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Cattle continuous processes method

A technology of superovulation and superovulation, which is applied in the field of embryo engineering, can solve the problems of reduced use times, increased embryo transfer costs, and long time intervals, and achieves the effects of reducing production costs, reducing costs, and shortening intervals

Inactive Publication Date: 2007-02-28
BEIJING JINXIU DADI AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The long time interval has resulted in a reduction in the use of excellent donor cattle, which has virtually increased the cost of embryo transfer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: This embodiment is a preferred embodiment.

[0029] 1 reagent

[0030] Brogeramine; Lidocaine hydrochloride; Duchenne's phosphate buffer solution (PBS); 0.9% normal saline; Alcohol cotton ball; Penicillin (1.6 million units); Streptomycin (1 million units); PG 0.2mg / 2ml / support, purchased from Shanghai Institute of Family Planning; FSH10mg / bottle, purchased from Institute of Zoology, Chinese Academy of Sciences.

[0031] 2 experimental equipment

[0032] Required equipment: flushing tube (16#), straight scissors 18cm, curved hemostat 18cm, hair shears (18cm), through-core, beaker 500ml, filter, infusion stand, 10ml syringe, three-way tube, latex tube, clip, 1ml syringe, wash bottle 500ml.

[0033] Required instruments: liquid nitrogen tank, embryo transfer vehicle, B-ultrasound instrument.

[0034] 4 operation steps

[0035] 4.1 Selection of donor cows. Dairy cows should be over 18 months old, beef cattle should reach 65%-70% of their mature body we...

Embodiment 2,3

[0064] The method is as in Example 1, and the dosage for injection is shown in Table 5. Embodiment 2, 3 also can reach the same effect as embodiment 1.

Embodiment 2

[0065] Example 2

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PUM

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Abstract

The invention discloses a continuous superovulation of cow, which comprises the following steps: placing cork in the zero day, injecting once follitropin in the forth, fifth, sixth and seventh days, injecting once cloprostenol in the morning and evening of seven day, fertilizing artificially in the ninth and tenth days, brushing embryo in the seventh day, injecting cloprostenol, injecting cloprostenol in the twentieth-forth day, placing cork in the thirty day, detecting through Ultrasonic B.

Description

technical field [0001] The invention relates to the field of embryo engineering, in particular to a continuous superovulation method for cattle. Background technique [0002] Cows are single-born animals, and only one egg is ovulated in one estrus cycle. During pregnancy, there is no estrus and ovulation. In actual production, due to the influence of factors such as pregnancy, lack of lactation, winter rest and the limitation of the actual utilization of cows, most cows Cows only ovulate dozens of eggs in their lifetime, which is less than 0.1% of the number of ovarian oocytes, and 99.9% of the oocytes have not matured and have not played their due role. Superovulation technology uses reproductive hormones to stimulate the growth and development of follicles, so that cows can produce oocytes several times to ten times more than normal, and cow embryos can be formed after artificial insemination. [0003] At present, in countries with developed animal husbandry, the technolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D19/00A01K29/00
Inventor 李海洋张红霞吴亦芳张向利王月李荣旗
Owner BEIJING JINXIU DADI AGRI CO LTD
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