Embryo freezing transfer liquid nitrogen tank and embryo freezing transfer method

Abstract

The application discloses a kind of embryo freezing transfer liquid nitrogen tank and embryo freezing transfer method, belong to medical instrument field, transfer liquid nitrogen tank is provided with multiple sealing caps and bearing frame, multiple sealing caps can be detachably mounted in tank cover, bearing frame is installed in frozen space, the compartment is formed between the adjacent two partitions of bearing frame, the compartment is formed between partition and tank body, the number of compartment is same with the number of sealing cap, the position of sealing cap corresponds with the position of compartment, guide rod is located in compartment, guide rod is provided with push rod piece, push rod piece is in contact with vitrification assembly so that vitrification assembly falls into compartment, by the above design, when vitrification assembly is moved into embryo freezing transfer liquid nitrogen tank, only sealing cap needs to be opened, tank cover does not need to be opened;In addition, guide rod is located in compartment, the push rod piece of guide rod is in contact with vitrification assembly so that vitrification assembly falls into compartment, it is easy to operate, and compartment can store multiple vitrification assemblies, increase the number of vitrification assemblies stored in embryo freezing transfer liquid nitrogen tank.

Description

Technical Field

[0001] This invention relates to the field of medical devices, and in particular to a liquid nitrogen tank for embryo cryopreservation and a method for embryo cryopreservation. Background Technology

[0002] Embryo vitrification involves using a high concentration of cryoprotectant to rapidly displace the water within the embryo, followed by rapid cooling in liquid nitrogen at -196°C to vitrify the internal liquid, thus reducing damage from ice crystals. Finally, the embryo is stored in a liquid nitrogen tank for long-term preservation and thawed for later use. Vitrification typically utilizes a transfer liquid nitrogen tank, which includes a tank body, a lid, and a support for holding the vitrification carrier. Before use, a certain amount of liquid nitrogen is added to the tank, and the support is placed inside for pre-cooling. After vitrification, the carrier is placed in the support, completing the embryo vitrification process. However, the lid of a traditional transfer liquid nitrogen tank remains open during the freezing process, causing rapid evaporation of liquid nitrogen and reducing the storage time. Furthermore, the low insulation of traditional transfer liquid nitrogen tanks also exacerbates evaporation. This increases the risk of carriers being exposed to air during transfer to the storage tank and limits the number of carriers that can be frozen simultaneously in a single tank. Summary of the Invention

[0003] In order to overcome the shortcomings of the prior art, one of the objectives of the present invention is to provide a liquid nitrogen transfer tank for embryo freezing that does not require the lid to be kept open during the freezing process and can freeze a large number of carrier rods at the same time.

[0004] In order to overcome the shortcomings of the prior art, the second objective of this invention is to provide a method for transferring frozen embryos during the freezing process in which the lid does not need to be kept open at all times and a single container can freeze a large number of carrier rods at the same time.

[0005] One of the objectives of this invention is achieved through the following technical solution:

[0006] An embryo cryopreservation liquid nitrogen tank includes a tank body and a tank lid. The tank lid is installed on the tank body, and a freezing space is formed between the tank body and the tank lid. The embryo cryopreservation liquid nitrogen tank also includes a sealing cover and a support frame. There are multiple sealing covers, which are detachably installed on the tank lid. The support frame is installed in the freezing space. The support frame includes a connecting rod, multiple partitions fixed to the connecting rod, and multiple guide rods fixed to the connecting rod. A compartment is formed between two adjacent partitions, and a compartment is formed between the partitions and the tank body. The number of compartments is the same as the number of sealing covers. The position of the sealing cover corresponds to the position of the compartment. The guide rod is located in the compartment, and the guide rod is provided with a pusher plate. The pusher plate abuts against a vitrification component, causing the vitrification component to fall into the compartment.

[0007] Furthermore, each compartment contains two guide rods, each guide rod has a guide surface, the guide surface is inclined, and a slide is formed between the two guide rods.

[0008] Furthermore, the length of the slide is less than the length of the compartment, the guide surface extends to the end of the guide rod, and the vitrified assembly flips within the slide and slides horizontally into the compartment.

[0009] Furthermore, the length of the slide is equal to the length of the compartment, the guide rod is also provided with a limiting protrusion, the limiting protrusion is located at the end of the guide surface, and the vitrification components are vertically arranged in the slide.

