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72 results about "Protein transfer" patented technology
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PROTEIN TRANSFER. After electrophoresis is complete, proteins must be transferred from the gel onto a suitable membrane for antibody staining and detection. Transfer is performed by passing a current across the gel to the membrane.
Auto-stimulating cells and methods for making and using the same
Methods for transferring one or more proteins to a cell are disclosed. The protein or proteins to be transferred are in the form of a fusion protein, and contain at least one domain encoding for a protein or peptide having trans signaling and / or adhesion function. The fusion protein is transferred to a cell by binding to a lipidated protein, which has been incorporated into the cell membrane. In an additional aspect of the invention, methods of making fusion proteins having cis signaling capabilities, as well as the ability to bind with receptors on the cell's own surface, are provided. Fusion proteins incorporating GPI or a homing element, and a costimulator or inhibitor domain can also be directly transferred to the cell surface. Methods for using cells which have undergone protein transfer according to the present methods are also disclosed. This includes use in a cancer vaccine, use for treatment of cancer or autoimmune disease, and use in determining costimulator threshold levels.
Owner:THE TRUSTEES OF THE UNIV OF PENNSYLVANIA
Analysis of microbes from microcolonies by maldi mass spectrometry
ActiveUS20130337502A1Low costHigh sensitivityParticle separator tubesMicrobiological testing/measurementDesorptionBiology
The invention relates to the cell disruption of microbes and the preparation of the microbe proteins for mass spectrometric analysis. The cells of microbes from microcolonies are disrupted by physical or chemical means directly on the nutrient medium. The released proteins are then transferred to sample supports by direct contact with their contact surfaces; electrophoresis can be used for assistance. Once the the proteins are firmly adsorbed on the contact surfaces, they can be washed with water in order to remove substances which interfere with the ionization process. For analysis by matrix-assisted laser desorption (MALDI), the proteins are prepared on the contact surfaces of the sample supports with matrix substances to form MALDI samples; the sample supports are then introduced into a MALDI mass spectrometer for the acquisition of the mass spectra. The microbes are identified by similarity comparisons between the mass spectra of the microbe proteins and similarly obtained reference spectra.
Owner:BRUKER DALTONIK GMBH & CO KG
Lipidic protein-transfer systems and cosmetic/dermatological use thereof
InactiveUS20070053852A1Improved lipid transferEmulsion stabilizationCosmetic preparationsHair cosmeticsLipid formationCarrier system
The present invention relates to compositions having bioactive transfer proteins in stable carrier systems. Such systems are above all suitable for influencing / changing lipid cell structures by means of improved lipid exchange, while at the same time having great stability. In this connection, not only transfer proteins and lipid components, but also protective colloids are used, by means of which an impairment of the transfer effect of the proteins and a loss of stability can be avoided. The compositions are suitable for external topical use for cleansing, care, dermatological and / or therapeutic treatment of the skin, hair, or mucous membranes.
Owner:MERZ PHARMA GMBH & CO KGAA
Stain-free protein quantification and normalization
Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.
Owner:BIO RAD LAB INC
Auto-stimulating cells and methods for making and using the same
InactiveUS7435585B2Permit deliveryPeptide/protein ingredientsAntibody mimetics/scaffoldsTrans signalingAutoimmune condition
Methods for transferring one or more proteins to a cell are disclosed. The protein or proteins to be transferred are in the form of a fusion protein, and contain at least one domain encoding for a protein or peptide having trans signaling and / or adhesion function. The fusion protein is transferred to a cell by binding to a lipidated protein, which has been incorporated into the cell membrane. In an additional aspect of the invention, methods of making fusion proteins having cis signaling capabilities, as well as the ability to bind with receptors on the cell's own surface, are provided. Fusion proteins incorporating GPI or a homing element, and a costimulator or inhibitor domain can also be directly transferred to the cell surface. Methods for using cells which have undergone protein transfer according to the present methods are also disclosed. This includes use in a cancer vaccine, use for treatment of cancer or autoimmune disease, and use in determining costimulator threshold levels.
