10 results about "Embryo freezing" patented technology

This invention provides a method for the cryopreservation and reconstitution of porcine embryos. During the in vitro maturation of porcine oocytes, porcine IVF embryo culture, and embryo freezing and thawing processes, the addition of TSA, 5-Aza-dC, Etomoxir, GSK-J4, and NR, either alone or in combination, improves the development and freezing efficiency of porcine IVF embryos. Experiments show that the oocyte maturation rate, embryo cleavage rate, and blastocyst rate of the groups adding TSA, 5-Aza-dC, and Etomoxir to the porcine oocyte in vitro maturation culture medium and porcine IVF embryo culture medium are significantly higher than those of the control group. Furthermore, the hatching rate of porcine IVF embryos after freezing and thawing is significantly higher in the groups adding GSK-J4 and NR to the freezing and thawing solutions than in the control group. This invention lays the foundation for the production of porcine in vitro fertilized embryos and related scientific research, providing an effective, safe, and feasible strategy for improving the production efficiency and quality of porcine in vitro embryos.

The liquid nitrogen box comprises a liquid nitrogen box body, the liquid nitrogen box body comprises an inner freezing heat preservation cavity and an outer protection shell, a first hole frame used for containing a freezing pipe is fixedly arranged at the bottom of the inner side of the inner freezing heat preservation cavity, and a second supporting assembly used for supporting the upper portion of the freezing pipe is arranged on the upper portion of the first hole frame. The second supporting assembly is connected with an adjusting assembly on the side wall of the inner freezing heat preservation cavity so as to adjust the vertical height of the second supporting assembly relative to the first hole frame, and a double-layer heat preservation cover is arranged in the liquid nitrogen box body and comprises an outer cover arranged on the upper portion of the liquid nitrogen box body and an inner heat preservation cover in threaded connection with the inner freezing heat preservation cavity. According to the utility model, the second supporting assembly with adjustable height is arranged, so that the liquid nitrogen box can accommodate embryo freezing tubes with different lengths, and a sample is ensured to be completely immersed in a low-temperature environment; meanwhile, a double-layer heat preservation cover and a composite heat insulation structure are adopted, heat exchange is effectively restrained, the heat preservation performance is improved, and liquid nitrogen consumption and sample storage risks are reduced.

The application discloses an embryo freezing dish and an embryo vitrification method, and belongs to the field of medical devices. The embryo freezing dish is provided with a receiving hole and at least two liquid storage pools. The receiving hole is used for accommodating embryos, and the liquid storage pools are used for containing vitrification solutions. The at least two liquid storage pools are respectively communicated with the receiving hole. A gland is installed on a dish body. A stand column extends into the dish body and is located at the upper end of the receiving hole. A micro flow channel is formed between the stand column and the receiving hole. The micro flow channel is communicated with the at least two liquid storage pools. Through the above design, different volumes of reagents are injected into the two liquid storage pools. The reagents are communicated through the micro flow channel but do not flow. The reagent on the side with a small volume is sucked. Under the liquid tension, the liquid on the other side will flow to the other side through the micro flow channel and be sucked. In the process, the vitrification reagent will pass through the receiving hole and can fully soak the embryo, realizing the washing effect on the embryo and achieving the vitrification treatment effect on the embryo. In the embryo vitrification and freezing operation process, the embryo does not need to be transferred from the freezing dish, so that the damage is reduced.

The utility model belongs to the technical field of embryo freezing devices, and provides an outer sleeve for storing an embryo freezing carrying rod, which comprises a sleeve main body, a first sleeve body, a second sleeve body, a third sleeve body, a fourth sleeve body, a fourth sleeve body and a fifth sleeve body, and is characterized in that the first sleeve body is used for storing the embryo freezing carrying rod; the second pipe body is hollow, and the first end is open; the limiting sleeve is hollow, the first end of the limiting sleeve is open, the limiting sleeve is arranged in the second pipe body, the limiting sleeve is provided with a first position and a second position which can be switched mutually, when the limiting sleeve is located at the first position, the limiting sleeve is located in the second pipe body, and when the limiting sleeve is located at the second position, the limiting sleeve is located in the second pipe body. The first end of the limiting sleeve extends into the first pipe body; the first side of the first end of the first pipe body is hinged to the first side of the first end of the second pipe body, and an operation opening is formed in the side wall of the second end of the second pipe body. The outer sleeve for storing the embryo freezing carrying rod is simple in structure, and the embryo freezing carrying rod is convenient to take and place.

