29 results about "Embryo freezing" patented technology

The utility model provides an embryo freezing operation box which comprises a spliced box body, a metal inner container arranged in the box body and a top cover installed at the upper end of the box body, the box body comprises a bottom layer die holder and a top layer module, and a plurality of sets of middle modules are arranged between the bottom layer die holder and the top layer module in a stacked mode. The height of the box body is adjusted according to the metal liner; a plurality of magnetic connecting columns are distributed on the attaching end faces among the bottom-layer die base, the middle die block and the top-layer die block so that the stability of mutual connection can be improved. The top cover at the upper end of the box body adopts a partial opening design and can be covered during operation, so that the air contact area is greatly reduced, and the liquid nitrogen consumption speed is greatly reduced; and a liquid level sensor is mounted on the top cover. The lighting assembly is arranged at the top of the box body and detachably installed on the top layer module through the magnetic connecting column, a person can adjust the height and the angle of the lamp holder through the adjusting piece so that a light source can irradiate into the metal inner container, and observation and operation of the person are facilitated.

This invention provides a method for the cryopreservation and reconstitution of porcine embryos. During the in vitro maturation of porcine oocytes, porcine IVF embryo culture, and embryo freezing and thawing processes, the addition of TSA, 5-Aza-dC, Etomoxir, GSK-J4, and NR, either alone or in combination, improves the development and freezing efficiency of porcine IVF embryos. Experiments show that the oocyte maturation rate, embryo cleavage rate, and blastocyst rate of the groups adding TSA, 5-Aza-dC, and Etomoxir to the porcine oocyte in vitro maturation culture medium and porcine IVF embryo culture medium are significantly higher than those of the control group. Furthermore, the hatching rate of porcine IVF embryos after freezing and thawing is significantly higher in the groups adding GSK-J4 and NR to the freezing and thawing solutions than in the control group. This invention lays the foundation for the production of porcine in vitro fertilized embryos and related scientific research, providing an effective, safe, and feasible strategy for improving the production efficiency and quality of porcine in vitro embryos.

The embryo freezing and transferring device for the reproduction laboratory comprises a shell and a base, a buffering mechanism used for buffering a heat preservation box is arranged in the shell, the buffering mechanism comprises a square groove slidably connected with the inner wall of the shell, a two-way threaded rod is rotationally connected into the square groove, and the two-way threaded rod is in threaded connection with a first moving plate; according to the invention, the vibration and impact resistance is effectively improved, the embryo injury risk is reduced, the buffer structure design is optimized, for example, a plurality of groups of elastic buffer assemblies are adopted to replace a traditional simple filling material, so that energy generated by transportation jolt can be absorbed in a targeted manner; the collision between the embryo freezing tube and the inner wall of the box body is reduced, the pollution hidden danger is reduced, and the sterility in the transfer process is improved. The split type detachable structural design is adopted, cleaning dead corners of the integrated box body can be eliminated, and the inner box body and the outer box body can be thoroughly cleaned and disinfected conveniently.

The utility model aims to provide an embryo freezing and transferring device for a reproductive laboratory, and relates to the technical field of medical instruments, the upper end of a freezing bracket is clamped on a cross rod of a sealing cover, the freezing bracket is placed in a storage cylinder of a storage assembly, a shielding cylinder is rotated and a through hole of the storage cylinder is opened while the sealing cover is rotated and tightened, and the freezing bracket is placed in the storage assembly. Liquid nitrogen in the box body can flow into the storage cylinder through the through hole; the sealing cover is opened through rotation, the sealing cover is held by hand to take out the freezing support clamped on the cross rod of the sealing cover out of the storage barrel, and the shielding barrel is reversed to block the through hole in the storage barrel and prevent liquid nitrogen from flowing into the storage barrel while the sealing cover is opened through rotation, so that the situation that the temperature in the box body rises too fast due to continuous overflow of the liquid nitrogen, waste is caused is prevented; when the temperature in the box body is too high, the control module automatically controls the liquid nitrogen electromagnetic valve to be opened to supplement liquid nitrogen for the box body, and the low-temperature environment of frozen embryos is guaranteed.