Owner:THE TRUSTEES OF THE UNIV OF PENNSYLVANIA
Electrochemical method for detecting activity of protein acetyltransferase
ActiveCN109187708AGood choiceGood repeatabilityMaterial electrochemical variablesCholine acetyltransferaseAmino acid
The invention discloses an electrochemical method for detecting the activity of protein acetyltransferase. P2 templated silver nanoparticles and cucurbit [8] urea are added into a P1 modified gold electrode and a detection system of a p300 protein, the activity of the p300 protein is detected by electrochemical measurement, and the protein transferase activity is obtained; the amino acid sequenceof P1 is 11-mercaptoundecanoic acid (MUA)-GGGFRGKGGKGLGKGGAKA; and the amino acid sequence of P2 is FGGGASLWWSEKL. p300 is used as a model, and the silver nanoparticles are synthesized by a signal template P2, and assembled with a sensing template P1 through a cavity, so that the sensitivity of detection is ensured, high design flexibility is achieved, and meanwhile, the selectivity of HATs is enhanced. The development of the simple and practical electrochemical method capable of detecting HATs activity is expected to help early diagnosis of major diseases.
Owner:SUZHOU CHIEN SHIUNG INST OF TECH
Western blot analytical technique
ActiveCN102918387AWithdrawing sample devicesMaterial analysis by electric/magnetic meansProtein targetProtein transfer
The invention relates to a western blot analytical technique. In accordance with an embodiment of the present invention, there is provided a method of performing the western blot analytical technique, wherein the method comprises carrying out the following steps in the following order: a), pre-staining the proteins within a sample; b). separating the proteins using gel electrophoresis; c). analysing the separated proteins to determine the total protein load and / or at least one housekeeping protein load; d). transferring the proteins onto at least one membrane; e). probing the separated proteins to detect a target protein; and f). analysing the probed proteins to determine the target protein's molecular weight and / or load.
Owner:AGILENT TECH INC
Analysis of microbes from microcolonies by maldi mass spectrometry
ActiveUS8835176B2Solve the lack of resolutionAvoid mistakesComponent separationSamples introduction/extractionDesorptionBiology
The invention relates to the cell disruption of microbes and the preparation of the microbe proteins for mass spectrometric analysis. The cells of microbes from microcolonies are disrupted by physical or chemical means directly on the nutrient medium. The released proteins are then transferred to sample supports by direct contact with their contact surfaces; electrophoresis can be used for assistance. Once the proteins are firmly adsorbed on the contact surfaces, they can be washed with water in order to remove substances which interfere with the ionization process. For analysis by matrix-assisted laser desorption (MALDI), the proteins are prepared on the contact surfaces of the sample supports with matrix substances to form MALDI samples; the sample supports are then introduced into a MALDI mass spectrometer for the acquisition of mass spectra. The microbes are identified by similarity comparisons between the mass spectra of the microbe proteins and similarly obtained reference spectra.
Owner:BRUKER DALTONIK GMBH & CO KG
Device and method for transferring and analyzing proteins in gel on line
InactiveCN103163194AEfficient transferFast transferPreparing sample for investigationMaterial electrochemical variablesProtein transferProtein insertion
The invention discloses a device for transferring and analyzing proteins in gel on line. The device comprises a three-electrode power supply, a capillary, a first buffering liquid tank, a second buffering liquid tank, a detector and a metal needle tube. During transferring, the metal needle tube and the zero electrode of the three-electrode power supply are connected, are sleeved on the sample introduction end of the capillary and are placed on the upper surface of the gel; the outlet end of the capillary and the positive electrode of the three-electrode power supply are placed in the first buffering liquid tank; and the negative electrode of the three-electrode power supply is connected to the gel. During analyzing, the zero electrode of the three-electrode power supply and the sample introduction end of the capillary are placed in the second buffering liquid tank; and the outlet end of the capillary and the positive electrode of the three-electrode power supply are still placed in the first buffering liquid tank. Compared with other protein transferring methods, the device can be used for transferring and detecting simply, fast, efficiently and one line, avoid the influence of dyeing on the transferring of a protein strip in SDS-PAGE, has important theory and practical values on gel protein transferring and has very wide practical application prospect.