[0010] Furthermore, the can lid is provided with multiple inlets and at least one transfer area. The number of inlets is the same as the number of sealing caps. Each sealing cap is installed at one of the inlets. The transfer area is used to temporarily place the sealing cap.

[0011] Furthermore, the can lid includes an upper cover, a first insulation layer, and a cold insulation layer, wherein the first insulation layer is located between the upper cover and the cold insulation layer, and the transfer area is disposed on the upper surface of the upper cover.

[0012] Furthermore, the liquid nitrogen tank for embryo freezing transfer also includes a locking assembly, which includes a locking hook, a button, and a second elastic element. The locking hook is rotatably mounted on the tank lid, the button is slidably mounted on the tank lid and abuts against the locking hook, and the two ends of the second elastic element abut against the locking hook and the tank lid, respectively. The locking hook is snapped into the tank body.

[0013] Furthermore, the tank includes an outer liner and an inner liner, the inner liner being located inside the outer liner, and the support frame being installed inside the inner liner.

[0014] Furthermore, the sealing cover includes a cover body, an operating part, and a second heat insulation layer. The operating part extends from the upper surface of the cover body, and the second heat insulation layer extends from the lower surface of the cover body.

[0015] The second objective of this invention is achieved by the following technical solution:

[0016] An embryo cryopreservation transfer method, implemented using the aforementioned liquid nitrogen tank for embryo cryopreservation transfer, is characterized by comprising the following steps:

[0017] Liquid nitrogen is injected into the liquid nitrogen tank for embryo freezing and the amount of liquid nitrogen stored in the tank is monitored by a weighing sensor.

[0018] Move a sealing cap from the can lid inlet to the transfer area, so that the inlet corresponding to the compartment to be placed into the vitrification component is opened;

[0019] The cryogenic robotic arm clamps the vitrified assembly into the compartment;

[0020] The vitrified component comes into contact with the support frame to detach from the cryogenic robotic arm and falls into the compartment;

[0021] The vitrification assembly containing the embryo is vitrified in the liquid nitrogen tank of the embryo cryopreservation transfer station;

[0022] After vitrification is completed, the vitrified components are transferred one by one from the intermediate liquid nitrogen tank to the bucket using tweezers, and then the bucket is placed into the storage tank.

[0023] Compared to existing technologies, the liquid nitrogen tank for embryo cryopreservation in this invention features multiple sealing caps and a support frame. The sealing caps are detachably mounted on the tank lid, and the support frame is installed in the cryopreservation space. The support frame includes connecting rods, multiple partitions fixed to the connecting rods, and multiple guide rods fixed to the connecting rods. A compartment is formed between adjacent partitions, and a compartment is formed between the partitions and the tank body. The number of compartments is the same as the number of sealing caps, and the positions of the sealing caps correspond to the positions of the compartments. The guide rods are located within the compartments and are equipped with pusher plates. The pusher plates abut against the vitrified components, causing them to fall into the compartments. With this design, when the vitrified components are moved into the liquid nitrogen tank for embryo cryopreservation, only the sealing cap needs to be opened; the tank lid does not need to be opened. Furthermore, the guide rods are located within the compartments, and the pusher plates of the guide rods abut against the vitrified components, causing them to fall into the compartments. This facilitates operation, and the compartments can store multiple vitrified components, increasing the number of vitrified components that can be stored in the liquid nitrogen tank for embryo cryopreservation. Attached Figure Description

[0024] Figure 1 This is a perspective view of the liquid nitrogen tank for transferring frozen embryos according to the present invention;

[0025] Figure 2 for Figure 1 An exploded view of the liquid nitrogen tank used for transferring frozen embryos;

[0026] Figure 3 for Figure 1 A cross-sectional view of the liquid nitrogen tank used for transferring frozen embryos;

[0027] Figure 4 for Figure 3 A magnified view of point A in the liquid nitrogen transfer tank for embryo cryopreservation;

[0028] Figure 5 for Figure 2 A three-dimensional sectional view of the liquid nitrogen tank for transferring frozen embryos;

[0029] Figure 6 for Figure 2 A three-dimensional view of the sealed lid of the liquid nitrogen transfer tank for embryo cryopreservation;

[0030] Figure 7 for Figure 2 A perspective view of the support frame for the liquid nitrogen transfer tank for embryo cryopreservation in the first embodiment;

[0031] Figure 8 for Figure 7 A schematic diagram illustrating the use of the support frame;

[0032] Figure 9 for Figure 2 A perspective view of the support frame for the liquid nitrogen transfer tank for embryo cryopreservation, according to a second embodiment;

[0033] Figure 10 for Figure 9 A schematic diagram illustrating the use of the support frame;

[0034] Figure 11 This is a schematic diagram illustrating the process of using the liquid nitrogen tank for embryo freezing and transfer according to the present invention.