The application discloses a kind of embryo freezing transfer liquid nitrogen tank and embryo freezing transfer method, belong to medical instrument field, transfer liquid nitrogen tank is provided with multiple sealing caps and bearing frame, multiple sealing caps can be detachably mounted in tank cover, bearing frame is installed in frozen space, the compartment is formed between the adjacent two partitions of bearing frame, the compartment is formed between partition and tank body, the number of compartment is same with the number of sealing cap, the position of sealing cap corresponds with the position of compartment, guide rod is located in compartment, guide rod is provided with push rod piece, push rod piece is in contact with vitrification assembly so that vitrification assembly falls into compartment, by the above design, when vitrification assembly is moved into embryo freezing transfer liquid nitrogen tank, only sealing cap needs to be opened, tank cover does not need to be opened;In addition, guide rod is located in compartment, the push rod piece of guide rod is in contact with vitrification assembly so that vitrification assembly falls into compartment, it is easy to operate, and compartment can store multiple vitrification assemblies, increase the number of vitrification assemblies stored in embryo freezing transfer liquid nitrogen tank.

The utility model relates to the technical field of ultra-low temperature cryopreservation, and discloses a high-flux semi-automatic oocyte and embryo freezing carrier which comprises a blade and a handle, the blade is connected with the handle, a freezing operation area and a bearing area are arranged on the blade, and the bearing area is connected with the blade. A reagent inflow area, a sample placement area and a reagent outflow area are arranged in the freezing operation area, a channel is arranged in the sample placement area, and a sample adding hole is formed in the channel. The volume of a cryoprotectant during oocyte / embryo freezing can be accurately controlled by setting the inner diameter and the height of the sample adding holes, so that the cooling speed can be quickly reduced, the technical difficulty of freezing operation can be reduced, the freezing effect is improved, and by arranging the three channels and the plurality of sample adding holes, the freezing efficiency is improved. According to the technical scheme, the number of single-time freezing of oocytes / embryos can be remarkably increased, the freezing flux can be improved, in addition, the freezing operation process is simplified by arranging the freezing operation area on the blade, and the consumption of consumables can be reduced.

The integrated carrier device comprises a microporous membrane substrate, a frame and a sealing film, a plurality of through holes are formed in the microporous membrane substrate, the frame is arranged on the edge of the microporous membrane substrate, and the sealing film is arranged at the top end of the frame. The device further comprises a base, and an adsorption block matched with the lower surface of the microporous membrane substrate is fixedly arranged on the upper surface of the base. According to the integrated carrier device for embryo freezing and unfreezing, by changing the structure of the freezing carrier, all key steps are completed on the carrier, embryo transfer is not needed, the operation difficulty and complexity are reduced, the operation steps are reduced, time is more easily and accurately controlled, and the probability that due to different proficiency degrees of an operator, the operation time is shortened is reduced. And effect differences caused by random errors of the operation process.

The utility model discloses an ovum or embryo freezing plate which comprises a freezing plate body, the freezing plate body comprises a first hole, a second hole and a liquid drop groove, and the liquid drop groove is adjacently arranged between the first hole and the second hole; the cover plate is connected with the freezing plate body in a buckled mode so as to cover the first hole, the second hole and the liquid drop groove. Compared with a traditional four-hole plate, the four-hole plate has the advantages that more plate surface space is directly released due to the design that hole sites are reduced, and a larger degree of freedom is provided for operation. More importantly, the liquid drop groove is ingeniously additionally arranged between the first hole and the second hole, the liquid drop groove not only expands the area for cleaning and replacing the cryoprotectant, but also enables an operator to treat a plurality of ova or embryos at the same time, and the working efficiency is greatly improved.

The culture dish comprises a culture dish body, a plurality of first storage holes are formed in the left side of the upper end of the culture dish body, a plurality of second storage holes are formed in the right side of the upper end of the culture dish body, the diameter of each first storage hole is larger than that of each second storage hole, and a containing groove is formed in the rear side of the upper end of the culture dish body. A first clamping groove extending to the outer side of the culture dish is formed in the left end of the placement groove, a second clamping groove extending to the outer side of the culture dish is formed in the right end of the placement groove, and the first clamping groove and the second clamping groove are parallel front and back. During use, after the freezing carrying rod is opened, the freezing carrying rod is clamped in the first clamping groove, the open end of the freezing carrying rod is located in the containing groove, the sleeve is clamped in the second clamping groove, and the open end of the freezing carrying rod is located in the containing groove, so that the freezing carrying rod is convenient to store, and pollution is avoided.

The application provides a pretreatment liquid for bovine in-vitro embryo programmed freezing and a programmed freezing method, and the pretreatment liquid comprises an embryo culture liquid and 2-10 mu g / L cytochalasin B and 0.2-1.5 mM 4-phenylbutyric acid. The freezing liquid comprises the following components: 0.05-0.25 mg / mL hyaluronic acid, 2-20 uM astaxanthin, 0.01-0.12 mg / mL L-carnitine, 33.8-34.5 mg / mL sucrose, 0.3-2.0 mg / mL glucose, 5-20 %v / v ethylene glycol, 3-15 mg / mL bovine serum albumin and 25-45 mg / L sodium pyruvate PBS solution. The application comprehensively solves the in-vitro embryo freezing damage repair problem from two aspects of before freezing and during freezing, so that the in-vitro embryo is frozen by using the programmed freezing mode, and ideal thawing recovery rate and pregnancy rate are achieved, the industry problem is solved, and the development and application of the in-vitro embryo production technology are promoted.