Owner:TIANJIN UNIV
Nanopore sensor for enzyme-mediated protein translocation
ActiveUS20160032236A1Improving “ threading ”Bioreactor/fermenter combinationsBiological substance pretreatmentsEscherichia coliProtein insertion
Described herein is a device and method for translocating a protein through a nanopore and monitoring electronic changes caused by different amino acids in the protein. The device comprises a nanopore in a membrane, an amplifier for providing a voltage between the cis side and trans side of the membrane, and an NTP driven unfoldase which processed the protein to be translocated. The exemplified unfoldase is the ClpX unfoldase from E. coli.
Owner:RGT UNIV OF CALIFORNIA
Safety evaluation method of transferring Bt gene insect-resistant paddy for predator paederus fuscipes
InactiveCN104322452ASimple and fast operationLow costBiological testingAnimal husbandryThree levelPotential toxicity
The invention relates to a safety evaluation method of transferring Bt gene insect-resistant paddy for predator paederus fuscipes. The method comprises three parts which are three-level nutrition test, protein transfer rule and Tier-1 toxicity determination test, wherein each part evaluates the influence on the paederus fuscipes by the transferring Bt gene insect-resistant paddy through prey mediation, the approach and degree of the paederus fuscipes exposed to Bt protein, and the potential toxicity of the Bt protein expressed by the transferring Bt gene insect-resistant paddy to the paederus fuscipes, and finally ecological results to non-target organism by the transferring Bt gene insect-resistant paddy are comprehensively evaluated according to data on the three aspects. The evaluation method is systematic and scientific, and has important significance on perfecting the research on the influence of transferring Bt gene crop on non-target organisms and confirming the ecological safety of transferring Bt gene crop planting.
Owner:HUAZHONG AGRI UNIV
Dry protein transfer
InactiveUS20160231272A1Leave gapMaterial analysis by electric/magnetic meansProtein transferElectrophoresis
Kits, systems, and methods are provided for transferring biological macromolecules from an electrophoresis slab gel to a blotting membrane using conductive polymer electrodes.
Owner:BIO RAD LAB INC
Gradient gel and preparation method thereof
InactiveCN103575792AHigh transfer rateImprove transfer qualityMaterial analysis by electric/magnetic meansElectrophoresisProtein transfer
The invention discloses a gradient gel and a preparation method thereof. The preparation method is characterized by comprising the following steps: respectively blending water, acrylamide separation gel stock solution, separation gel buffer solution, SDS (sodium dodecyl sulfate) and ammonium persulfate into two or more separation gels with concentration of 8-15% according to a certain proportion, quickly pouring the blended two or more separation gels in different concentrations into a glass plate crack in vertical slab electrophoresis from high to low concentration in order, adding water, adding spacer gel after the separation gel is solidified, and waiting the solidification of the spacer gel. According to the method, the electrophoresis speed is increased, the penetrability of protein molecules is enhanced, and the protein transfer rate and the protein transfer quality are improved.
Owner:GUANGZHOU WONDFO BIOTECH
Western blot analytical technique
InactiveUS20130115714A1Accurate mappingQuantitative precisionMaterial analysis by electric/magnetic meansProtein targetProtein transfer
In accordance with an embodiment of the present invention, there is provided a method of performing the western blot analytical technique, wherein the method comprises carrying out the following steps in the following order: a), pre-staining the proteins within a sample; b). separating the proteins using gel electrophoresis; c). analysing the separated proteins to determine the total protein load and / or at least one housekeeping protein load; d). transferring the proteins onto at least one membrane; e). probing the separated proteins to detect a target protein; and f). analysing the probed proteins to determine the target protein's molecular weight and / or load.