The liquid nitrogen box comprises a liquid nitrogen box body, the liquid nitrogen box body comprises an inner freezing heat preservation cavity and an outer protection shell, a first hole frame used for containing a freezing pipe is fixedly arranged at the bottom of the inner side of the inner freezing heat preservation cavity, and a second supporting assembly used for supporting the upper portion of the freezing pipe is arranged on the upper portion of the first hole frame. The second supporting assembly is connected with an adjusting assembly on the side wall of the inner freezing heat preservation cavity so as to adjust the vertical height of the second supporting assembly relative to the first hole frame, and a double-layer heat preservation cover is arranged in the liquid nitrogen box body and comprises an outer cover arranged on the upper portion of the liquid nitrogen box body and an inner heat preservation cover in threaded connection with the inner freezing heat preservation cavity. According to the utility model, the second supporting assembly with adjustable height is arranged, so that the liquid nitrogen box can accommodate embryo freezing tubes with different lengths, and a sample is ensured to be completely immersed in a low-temperature environment; meanwhile, a double-layer heat preservation cover and a composite heat insulation structure are adopted, heat exchange is effectively restrained, the heat preservation performance is improved, and liquid nitrogen consumption and sample storage risks are reduced.

The utility model relates to the technical field of freezing carrying rods, in particular to a telescopic embryo freezing carrying rod which comprises a freezing blade, one end of the freezing blade is connected with a connecting plate, one end of the connecting plate is provided with a storage box, a telescopic rod is elastically arranged in the storage box in a sliding mode, and the extending end of the telescopic rod is connected with the connecting plate. A telescopic rod is installed in the containing box, a telescopic sleeve is arranged outside the containing box in a sleeving mode, the telescopic sleeve slides towards the end away from the telescopic rod so as to drive the telescopic rod to slide out of the containing box, a partition plate is installed in the containing box, and the interior of the containing box is divided into a containing cavity and a driving cavity through the partition plate. According to the freezing carrying rod, the telescopic sleeve slides backwards, the gear disc is matched with the gear block, the handheld part can be unfolded, the telescopic sleeve is loosened, the elastic force of the spring is utilized, the handheld part can be automatically stored, the freezing carrying rod which is easy to operate and control is achieved, and the using convenience and practicability of the freezing carrying rod are improved.

The application discloses an embryo freezing dish and an embryo vitrification method, and belongs to the field of medical devices. The embryo freezing dish is provided with a receiving hole and at least two liquid storage pools. The receiving hole is used for accommodating embryos, and the liquid storage pools are used for containing vitrification solutions. The at least two liquid storage pools are respectively communicated with the receiving hole. A gland is installed on a dish body. A stand column extends into the dish body and is located at the upper end of the receiving hole. A micro flow channel is formed between the stand column and the receiving hole. The micro flow channel is communicated with the at least two liquid storage pools. Through the above design, different volumes of reagents are injected into the two liquid storage pools. The reagents are communicated through the micro flow channel but do not flow. The reagent on the side with a small volume is sucked. Under the liquid tension, the liquid on the other side will flow to the other side through the micro flow channel and be sucked. In the process, the vitrification reagent will pass through the receiving hole and can fully soak the embryo, realizing the washing effect on the embryo and achieving the vitrification treatment effect on the embryo. In the embryo vitrification and freezing operation process, the embryo does not need to be transferred from the freezing dish, so that the damage is reduced.

The invention discloses an embryo freezing resuscitation reagent and a resuscitation method, and belongs to the technical field of embryo freezing resuscitation. According to the embryo freezing resuscitation reagent provided by the invention, resuscitation liquid is improved, and compared with an existing 1M sucrose solution, the same resuscitation effect can be achieved only by adopting a 0.3-035 M sucrose solution. The reagent concentration is remarkably reduced, and the cost can be effectively reduced. The invention further provides a rapid resuscitation method matched with the embryo freezing resuscitation reagent, the whole resuscitation process of one embryo is about 2 min, 20 FET periods can be completed only about 40 min, the working efficiency is greatly improved, the operation process is simplified, the waiting time of a patient is shortened, and the satisfaction degree of the patient is improved. The embodiment proves that the rapid resuscitation scheme has no influence on the survival of the blastocyst, the survival rate of the resuscitated blastocyst reaches 100%, the continuous pregnancy rate can be effectively increased, and the abortion rate is reduced.

