14results about "Cell membrane modification" patented technology

The purpose of the present invention is to provide a novel coating film formation composition for the formation of a coating film having a function of inhibiting adhesion of a biological substance, a coating film using said composition, and manufacturing methods for a substrate for a cell culture, a multilayer film, and a cell aggregate using the coating film.　The present invention provides a coating film formation composition including: a polymer that includes structural units represented by formula (1) and formula (2) (in the formulas, R1-R3, X1, X2, T1, n1, n2, and n3 are as set forth in the specification and the claims) and that does not include a polysiloxane skeleton; and a solvent.

The present invention provides an oligodeoxynucleotide of unmethylated cytosine-phosphate-guanine dinucleotide and B lymphocytes bound thereto, which can target migration to diseased lymphoid organs and inhibit B lymphocytes activated under pathological conditions, thereby having a targeted therapeutic effect against pathological damage caused by B lymphocyte activation.

The present invention provides methods and means for producing nitrogenase polypeptides in the mitochondria of plant cells. The present invention provides methods and means for producing nitrogenase polypeptides in the mitochondria of plant cells.

The present disclosure provides a surface modified immune cell comprising (i) a linker that is covalently bound to an immune cell surface and (ii) a non-antibody ligand covalently bound to said linker, wherein said ligand is selective to a receptor that is present in a target cell. The present disclosure also provides a method for producing and using the same.

The invention relates to a method for obtaining crystallised foetal bovine serum by adding agmatine that, at a specific concentration, acts to increase cell production and increase protein expression

The application belongs to the field of biomedical engineering and bionic robot technology, and particularly relates to a MOF modified myocardial cell material, a preparation method and application thereof, and a bionic driver. The MOF modified myocardial cell material provided by the application comprises myocardial cells and MOF material anchored on the cell membrane surface of the myocardial cells through coordination bonds, and the MOF material comprises UiO-66(Zr) and / or functionalized UiO-66(Zr), wherein the functionalized UiO-66(Zr) comprises aminated UiO-66(Zr) and / or nitrated UiO-66(Zr). The MOF modified myocardial cell material provided by the application realizes precise regulation of the calcium ion dynamics and contraction performance of myocardial cells, can significantly accelerate the contraction frequency of myocardial cells while improving the contraction force and mechanical stiffness of myocardial cells, and is crucial for constructing a high-performance and sustainable bionic driver.

The present disclosure provides compositions and methods of use of nucleic acids and complexes thereof targeted to nucleic acids. Genomic engineering can refer to the alteration of a genome by deletion, insertion, mutation, or replacement of a particular nucleic acid sequence. The alteration can be gene or position specific. Genomic engineering can utilize nucleases to cleave nucleic acids, thereby generating a site for alteration. Engineering of non-genomic nucleic acids is also contemplated.

To provide a recombinant protein for effective drug delivery, and to provide modified mitochondria produced using the same.SOLUTION: Provided are modified mitochondria in which a foreign protein is bound to the outer mitochondrial membrane.SELECTED DRAWING: None

Improved methods for large scale production of proteins and / or polypeptides in cell culture is provided. In accordance with the present invention, the method provides for culturing cells that have metabolically shifted. The use of such a method or system allows high levels of protein or polypeptide production and reduces accumulation of unwanted metabolic waste such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, or other commercial biologic compositions, such as antibodies.

The present invention addresses the problem of providing a culture medium for long-term culture of hepatocytes while maintaining the bile canaliculi thereof. The present invention relates to a medium for culturing hepatocytes while maintaining the bile canaliculi that contains, as an active ingredient, one or more selected from the group consisting of AMPK activators, Epac activators, apoptosis inhibitors, and antioxidants, and to a method for maintaining the bile canaliculi that includes culturing hepatocytes using the medium.

Provided are a perfusion culture method and the like that are able to improve the productivity in the production of recombinant protein. Specifically, a method for improving recombinant protein production in perfusion culture of an animal cell having a capacity to produce a recombinant protein, the method comprising adding cystine and / or ammonium iron citrate to a cell culture medium, and the like, are provided.

Described herein are compositions and techniques related to generation and therapeutic application of engineered extracellular vesicles. Engineered extracellular vesicles are vesicles (i.e., exosomes) comprising at least one engineered fusion polypeptide further comprising a vesicle targeting domain, scaffold linker domain, and signaling domain (i.e., a multi-effector signaling domain) wherein the signaling domain targets and interacts with a biological target, such as cell surface receptors. Said engineered fusion proteins can be organized in genetic vector constructs, expressed in mammalian cells, wherein the vesicle targeting domains anchor to extracellular vesicles such as exosomes, thereby presenting their joined multi-effector signaling domain which can relay a signal to target cells. Engineered extracellular vesicles adopt the hallmark biophysical and biochemical features of extracellular vesicles, allowing for rapid deployment and scale-up. Importantly, this strategy can allow for kinetically favorable signal generation and signal propagation to a target cell.

The present invention provides methods of making immune effector cells (e.g., T cells, NK cells) that can be engineered to express chimeric antigen receptors (CARs), as well as compositions and reaction mixtures comprising the cells.