[0035] In the diagram: 80. Liquid nitrogen tank for embryo freezing transfer; 81. Tank lid; 810. Top cover; 8101. Transfer area; 811. First insulation layer; 812. Cold insulation layer; 82. Tank body; 820. Outer liner; 821. Insulated space; 822. Inner liner; 823. Connecting block; 824. Injection connector; 83. Locking assembly; 830. Locking hook; 8301. Snap-on part; 831. Button; 832. First elastic element; 833. Second elastic element; 84. Sealing cover; 840. Cover body ; 841, Operating section; 842, Second insulation layer; 85, Support frame; 850, Connecting rod; 851, Partition; 8510, Through groove; 8511, Liquid level indicator; 852, Compartment; 853, Guide rod; 8530, Guide surface; 8531, Push rod plate; 8532, Limiting protrusion; 854, Slide; 22, Vitrification assembly; 2233, Push rod contact plate; 91, Extraction clamp; 92, Tweezers; 93, Liquid nitrogen storage tank; 931, Storage cover; 932, Storage tank. Detailed Implementation

[0036] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0037] It should be noted that when a component is said to be "fixed to" another component, it can be directly on the other component or it can be fixed through another intermediate component. When a component is said to be "connected to" another component, it can be directly connected to the other component or it may be fixed through another intermediate component. When a component is said to be "set on" another component, it can be set directly on the other component or it may be set through another intermediate component. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0038] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0039] like Figures 1 to 8 As shown, the liquid nitrogen tank 80 for embryo cryopreservation transfer of the present invention is used for vitrification freezing of vitrification assembly 22, on which multiple embryos are mounted. The liquid nitrogen tank 80 for embryo cryopreservation transfer includes a tank lid 81, a tank body 82, a locking assembly 83, a sealing cap 84, and a support frame 85.

[0040] The can lid 81 includes an upper cover 810, a first insulation layer 811, and a cold insulation layer 812, with the first insulation layer 811 located between the upper cover 810 and the cold insulation layer 812. The upper surface of the upper cover 810 has a transfer area 8101 for temporarily placing a sealing cap 84. The can lid 81 has inlets, the number of which is the same as the number of sealing caps 84. The inlets penetrate the upper cover 810, the first insulation layer 811, and the cold insulation layer 812, and are used for inserting the vitrification assembly 22 into the can body 82. Each sealing cap 84 is detachably installed at one inlet. The position of each inlet corresponds to the position of the compartment 852.

[0041] The tank body 82 includes an outer liner 820, an inner liner 822, a connecting block 823, and an injection connector 824. The inner liner 822 is located inside the outer liner 820, forming an insulation space 821 between the inner liner 822 and the outer liner 820. The connecting block 823 is located at the top connection between the inner liner 822 and the outer liner 820. The injection connector 824 is installed on the outer liner 820 and the inner liner 822, connecting the insulation space 821 to external liquid nitrogen, allowing liquid nitrogen to be injected into the insulation space 821 through the injection connector 824.

[0042] The locking assembly 83 includes a locking hook 830, a button 831, a first elastic element 832, and a second elastic element 833. The locking hook 830 is rotatably mounted on the can lid 81, and the button 831 is slidably mounted on the can lid 81 and abuts against one end of the locking hook 830. The first elastic element 832 is sleeved on the button 831, with both ends abutting against the button 831 and the can lid 81 respectively. The first elastic element 832 provides a restoring force to the button 831. The locking hook 830 has a latching portion 8301 for latching with the can body 82. Specifically, the latching portion 8301 is J-shaped. The first elastic element 832 and the second elastic element 833 are springs. Both ends of the second elastic element 833 abut against the can lid 81 and the other end of the locking hook 830 respectively, and the second elastic element 833 provides a restoring force to the locking hook 830.

[0043] The sealing cap 84 includes a cap body 840, an operating part 841, and a second insulation layer 842. The operating part 841 extends from the upper surface of the cap body 840, and the second insulation layer 842 extends from the lower surface of the cap body 840. The operating part 841 facilitates the gripping of the sealing cap 84 by a robotic arm, and the second insulation layer 842 enables better insulation when the sealing cap 84 is installed on the can lid 81.