The utility model discloses an embryo freezing carrier support which comprises an L-shaped rear support body, the outer wall of the L-shaped rear support body is fixedly connected with a first side plate, two rectangular sliding grooves are formed in the outer wall of the L-shaped rear support body, the inner walls of the two rectangular sliding grooves are connected with rectangular blocks in a sliding mode, the outer walls of the rectangular blocks are fixedly connected with rectangular support bodies, and the rectangular support bodies are fixedly connected with the first side plate. One ends of the two rectangular supports are fixedly connected with the same second side plate, the outer wall of the first side plate is evenly and fixedly connected with a plurality of placing frames, rod carrying boxes are placed on the tops of the placing frames, and cross-shaped partition plates are fixedly connected into the rod carrying boxes. By arranging the L-shaped rear support, the first side plate, the rectangular block, the rectangular support, the placement frame, the carrying rod box, the cross-shaped partition plate, the box cover, the locking bolt and the label groove, the frozen carrying rod can be conveniently placed and protected, and a marked label is bonded through the label groove so that subsequent taking can be facilitated.

The present invention discloses an additive for freezing liquid in a one-step method for bovine in vitro embryos and a one-step freezing method for bovine in vitro embryos, and belongs to the technical field of animal embryo cryopreservation and embryo transplantation. The additive of the present invention is an aqueous solution comprising sodium chloride, potassium chloride, magnesium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate, glucose, sodium pyruvate, calcium chloride, bovine serum albumin, sucrose, ethylene glycol and hyaluronic acid; the concentration of sucrose is 4.5 to 5.0 M. Based on the one-step freezing method for bovine in vitro embryos, the present invention increases the sucrose concentration in the conventional one-step freezing liquid and additionally adds hyaluronic acid, thereby increasing the osmotic pressure of the freezing liquid, facilitating the discharge of water inside the embryo, reducing the formation of ice crystals inside the embryo during the freezing process, reducing the damage to the embryo during the freezing process, and improving the survival rate, hatching rate and transplantation pregnancy rate of bovine in vitro embryos after freezing and thawing.

The invention relates to a freezing carrier device capable of absorbing embryo freezing fluid and application of the freezing carrier device. The freezing carrier device comprises a freezing pipe body, fluid-absorbing resin particles are adhered to the inner wall of the freezing pipe body through a polyurethane adhesive, tiny gaps exist between the fluid-absorbing resin particles, numerous micro-channels are formed, and a capillary effect is generated on the fluid. The liquid-absorbing resin particles used for absorbing the liquid can be polyacrylamide, sodium polyacrylate or a mixture of the polyacrylamide and the sodium polyacrylate. When the freezing carrier device is used, an embryo and a protective agent are mixed and then put into the freezing pipe body, and due to the liquid absorption performance (the hydrophilic effect of resin and the capillary effect of particle gaps) of the liquid absorption resin particle layer, the liquid absorption resin particle layer can rapidly absorb redundant embryo freezing liquid in embryo freezing liquid, so that the volume of the embryo freezing liquid is reduced, and the embryo freezing effect is improved. The freezing and unfreezing speed is increased, the damage of ice crystals to embryos is reduced, and the survival rate and conception rate of the embryos are increased.

The utility model belongs to the technical field of embryo freezing devices, and provides an outer sleeve for storing an embryo freezing carrying rod, which comprises a sleeve main body, a first sleeve body, a second sleeve body, a third sleeve body, a fourth sleeve body, a fourth sleeve body and a fifth sleeve body, and is characterized in that the first sleeve body is used for storing the embryo freezing carrying rod; the second pipe body is hollow, and the first end is open; the limiting sleeve is hollow, the first end of the limiting sleeve is open, the limiting sleeve is arranged in the second pipe body, the limiting sleeve is provided with a first position and a second position which can be switched mutually, when the limiting sleeve is located at the first position, the limiting sleeve is located in the second pipe body, and when the limiting sleeve is located at the second position, the limiting sleeve is located in the second pipe body. The first end of the limiting sleeve extends into the first pipe body; the first side of the first end of the first pipe body is hinged to the first side of the first end of the second pipe body, and an operation opening is formed in the side wall of the second end of the second pipe body. The outer sleeve for storing the embryo freezing carrying rod is simple in structure, and the embryo freezing carrying rod is convenient to take and place.