Owner:AGILENT TECH INC
Preparation method of high-molecular-weight silver carp skin collagen peptide
The invention relates to a preparation method of high-molecular-weight silver carp skin collagen peptide. The method includes the following steps of firstly, cleaning fresh silver carp skin to remove impurities, conducting degreasing through an alkali solution, removing impurity protein through an acid solution, and conducting rinsing to be nearly neutral; secondly, adding water to preprocessed silver carp skin, adjusting pH to be 3.5 to 4.5 and the temperature to be 40 DEG C to 45 DEG C, adding pepsase for an enzymatic hydrolysis reaction for the reaction time of 10 h to18 h, and keeping the pH and the temperature constant in the reaction process; thirdly, conducting ultra-low-temperature vacuum concentration on enzymatic hydrolysate of pepsase; fourthly, controlling the reaction conditions for a protein transfer reaction to obtain a large-molecule collagen peptide solution, and then conducting processing on the collagen peptide solution. By means of the method, protein synthesis is conducted on the basis of protein hydrolysis through the protein transfer reaction under the control conditions, large-molecule collagen peptide is obtained, molecular weight distribution is concentrated, raw materials and auxiliary materials are high in utilization rate, the process is simple, and processing cost is low.
Owner:武汉梁子湖水产品加工有限公司
Stain-free protein quantification and normalization
Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.
Owner:BIO RAD LAB INC
Culture method for improving stress resistance of snakehead fry
InactiveCN106538425AImprove commodityImprove conversion abilityClimate change adaptationAnimal feeding stuffJuvenile fishNutrient
The invention belongs to the technical field of aquaculture, and particularly relates to a culture method for improving the stress resistance of snakehead fry. According to the culture method for improving the stress resistance of the snakehead fry, corresponding conditions and feed are controlled in corresponding stages of the fry. Compared with the prior art, the culture method for improving the stress resistance of the snakehead fry has the following advantages that corresponding culture environments are controlled for culture ponds of the snakehead fry according to growing characteristics of the snakehead fry; the snakehead fry can absorb nutrients quickly through cooperation of the culture environments and the high-protein feed, the protein transfer ability is improved, immunity is reinforced, and the growing property is improved; the snakehead fry can grow to 8-9 cm in the 35-day age, and 3-7 days are advanced compared with the prior art; after culture is completed, the snakehead fry are transferred into culture ponds for adult fish culture, quick adaption can be achieved, and the survival rate reaches 97% and over; after culture is completed, fish meat is rich in nutrient, good in taste, high in commodification and remarkable in economic benefits.
Owner:王玉宽
Temperature controllable switching type nano-filtration membrane and preparation method thereof
ActiveCN107281943ASmall apertureEfficient Control TransmissionReverse osmosisFiltration membraneProtein transfer
The invention relates to a temperature controllable switching type nano-filtration membrane. The temperature controllable switching type nano-filtration membrane comprises a filtration membrane body and a carbon nanotube array which penetrates through the filtration membrane body, wherein the carbon nanotube array comprises a plurality of carbon nanotubes which are arrayed in parallel; the carbon nanotubes are grafted with temperature-sensitive macromolecular polymer material layers; the temperature-sensitive macromolecular polymer material layers are at a contracted or extending state along temperature changes, so that a pore diameter size of a channel of the temperature controllable switching type nano-filtration membrane is changed along the temperature changes; a preparation method comprises the following steps: taking the carbon nanotubes and poly(N-isopropyl acrylamide) as raw materials and preparing the nano-filtration membrane with temperature controllable ions and protein transferring by adopting a chemical vapor deposition method and a surface-initiated atom transfer radical polymerization method; the nano-filtration membrane has good hole permeability and sensitive environment response and can realize controllable transferring and filtering of inorganic ions and proteins under different temperature.
Owner:QINGDAO UNIV
Gel electrophoresis and transfer combination using conductive polymers and method of use
ActiveCN108473531ASeparate visualizationSimple methodPeptide preparation methodsBiological testingElectricityProtein transfer
A precast gel and membrane combination unit for use in gel electrophoresis and membrane transfer for protein analysis, and method of use. Transparent electrically conductive polymer plate(s) house anelectrophoresis gel and immunoblotting membrane. The gel and membrane are positioned between two plates of conductive polymers or plates having an electrically conductive layer or film. During the electrophoresis step, electric current moves proteins through the gel allowing for protein separation. Then, without removing or reorienting the gel or apparatus, the electrical contacts are switched toallow the flow of electricity to run perpendicularly through the gel via the conductive poylmers, which will allow the proteins to transfer to the membrane housed in the same precast gel and membranecombination unit. The apparatus allows the user to visualize the steps of protein separation and protein transfer without transferring the gel between electrophoresis and transfer steps.