[0044] The support frame 85 includes a connecting rod 850, multiple partitions 851 fixed to the connecting rod 850, and multiple guide rods 853 fixed to the connecting rod 850. The connecting rod 850 is elongated and fixed to a connecting block 823. The multiple partitions 851 are parallel to each other, and a compartment 852 is formed between adjacent partitions 851. A compartment 852 is also formed between the partitions 851 and the tank body 82. The number of compartments 852 is the same as the number of sealing caps 84, and the positions of the sealing caps 84 correspond to the positions of the compartments 852, facilitating the placement of the vitrification assembly 22 in the compartments 852. The partitions 851 are provided with multiple through slots 8510 and two liquid level indicators 8511. The multiple through slots 8510 allow liquid nitrogen to flow freely inside the inner liner 822 and reduce the weight of the partitions 851, saving materials. The two liquid level indicators 8511 are used to indicate the amount of liquid nitrogen to be added during pre-cooling and vitrification freezing, respectively. Each compartment 852 contains two guide rods 853, each with a guide surface 8530. The guide surface 8530 is inclined, forming a slide 854 between the two guide rods 853. The length of the slide 854 is less than the length of the compartment 852. The guide surface 8530 extends to the end of the guide rod 853. Within the slide 854, the vitrified assembly 22 is guided by the slide 854, subjected to its own weight, the buoyancy of liquid nitrogen, and the separation effect of the partition 851, causing it to flip and eventually be placed horizontally in the storage compartment 852. A single compartment 852 can hold a maximum of 4 vitrified assemblies 22, and the four compartments 852 can hold a total of 16 vitrified assemblies 22 (placed horizontally). The total vitrification and freezing time for the 16 vitrified assemblies 22 is approximately 4 hours. After 5 hours of storage, the liquid nitrogen level in the embryo freezing transfer liquid nitrogen tank 80 will drop from its highest point to the critical height of the 16 vitrified assemblies 22 (placed horizontally). 16 vitrified embryos were cryopreserved in compartment 852, totaling 22 vitrified components.

[0045] Please continue reading. Figures 9 to 10 This is a second embodiment of the support frame 85. In the second embodiment, the structure of the support frame 85 is roughly the same as that of the first embodiment, except that the length of the slide 854 is equal to the length of the compartment 852, and the guide rod 853 is also provided with a limiting protrusion 8532, which is located at the end of the guide surface 8530. When the vitrified assembly 22 is placed in the slide 854, the push rod plate 8531 abuts against the vitrified assembly 22, the vitrified assembly 22 separates from the robot, and the push rod abutting plate 2233 is engaged with the guide surface 8530, so that the vitrified assembly 22 is vertically hung on the guide rod 853.

[0046] The vitrification components 22 are arranged sequentially along the guide rods 853, with a maximum of 8 vitrification components 22 per guide rod 853. The four compartments 852 can hold a total of 32 vitrification components 22 (vertically placed). Embryos in this type of cryopreservation are placed vertically within the vitrification components 22. The liquid nitrogen in the embryo cryopreservation transfer tank 80 will drop to the highest point (the critical point for embryos) after 1 hour. Therefore, this cryopreservation method requires adding liquid nitrogen to the embryo cryopreservation transfer tank 80 every 1 hour. Compartment 852 stores 32 vitrification components 22, totaling 128 embryos, requiring liquid nitrogen to be injected into the tank every 1 hour.

[0047] When assembling the liquid nitrogen transfer tank 80 for embryo cryopreservation, the tank cover 81 is rotatably mounted on the tank body 82, and the support frame 85 is fixed inside the tank body 82. A locking assembly 83 is installed on the tank cover 81 and locked to the tank body 82. A sealing cap 84 is installed on the tank cover 81.