The utility model relates to the field of animal husbandry breeding auxiliary instruments, and discloses a cattle and sheep embryo freezing instrument which comprises a protection box, the top of the protection box is connected with a protection ring, and a mounting cover is clamped on the protection ring; one end of the thermal insulation column is located on the outer side of the protection ring, the other end of the thermal insulation column is connected with a placement mechanism located in the protection box, the placement mechanism comprises a sleeving ring, one side of the sleeving ring is connected with one end of the thermal insulation column, the middle of the sleeving ring is in threaded sleeving connection with a mounting ring, and the inner side of the mounting ring is fixedly connected with a supporting block; according to the embryo storage vessel, the storage vessel can be conveniently placed, meanwhile, suspended supporting, internal data detection and taking can be carried out conveniently, meanwhile, temperature dissipation can be greatly reduced, possible frostbite can be reduced, internal storage stability is guaranteed, and embryo stable storage is guaranteed; and meanwhile, personnel monitoring is reduced, and the overall workload is reduced.

The invention belongs to the technical field of embryo freezing, and particularly discloses a freezing carrier device for embryo cryopreservation, which comprises a handheld part and a freezing part connected to one end of the handheld part, the freezing part is in a groove shape, and a first polymer hydrogel coating, a second polymer hydrogel coating and a third polymer hydrogel coating are sequentially coated in the groove of the freezing part; the high-molecular hydrogel coating has good liquid absorption capacity and can rapidly absorb redundant refrigerating fluid in the embryo refrigerating fluid, so that the volume of the embryo refrigerating fluid is reduced, the freezing and unfreezing speeds are increased, the damage of ice crystals to embryos is reduced, and the survival rate and conception rate of the embryos are increased.

The application discloses a kind of embryo freezing transfer liquid nitrogen tank and embryo freezing transfer method, belong to medical instrument field, transfer liquid nitrogen tank is provided with multiple sealing caps and bearing frame, multiple sealing caps can be detachably mounted in tank cover, bearing frame is installed in frozen space, the compartment is formed between the adjacent two partitions of bearing frame, the compartment is formed between partition and tank body, the number of compartment is same with the number of sealing cap, the position of sealing cap corresponds with the position of compartment, guide rod is located in compartment, guide rod is provided with push rod piece, push rod piece is in contact with vitrification assembly so that vitrification assembly falls into compartment, by the above design, when vitrification assembly is moved into embryo freezing transfer liquid nitrogen tank, only sealing cap needs to be opened, tank cover does not need to be opened;In addition, guide rod is located in compartment, the push rod piece of guide rod is in contact with vitrification assembly so that vitrification assembly falls into compartment, it is easy to operate, and compartment can store multiple vitrification assemblies, increase the number of vitrification assemblies stored in embryo freezing transfer liquid nitrogen tank.

The utility model relates to a sheep embryo freezing device, which comprises a box body, a bracket, a box cover and freezing tubes, the bracket is arranged in the box body, sunken grooves are uniformly distributed on the bracket, and the freezing tubes are arranged in the grooves; a box cover is arranged at the top of the box body; a cavity between the bracket and the box body is filled with a freezing medium; and the bracket is also provided with a liquid nitrogen supplementing device and a temperature detection probe. On the basis of a medium cryopreservation box, a device for detecting the temperature of the cryopreservation box and a corresponding device for supplementing liquid nitrogen to a medium to accelerate cooling of the medium are additionally arranged, and by monitoring the temperature of the cryopreservation box, the medium in the cryopreservation box is accelerated to cool in a short time in a liquid nitrogen supplementing mode when the temperature is reduced to a set low temperature; therefore, the retention time of the ice crystals in an ice crystal generating zone is reduced, and the generation of the ice crystals is reduced, namely the mechanical damage of the ice crystals to embryonic cells is reduced.

