Zuogui pill serum containing active ingredients of Zuogui pill serum metabolic substance groups and preparation method and application of Zuogui pill serum

A technology of Zuogui Pills and serum, which is applied in the field of Zuogui Pills serum and its preparation, can solve the problems that Zuogui Pills serum has not been reported, and achieve the effects of improving embryonic development rate, increasing content, and promoting embryonic development

Inactive Publication Date: 2017-02-22
SHANXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Zuogui Pill serum containing active components of Zuogui Pill serum metabolite group has not been reported

Method used

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  • Zuogui pill serum containing active ingredients of Zuogui pill serum metabolic substance groups and preparation method and application of Zuogui pill serum
  • Zuogui pill serum containing active ingredients of Zuogui pill serum metabolic substance groups and preparation method and application of Zuogui pill serum
  • Zuogui pill serum containing active ingredients of Zuogui pill serum metabolic substance groups and preparation method and application of Zuogui pill serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1, Preparation and Component Analysis of Zuogui Pill Serum Sample

[0035] 1. Preparation of Zuogui Pill Serum Samples

[0036] Prepare Zuogui pill serum samples according to the following steps:

[0037] 1. Preparation of Zuoguiwan Water Extract

[0038]According to the mass ratio of rehmannia glutinosa: yam: cornus: medlar: dodder: antler gum: tortoise shell gum: Sichuan Achyranthes bidentata = 8:4:4:4:4:4:4:3, the medicinal materials were weighed. Except for antler glue and tortoise shell glue, mix all medicinal materials and add water to decoct three times at 50°C. Among them, the first decoction is to take 8 times the amount (w / v) of water and decoct for 1.5h, and the second decoction Take 8 times the amount (w / v) of water to decoct for 1.5 hours, and the third decoction is to take 8 times the amount of water (w / v) to decoct for 1.5 hours, and combine the three extractions to filter through eight layers of gauze. Take tortoise shell glue and deer antler ...

Embodiment 2

[0063] Embodiment 2, the influence of Zuogui pill serum of the present invention on mouse embryo development in vitro

[0064] 1. Preparation of Zuogui Pill Serum Samples

[0065] The serum samples of Zuogui Pills were prepared according to the same steps as in Example 1.

[0066] 2. Reagents and instruments used in the experiment

[0067] All chemicals were purchased from Sigma Corporation (St. Louis, MO, USA). PMSG and HCG were purchased from Ningbo Hormone Products Co., Ltd., hyaluronidase, operating solution, KSOM culture solution, DAPI staining solution, ultra-clean bench (Nu-301-330E British NUVNR company), stereo microscope (261 Japan Olympus company), fluorescence microscope (BX60 Japan Olympus company), CO 2 Incubator (3110 American Forma Company).

[0068] 3. Experimental content and methods

[0069] 1. Collection and in vitro culture of fertilized eggs

[0070] Inject PMSG 7.5IU / mouse intraperitoneally, inject HCG 7.5IU / mouse 48h later for superovulation, and ...

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PUM

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Abstract

The invention discloses Zuogui pill serum containing active ingredients of Zuogui pill serum metabolic substance groups and a preparation method and application of the Zuogui pill serum. The preparation method includes the steps of (1), mixing medicinal materials for preparing Zuogui pills according to a formulation dosage for preparing the Zuogui pills and performing water extraction so as to obtain water extract; (2), administrating the water extract in animals at a dosage of 10-20 g kg<-1> d<-1> for 7-10 days continuously, once to twice per day, collecting blood after administration, and collecting serum after blood centrifugation so as to obtain the Zuogui pill serum. The Zuogui pill serum is highly rich in glycoside active ingredients and is capable of remarkably increasing an inhibited embryo development rate under intervention of high glucose to promote embryo development.

Description

technical field [0001] The invention belongs to the technical field of medicinal chemistry of traditional Chinese medicine serum, and in particular relates to Zuogui pill serum containing effective components of Zuogui pill serum metabolite group and its preparation method and application. Background technique [0002] Taking the serum after oral administration of traditional Chinese medicine as a sample, according to the same research method of traditional medicinal chemistry, a variety of modern technologies are applied comprehensively to separate and identify the migration components from the serum, to study the correlation between the migration components in the serum and the traditional curative effect, and to elucidate the in vivo direct effect. The method of substance metabolism and in vivo dynamics is called serum medicinal chemistry of traditional Chinese medicine, which has important research significance in quality standardization research, elucidation of compound ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073A61K35/16A61P15/00
CPCC12N5/0604A61K35/16C12N2500/76C12N2500/84
Inventor 王颖莉许凯霞宋强冯前进
Owner SHANXI UNIV OF CHINESE MEDICINE
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