[0048] Please continue reading. Figure 11 When using the embryo freezing transfer liquid nitrogen tank 80, liquid nitrogen is injected into the embryo freezing transfer liquid nitrogen tank 80, and the liquid nitrogen storage volume in the tank is monitored by a weighing sensor; a sealing cap 84 is moved from the inlet of the tank cap 81 to the transfer area 8101, so that the inlet corresponding to the compartment 852 to which the vitrification component 22 is to be placed is opened; the cryogenic robot clamps the vitrification component 22 into the compartment 852; the vitrification component 22 abuts against the support frame 85 to detach from the cryogenic robot and falls into the compartment 852; the vitrification component 22 with the embryo is vitrified in the embryo freezing transfer liquid nitrogen tank 80; after the vitrification is completed, the support frame 85 is transferred from the transfer liquid nitrogen tank to the lifting bucket in turn using tweezers 92, and then the lifting bucket is placed into the storage tank 932.

[0049] This invention also relates to a method for embryo cryopreservation transfer, implemented using the aforementioned liquid nitrogen tank 80 for embryo cryopreservation transfer, comprising the following steps:

[0050] Liquid nitrogen was injected into the liquid nitrogen tank 80 for embryo freezing and the amount of liquid nitrogen stored in the tank was monitored by a weighing sensor.

[0051] Move a sealing cap 84 from the inlet of the can lid 81 to the transfer area 8101, so that the inlet corresponding to the compartment 852 into which the vitrified component 22 is to be placed is opened;

[0052] The cryogenic robotic arm clamps the vitrification assembly 22 into compartment 852;

[0053] The vitrified component 22 abuts against the support frame 85 to detach from the cryogenic robotic arm and falls into the compartment 852;

[0054] The vitrification assembly 22 containing the embryo is vitrified and frozen in the liquid nitrogen tank 80 of the embryo cryopreservation transfer station;

[0055] After vitrification is completed, the vitrified components 22 are transferred from the intermediate liquid nitrogen tank to the bucket one by one using tweezers, and then the bucket is placed into the storage tank 932.

[0056] Frozen embryos require thawing before use. One vitrification assembly 22 holds four freezing dishes, with one embryo (blastocyst stage) in each dish. Embryo information (two-dimensional barcode) is stored at one end of the vitrification assembly 22. Before thawing, the embryo to be thawed must be removed from the vitrification assembly 22. The thawing and extraction process is as follows: ① The embryo cryopreservation liquid nitrogen tank 80 is pre-filled with liquid nitrogen (maximum level). ② The vitrification assembly 22 is removed from the extraction container and placed in the thawing station within the embryo cryopreservation liquid nitrogen tank 80. ③ The sealing cap is removed manually using forceps 92 (the sealing cap is magnetically connected to the carrier rod), the freezing dish is removed, and transferred to a constant temperature water bath (37℃).

[0057] The embryo freezing transfer liquid nitrogen tank 80 proposed in this invention can be used with an automated vitrification freezing system to achieve fully automated rapid embryo freezing. The embryo freezing transfer liquid nitrogen tank 80 has a storage capacity of 4L (maximum liquid level), and the storage time for an empty tank (without vitrification components 22) is 9 hours (until completely evaporated). Before use, the embryo freezing transfer liquid nitrogen tank 80 requires pre-cooling (pre-cooling the liquid level) for 10 minutes. The liquid nitrogen storage time in the embryo freezing transfer liquid nitrogen tank 80 is 6 hours, during which the liquid nitrogen completely evaporates from the maximum liquid level. The embryo freezing transfer liquid nitrogen tank 80 is equipped with two types of support racks 85. The first support rack 85 can freeze 16 vitrification components 22, totaling 64 embryos, without requiring additional liquid nitrogen. The second support rack 85 can freeze 32 vitrification components 22, totaling 128 embryos, requiring liquid nitrogen to be added every 1 hour. Liquid nitrogen is added to the embryo cryopreservation transfer liquid nitrogen tank 80 in two ways: ① manually pouring liquid nitrogen into the tank using a bucket; ② automatically adding liquid nitrogen via an external liquid nitrogen pump and injection connector 824. A weighing sensor monitors the liquid nitrogen level in the tank. When the liquid nitrogen level in the embryo cryopreservation transfer liquid nitrogen tank 80 reaches a warning value, the fully automatic vitrification system will prompt for additional liquid nitrogen. The embryo cryopreservation transfer liquid nitrogen tank 80 is equipped with dedicated extraction clips 91 and tweezers 92 for convenient operation. The dedicated extraction clips 91 allow for manual placement of the embryo cryopreservation transfer liquid nitrogen tank 80 into the fully automatic vitrification system / manual transfer of the transfer liquid nitrogen tank; the tweezers 92 assist in transferring the vitrification assembly 22 to the storage liquid nitrogen tank / embryo thawing and recovery. The vitrification assembly 22 has a cryo-disc thawing and extraction station. Using the dedicated tweezers 92, the cryo-disc inside the vitrification assembly 22 is transferred to a constant temperature water bath (37℃).