The invention provides an optimization method of early embryo vitrification refrigerating fluid, and belongs to the technical field of early embryo vitrification refrigerating fluid. Multi-dimensional evaluation data such as the embryo survival rate of a multi-component cryoprotectant formula are collected by establishing an orthogonal experimental design; a comprehensive feature vector is formed through weighted fusion, the survival rate and the cost effectiveness are balanced in combination with a double-layer game model, the comprehensive feature is input into an embryo freezing effect prediction model, and model parameters are dynamically adjusted by adopting a formula complexity evaluation function and a gating weight function to obtain a formula optimization vector; and according to the optimization vector, adjusting the concentration of the formula components to generate a candidate formula, performing experimental verification, and determining a final optimization formula through iterative optimization until the improvement amplitude of the comprehensive evaluation index is smaller than a threshold value, so that the technical problem that the formula optimization of the multi-component cryoprotectant lacks a systematic prediction model and an accurate regulation and control method is solved.

The utility model relates to the technical field of ultra-low temperature cryopreservation, and discloses a high-flux semi-automatic oocyte and embryo freezing carrier which comprises a blade and a handle, the blade is connected with the handle, a freezing operation area and a bearing area are arranged on the blade, and the bearing area is connected with the blade. A reagent inflow area, a sample placement area and a reagent outflow area are arranged in the freezing operation area, a channel is arranged in the sample placement area, and a sample adding hole is formed in the channel. The volume of a cryoprotectant during oocyte / embryo freezing can be accurately controlled by setting the inner diameter and the height of the sample adding holes, so that the cooling speed can be quickly reduced, the technical difficulty of freezing operation can be reduced, the freezing effect is improved, and by arranging the three channels and the plurality of sample adding holes, the freezing efficiency is improved. According to the technical scheme, the number of single-time freezing of oocytes / embryos can be remarkably increased, the freezing flux can be improved, in addition, the freezing operation process is simplified by arranging the freezing operation area on the blade, and the consumption of consumables can be reduced.

The utility model belongs to the technical field of freezing tubes, and discloses an embryo freezing tube which comprises a storage tube, a fixing cover is arranged at the top of the storage tube, a carrying rod is arranged in the storage tube, through the design of a stabilizing mechanism, the carrying rod can be fixed conveniently, and therefore the stability of embryo transportation is guaranteed, knobs at one ends of two pulling rods are pulled, and the embryo freezing tube is formed. The clamping blocks move towards the two sides, the limiting springs are compressed, the carrying rod is placed in the storage pipe and located between the two stabilizing mechanisms, a rotary knob is slowly loosened, under the action of the limiting springs, the clamping blocks move towards the direction of the carrying rod, the carrying rod is clamped, stability of the carrying rod is guaranteed, embryos are placed on the carrying rod, and in the transportation and storage process, the embryos can be subjected to various kinds of shaking and vibration, and the stability of the embryos is guaranteed. The loading rod is clamped by the stabilizing mechanism, so that the loading rod can be prevented from moving in the storage pipe, the embryo is ensured to be always located at a relatively fixed position, and the embryo is prevented from being mechanically damaged due to shaking of the loading rod.

The invention provides an embryo freezing and unfreezing machine. The embryo freezing and unfreezing machine comprises a rack, a supporting seat and a driving device, the perforated plate can be supported on the rack, the supporting seat is movably arranged on the rack, the perforated plate is placed at the upper end of the rack, the supporting seat is close to the bottom of the perforated plate from bottom to top, then is in butt joint with the lower end of the perforated plate and heats the bottom of a hole grid, containing unfreezing liquid, of the perforated plate, and the driving device is used for driving the supporting seat to move. In the embodiment of the utility model, one hole grid of the perforated plate is heated through the supporting seat, so that the thawing liquid, the diluent, the No.1 flushing liquid and the No.2 flushing liquid are simultaneously positioned on the same perforated plate, and an embryo is transferred on the same perforated plate during embryo thawing and is prevented from falling out of the perforated plate.

