Superovulation method for goats

A superovulation and goat technology, applied in the field of superovulation, can solve the problems of high price, increase cost, randomness limitation, etc., and achieve the effect of shortening production time and reducing production cost.

Inactive Publication Date: 2011-05-25
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

The use of these methods requires progesterone suppository to suppress estrus or induce estrus in the early stage. The treatment time is 5-14 days, which limits the randomness of the experiment. The price of suppositories is

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  • Superovulation method for goats

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specific Embodiment approach

[0015] The specific implementation steps are as follows:

[0016] Materials used:

[0017] Follicle-stimulating hormone (FSH), purchased from Ningbo Sansheng Pharmaceutical Co., Ltd., the specification is 100IU / support;

[0018] Cloprostenol (PG), purchased from Shanghai Family Planning Research Institute, the specification is 0.2mg / 2ml;

[0019] Luteinizing hormone (LH), purchased from Ningbo Sansheng Pharmaceutical Co., Ltd., the specification is 200IU / support.

[0020] 1. Identification of estrous sheep

[0021] Sheep for superovulation were selected, and estrus was conducted three times in the morning, middle and evening during the experiment period, that is, rams after vasectomy were selected and placed in the ewe pen.

[0022] 2. Superovulation of estrous sheep

[0023] When each ewe is in estrus, it is the 0th day (early, middle and late observation of estrus is acceptable), and on the fourth day, fifth day, sixth day, and seventh day, respectively, inject follicle-...

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Abstract

The invention discloses a superovulation method for goats. The superovulation method comprises the following steps of: selecting female goats which can be applied to superovulation; teasing in the morning and at the night every day; counting from day zero when oestrum is observed for the first time; injecting follicle stimulating hormone once in the morning and at the night on the fourth day, the fifth day, the sixth day and the seventh day respectively; injecting cloprostenol during the injection of the follicle stimulating hormone in the morning and at the night on the seventh day; injecting luteotropin on the eighth day; performing semen deposition through a laparoscope; and recycling a pronuclear embryo on the tenth day or collecting a mulberry body on the sixteenth day. A progesterone needing process in the conventional superovulation method for goats is eliminated, production time is shortened, and production cost is reduced; simultaneously, with the method, superovulation efficiency which is the same as that of the conventional superovulation method can be achieved and an experimental result of each batch indicates that each goat can obtain 8 to 30 embryos on average.

Description

[technical field] [0001] The invention relates to the technical field of superovulation, in particular to a method for superovulation of goats. [Background technique] [0002] Superovulation technology is a method of using exogenous gonadotropin to induce the development of multiple ovarian follicles and discharge eggs with fertilization ability, referred to as "superovulation". This technology, combined with embryo transfer technology, can greatly improve the production and multiplication capabilities of excellent breeding stock, and has been widely used in production and scientific research. [0003] Traditional goat superovulation began in the 1980s (Armstrong DT, Pfitzner AP, Warnes GM, Seamark RF. Superovulation treatments and embryo transfer in Angora goats. J Reprod Fertil 1983; 67: 403-10.; Tervit HR, Goold PG , McKenzie RD, Clarkson DJ, DrummondJ.Embryo transfers in Angora and Saanen goats.N Z Vet J 1985; 33:78-80), this method has been continued until now, the spe...

Claims

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Application Information

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IPC IPC(8): A61D19/00
Inventor 李运生张运海韩春杨章美玲随刘才曹鸿国章孝荣刘亚陶勇张远亮焦明慧季索菲贾晴张宇桂涛周娜汝刘晓蕊杨盼张子军丁建平
Owner ANHUI AGRICULTURAL UNIVERSITY
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