[0058] The above embodiments merely illustrate several implementation methods of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the invention patent. It should be noted that, for those skilled in the art, several modifications and improvements can be made without departing from the concept of the present invention. These are all equivalent modifications and improvements made to the above embodiments based on the essential technology of the present invention, and all of these fall within the protection scope of the present invention.

Claims

1. A liquid nitrogen tank for embryo cryopreservation, comprising a tank body and a tank lid, wherein the tank lid is installed on the tank body, and a cryopreservation space is formed between the tank body and the tank lid, characterized in that: The liquid nitrogen tank for embryo freezing transfer also includes a sealing cover and a support frame. There are multiple sealing covers, which are detachably installed on the tank cover. The support frame is installed in the freezing space. The support frame includes a connecting rod, multiple partitions fixed to the connecting rod, and multiple guide rods fixed to the connecting rod. A compartment is formed between adjacent partitions, and a compartment is formed between the partitions and the tank body. The number of compartments is the same as the number of sealing covers. The positions of the sealing covers correspond to the positions of the compartments. The guide rods are located in the compartments, and each guide rod has a pusher plate that abuts against the vitrification assembly, causing the vitrification assembly to fall into the compartment. Each compartment contains two guide rods, each guide rod has a guide surface, the guide surface is inclined, and a slide is formed between the two guide rods; The length of the slide is less than the length of the compartment, the guide surface extends to the end of the guide rod, and the vitrified assembly flips inside the slide and slides horizontally into the compartment; Alternatively, the length of the slide rail may be equal to the length of the compartment, and the guide rod may also be provided with a limiting protrusion located at the end of the guide surface, with the vitrification components arranged vertically in the slide rail.

2. The liquid nitrogen tank for embryo cryopreservation transfer according to claim 1, characterized in that: The can lid has multiple inlets and at least one transfer area. The number of inlets is the same as the number of sealing caps. Each sealing cap is installed at one of the inlets. The transfer area is used to temporarily place the sealing cap.

3. The liquid nitrogen tank for embryo cryopreservation transfer according to claim 2, characterized in that: The can lid includes an upper cover, a first insulation layer, and a cold insulation layer. The first insulation layer is located between the upper cover and the cold insulation layer, and the transfer area is located on the upper surface of the upper cover.

4. The liquid nitrogen tank for embryo cryopreservation transfer according to claim 1, characterized in that: The liquid nitrogen tank for embryo cryopreservation also includes a locking assembly, which includes a locking hook, a button, and a second elastic element. The locking hook is rotatably mounted on the tank lid, the button is slidably mounted on the tank lid and abuts against the locking hook, and the two ends of the second elastic element abut against the locking hook and the tank lid, respectively. The locking hook is snapped into the tank body.

5. The liquid nitrogen tank for embryo cryopreservation transfer according to claim 1, characterized in that: The tank includes an outer liner and an inner liner, the inner liner being located inside the outer liner, and the support frame being installed inside the inner liner.

6. The liquid nitrogen tank for embryo cryopreservation transfer according to claim 1, characterized in that: The sealing cover includes a cover body, an operating part, and a second heat insulation layer. The operating part extends from the upper surface of the cover body, and the second heat insulation layer extends from the lower surface of the cover body.

7. A method for embryo cryopreservation transfer, implemented using the liquid nitrogen tank for embryo cryopreservation transfer as described in any one of claims 1-6, characterized in that, Includes the following steps: Liquid nitrogen is injected into the liquid nitrogen tank for embryo freezing and the amount of liquid nitrogen stored in the tank is monitored by a weighing sensor. Move a sealing cap from the can lid inlet to the transfer area, so that the inlet corresponding to the compartment to be placed into the vitrification component is opened; The cryogenic robotic arm clamps the vitrified assembly into the compartment; The vitrified component comes into contact with the support frame to detach from the cryogenic robotic arm and falls into the compartment; The vitrification assembly containing the embryo is vitrified in the liquid nitrogen tank of the embryo cryopreservation transfer station; After vitrification is completed, the vitrified components are transferred one by one from the intermediate liquid nitrogen tank to the bucket using tweezers, and then the bucket is placed into the storage tank.

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