The invention relates to the technical field of molecular biological detection, in particular to a method for rapidly detecting chromosome aneuploidy before embryo implantation based on a CRISPR-Cas12a (clustered regularly interspaced short palindromic repeats-associated 12a) system. The method comprises the following steps: acquiring a biopsy cell sample, processing the sample to release DNA, preparing a CRISPR-Cas12a detection reaction system, adding the sample DNA and carrying out a reaction, detecting fluorescence signal change, calculating a fluorescence signal ratio of a to-be-detected chromosome to an internal reference chromosome, finally judging a chromosome copy number state according to the signal ratio, and obtaining a result from the sample and outputting the result. The method only needs 2-4 hours, so that aneuploid screening can be completed before embryo transplantation when a fresh embryo transplantation strategy is adopted, the potential influence of embryo freeze thawing on quality is reduced, a common fluorescence detector instead of an expensive high-throughput sequencer or a microarray scanner is used, and the single sample detection cost is reduced by 50-70%.

The invention discloses a medical human embryo freezing device which comprises an information processing assembly, a storage assembly, a carrying rod and a carrying rod sleeve. A storage assembly is arranged on one side of the information processing assembly; a carrying rod is arranged in the storage assembly; the outer part of the carrying rod is in threaded connection with a carrying rod sleeve; the carrying rod comprises a carrying sheet, a positioning head, a fixed external thread, a limiting sleeve, an indicator, a positioning ring, a magnet and a label plate; the upper side of the front end of the slide is provided with a positioning head, and the rear end is provided with a fixed external thread movably connected with a carrying rod sleeve; a limiting sleeve is arranged at the rear end of the fixed external thread; a label plate is arranged at the rear end of the limiting sleeve; the lower part of the rear end of the label plate is provided with a magnet; the tail end of the label plate is provided with a positioning ring which is movably connected with the storage assembly; the direction of the carrying rod can be quickly determined through the positioning head, so that the operation efficiency is greatly improved, the embryo transfer deviation caused by wrong direction judgment is reduced, and a guarantee is provided for safe transfer of embryos.

The integrated carrier device comprises a microporous membrane substrate, a frame and a sealing film, a plurality of through holes are formed in the microporous membrane substrate, the frame is arranged on the edge of the microporous membrane substrate, and the sealing film is arranged at the top end of the frame. The device further comprises a base, and an adsorption block matched with the lower surface of the microporous membrane substrate is fixedly arranged on the upper surface of the base. According to the integrated carrier device for embryo freezing and unfreezing, by changing the structure of the freezing carrier, all key steps are completed on the carrier, embryo transfer is not needed, the operation difficulty and complexity are reduced, the operation steps are reduced, time is more easily and accurately controlled, and the probability that due to different proficiency degrees of an operator, the operation time is shortened is reduced. And effect differences caused by random errors of the operation process.

The invention discloses an automatic embryo vitrification freezing workstation, relates to the technical field of embryo freezing, and aims to solve the technical problem that in the prior art, a carrying rod is in direct contact with liquid nitrogen, so that the liquid nitrogen generates bubbles, and the success rate of embryo freezing is affected. Comprising a cabinet body, an automatic production line installed on the cabinet body, a carrying rod located on the automatic production line and a freezing assembly arranged on the cabinet body. Through the design of the freezing assembly, the heat conduction protection rod serves as an intermediate conduction medium of liquid nitrogen, the carrying rod is completely separated from direct contact with the liquid nitrogen, cooling capacity is transmitted through the efficient heat conduction characteristic of the protection rod, boiling bubbles caused by direct contact with the liquid nitrogen are avoided, bubble interference is eliminated, and the precision of subsequent embryo freezing state detection is improved. Through the design of the freezing assembly, direct contact between liquid nitrogen and the carrying rod is avoided, so that the freezing and detection process of the carrying rod is optimized, the success rate of embryo vitrification is increased, and the cell viability retention rate after resuscitation is increased.

