Cattle continuous processes method
A technology of superovulation and superovulation, which is applied in the field of embryo engineering, can solve the problems of reduced use times, increased embryo transfer costs, and long time intervals, and achieves the effects of reducing production costs, reducing costs, and shortening intervals
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Embodiment 1
[0028] Embodiment 1: This embodiment is a preferred embodiment.
[0029] 1 reagent
[0030] Brogeramine; Lidocaine hydrochloride; Duchenne's phosphate buffer solution (PBS); 0.9% normal saline; Alcohol cotton ball; Penicillin (1.6 million units); Streptomycin (1 million units); PG 0.2mg / 2ml / support, purchased from Shanghai Institute of Family Planning; FSH10mg / bottle, purchased from Institute of Zoology, Chinese Academy of Sciences.
[0031] 2 experimental equipment
[0032] Required equipment: flushing tube (16#), straight scissors 18cm, curved hemostat 18cm, hair shears (18cm), through-core, beaker 500ml, filter, infusion stand, 10ml syringe, three-way tube, latex tube, clip, 1ml syringe, wash bottle 500ml.
[0033] Required instruments: liquid nitrogen tank, embryo transfer vehicle, B-ultrasound instrument.
[0034] 4 operation steps
[0035] 4.1 Selection of donor cows. Dairy cows should be over 18 months old, beef cattle should reach 65%-70% of their mature body we...
Embodiment 2,3
[0064] The method is as in Example 1, and the dosage for injection is shown in Table 5. Embodiment 2, 3 also can reach the same effect as embodiment 1.
Embodiment 2
[0065] Example 2
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