availability for acquiring a high ratio of available oocytes.

Improved insemination systems particularly adapted to use for sex-selected sperm sorting include systems which achieve superovulation and then multiple embryo production with sexed embryos. These systems combine with other techniques, including techniques for enhanced sheath fluid and other strategies which minimize stress on the sperm cells, and, potentially, a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial application of sperms samples as well as the resulting animals may be achieved.

The invention provides an application of letrozole (LE) in preparation of a drug used for causing an adult cattle to excessively ovulate. According to the invention, LE is added in the traditional adult cattle superovulation scheme, and follicular development is promoted by utilizing influence of letrozole on internal secretion and paracrine and an ovary follicle collecting effect, so that quantity of available ovocyte is increased; besides, a half-life period of letrozole is shorter, metabolism velocity is high, no side effect is produced, and letrozole can serve as a livestock superovulation auxiliary drug to be popularized and applied in animal husbandry production.

The invention discloses a cultivation method of beef cattle hybridized by Charolais cattle, Simmental cattle and local cattle. The method includes: selecting a Tongjiang cow as the female parent; performing superovulation on the Tongjiang cow; selecting Simmental breeding bull sperm to perform artificial insemination; collecting the embryo of the Tongjiang cow; selecting a Charolais surrogacy cowto perform embryo transplantation; detecting 2 genotypes related to the growth traits of a first-filial-generation cow; selecting an F1-generation cow as the female parent; performing superovulation on the F1-generation cow; selecting Simmental breeding bull sperm to perform artificial insemination; collecting the embryo of the F1-generation cow; selecting the first-filial-generation cow to perform embryo transplantation; detecting 2 genotypes related to the growth traits of a second-filial-generation individual; selecting the cattle, which is good in body shape and growth speed and has dominant genotypes, hybridized by Charolais cattle, Simmental cattle and local cattle, and using the artificial insemination, superovulation and embryo transplantation technologies to expand propagation. Bythe method, the workload of scientific and technical personnel can be lowered, and cultivation time can be shortened.

The present invention provides methods of producing biologically active recombinant bFSH and methods of increasing reproduction in mammals, particularly bovine, using recombinant bFSH. Also provided are methods of producing single chain recombinant bFSH. The recombinant bFSH of the present invention increases superovulation, embryo development, and reproductive efficiency in cattle and other ungulates.

The invention provides a simulated egg taking model and a using method and application thereof. On the model, a standard gynecological examination lithotomy position is from a waist and abdomen part to a 1 / 3 thigh position, the model is internally provided with female ovarian tissue, a vagina, a uterus and oviducts, and ovarian follicles are uniformly distributed on the surfaces of ovaries, and internally comprise simulated eggs. The simulated egg taking model simulates features of post-superovulation female ovarian tissue, trains egg-taking operation practice of clinical staff, increases thedegree of egg-taking skillfulness and the recovery rate, pushes medical development and reduces the pain and economic loss of a patient.

The present invention relates to a method for super-conventional breeding of fine-bred dairy cows using embryonic biotechnology, selecting excellent and suitable cows as donors, the method comprising the following steps: a, superovulation, b, artificial insemination c, embryo punching, d , Embryo inspection, e, embryo freezing, f, embryo thawing, g, embryo separation, h, embryo transfer. By adopting the above method, the birth rate of female calves can be effectively controlled, the production cost is reduced, the quality of dairy cows and milk is improved, and the purpose of supernormal rapid breeding of female dairy cows is achieved.

The invention discloses an induction and culture line establishment method for mammal parthenogenetic epiblast stem cells and an obtained parthenogenetic epiblast stem cell line. The method comprises the following steps: executing the superovulation to two varieties of female mice ICR and 129, and executing the parthenogenetic activation to ovocyte; after 5 h of the activation, cultivating for 96 h in KSOM, and generating blastaea, and executing the line establishment by using the blastaea. The line establishment method is capable of efficiently inducing mice parthenogenetic embryo to form the parthenogenetic epiblast stem cells pa-EpiLSCs, keeping the parthenogenetic diploid features and steady growing for more than 30 generations. The method is capable of providing a new tool for rapid generation of a recessive trait genetic model, and providing the new technical and theoretical basis for other mammals establishing the pa-EpiLSCs from the parthenogenetic embryos.

Described herein are methods for synchronizing ovulation in a herd, inducing superovulation in a single animal, and improving frequency of successful implantation and development of fertilized ova.

The invention discloses a making method of nanometer slow-release agent to simplify animal FSH superovulation in the embryo engineering technical domain, which comprises the following steps: 1) dissolving 0. 5%-3% fatty acid in the alkaline condition for 8-12h to make vesicle; 2) adjusting the grain size of vesicle and surface load; adding cationic surfactant in the vesicle liquid with weight rate of vesicle liquid and cationic surfactant at 1: 0. 05-0. 1; 3) adopting phosphoric acid buffer to adjust the grain size and surface load into 0. 05%-1. 5% vesicle slow-release agent. The invention simplifies the operation of superovulation without special request for instrument, which has strong repeatability.

The invention provides a high-efficiency breeding method of goats for achieving one birth of multiple goats. The method comprises the steps that (1), synchronous estrus regulation is conducted, and amethod of CDIR+PMSG is adopted; (2), superovulation regulation is conducted, and a method in which an FSH diminishing method cooperates with PG and lutropin to release hormone A3 is adopted; (3), studram hybridization is conducted; (4), nutrient regulation is conducted, and each goat is fed with dried grass and fine feed. According to the method, processing is conducted by using exterior hormonesso that the breeding of the goats can be improved to 3-4 goats for one birth, one birth of multiple goats is achieved to increase the goat production amount, the reproduction rate of the goat is greatly increased, economic benefits of goat raising is improved and the confident of farmers is improved. The method has the advantages that the cost is low, the operation is intramuscular injection which is simple and convenient, and subsequent complicated work is reduced by means of self hybridization of stud ram, which is beneficial for being popularized and used among the farmers.