The utility model relates to the technical field of embryo freezing dishes, and discloses a biopsy embryo freezing dish which comprises a freezing dish bottom, eight embryo storage holes are formed in the freezing dish bottom, eight cleaning holes are formed in the freezing dish bottom, and a cleaning hole is correspondingly formed beside each embryo storage hole; two guide grooves are symmetrically formed in the freezing dish bottom, sliding grooves are formed below the guide grooves, the guide grooves communicate with the sliding grooves, a sealing protrusion is arranged on the freezing dish bottom, the freezing dish cover is installed on the freezing dish bottom in a nested mode, an annular groove is formed in the freezing dish cover, and two guide blocks are symmetrically installed on the freezing dish cover. The groove design is arranged at the dish bottom of the freezing dish, biopsy embryos are not easy to swing out of liquid to be mixed with embryos in other liquid drops in the freezing process, the locking mechanism is arranged to prevent the freezing dish from being opened by mistake, embryo serial numbers are set for the embryo storage holes to distinguish different embryos, and the operation is convenient. A freezing vessel serial number is set for each freezing vessel so as to facilitate management and query.

The utility model discloses an ovum or embryo freezing plate which comprises a freezing plate body, the freezing plate body comprises a first hole, a second hole and a liquid drop groove, and the liquid drop groove is adjacently arranged between the first hole and the second hole; the cover plate is connected with the freezing plate body in a buckled mode so as to cover the first hole, the second hole and the liquid drop groove. Compared with a traditional four-hole plate, the four-hole plate has the advantages that more plate surface space is directly released due to the design that hole sites are reduced, and a larger degree of freedom is provided for operation. More importantly, the liquid drop groove is ingeniously additionally arranged between the first hole and the second hole, the liquid drop groove not only expands the area for cleaning and replacing the cryoprotectant, but also enables an operator to treat a plurality of ova or embryos at the same time, and the working efficiency is greatly improved.

The culture dish comprises a culture dish body, a plurality of first storage holes are formed in the left side of the upper end of the culture dish body, a plurality of second storage holes are formed in the right side of the upper end of the culture dish body, the diameter of each first storage hole is larger than that of each second storage hole, and a containing groove is formed in the rear side of the upper end of the culture dish body. A first clamping groove extending to the outer side of the culture dish is formed in the left end of the placement groove, a second clamping groove extending to the outer side of the culture dish is formed in the right end of the placement groove, and the first clamping groove and the second clamping groove are parallel front and back. During use, after the freezing carrying rod is opened, the freezing carrying rod is clamped in the first clamping groove, the open end of the freezing carrying rod is located in the containing groove, the sleeve is clamped in the second clamping groove, and the open end of the freezing carrying rod is located in the containing groove, so that the freezing carrying rod is convenient to store, and pollution is avoided.

The application provides a pretreatment liquid for bovine in-vitro embryo programmed freezing and a programmed freezing method, and the pretreatment liquid comprises an embryo culture liquid and 2-10 mu g / L cytochalasin B and 0.2-1.5 mM 4-phenylbutyric acid. The freezing liquid comprises the following components: 0.05-0.25 mg / mL hyaluronic acid, 2-20 uM astaxanthin, 0.01-0.12 mg / mL L-carnitine, 33.8-34.5 mg / mL sucrose, 0.3-2.0 mg / mL glucose, 5-20 %v / v ethylene glycol, 3-15 mg / mL bovine serum albumin and 25-45 mg / L sodium pyruvate PBS solution. The application comprehensively solves the in-vitro embryo freezing damage repair problem from two aspects of before freezing and during freezing, so that the in-vitro embryo is frozen by using the programmed freezing mode, and ideal thawing recovery rate and pregnancy rate are achieved, the industry problem is solved, and the development and application of the in-vitro embryo production technology are promoted.

The present application belongs to the technical field of chemical delivery. In order to solve the problems of difficult operation and poor stability in the process of chemical delivery in the process of oocyte / embryo freezing treatment by using the existing method, the present application discloses a chemical delivery system and method, wherein the system comprises a carrier and a chip; the carrier is provided with a groove for containing a solution; the chip is provided with an interval and the chemical to be delivered is arranged in the interval, and the coverage area of the interval is greater than the opening area of the groove; the chip and the carrier can move relative to each other, the chemical to be delivered in the interval can fully cover and contact the solution in the groove, and a free diffusion is formed between them. By the present application, the difficulty of the operation can be reduced, and the stability and reliability of the operation process can be improved in the process of chemical delivery in the process of oocyte / embryo freezing treatment.