Owner:WOODHAM BIOTECH HLDG LLC
Efficient protein-to-membrane transferring buffer solution and preparation method thereof
InactiveCN105866220AReduce heatImprove buffering effectMaterial analysis by electric/magnetic meansElectrophoresisProtein molecules
The invention discloses a high-efficiency protein transfer membrane buffer, which comprises the following components: the concentration of Tris is 10mM-100mM; the concentration of taurine is 10mM-200mM. Compared with the traditional membrane transfer buffer commonly used at present, the high-efficiency membrane transfer buffer of the present invention uses a taurine component with a stronger buffer capacity, which improves the buffer capacity of the buffer during electrophoresis, and is suitable for proteins with a wide range of molecular weights. Molecules have high membrane transfer efficiency, which can shorten the time for protein transfer to the membrane. At the same time, the heat generated by the buffer during electrophoresis is low, and there is no need to use ice packs to cool down, which simplifies the operation during membrane transfer. The concentration of methanol is lower, and a better transfer effect can be achieved at a concentration of 10%.
Owner:SUZHOU NEW CELL & MOLECULAR BIOTECH CO LTD
High-throughput crystallographic screening device and method for crystalizing membrane proteins using a sub physiological resting membrane potential across a lipid matrix of variable composition
ActiveUS11440001B1Promote recoveryPolycrystalline material growthPeptide librariesLipidomeProtein transfer
The invention is a high-throughput voltage screening crystallographic device and methodology that uses multiple micro wells and electric circuits capable of assaying different crystallization condition for the same or different proteins of interest at the same of different voltages under a humidity and temperature controlled environment. The protein is solubilized in a lipid matrix similar to the lipid composition of the protein in the native environment to ensure stability of the protein during crystallization. The invention provides a system and method where the protein is transferred to a lipid matrix that holds a resting membrane potential, which reduces the degree of conformational freedom of the protein. The invention overcomes the majority of the difficulties associated with vapor diffusion techniques and essentially reconstitutes the protein in its native lipid environment under “cuasi” physiological conditions.
Owner:UNIVERSITY OF PUERTO RICO
Support For Protein Transfer, Protein Transfer Agent Using the Support, Protein Transfer Method, Cell Having Protein Transferred Thereinto and Method of Producing the Same
InactiveUS20080182786A1Raise security concernsEasy to introducePowder deliveryPeptide/protein ingredientsProtein targetClay minerals
A protein introduction method with which protein can be introduced into cells with excellent safety is provided. A target protein is supported on a carrier for protein introduction that is made from a clay mineral, and by adding this to cells it is possible to introduce the target protein into the cells. The clay mineral is preferably a layered clay mineral, and as the clay mineral it is possible to use montmorillonite, vermiculite, and illite, for example.
Owner:MEIJI CO LTD
Dry protein transfer
Kits, systems, and methods are provided for transferring biological macromolecules from an electrophoresis slab gel to a blotting membrane using conductive polymer electrodes.
Owner:BIO RAD LAB INC
Naphthyl urea-piperazine compound as well as preparation method and application thereof
The invention discloses naphthyl urea-piperazine compounds and a preparation method and application thereof.The naphthyl urea-piperazine compounds can remarkably inhibit activation of cell signal transduction JAKs / STATs signals under the low dosage, and the experimental results of a cell proliferation experiment, an immunoblotting experiment, a cell cycle experiment, a protein transfer membrane transport experiment, a cell invasion and migration experiment and the like show that the naphthyl urea-piperazine compounds have the advantages that the naphthyl urea-piperazine compounds can be used for remarkably inhibiting activation of cell signal transduction JAKs / STATs signals; the compound can specifically inhibit JAK2 signal activation and expression of downstream STAT3, CyclinD1, CyclinB1, MMP9 and other target genes, induce blocking of a cell cycle G1 phase and cell apoptosis, can significantly inhibit proliferation of breast cancer, liver cancer, lung cancer, colon cancer, leukemia, lymphoma, multiple myeloma, retinoblastoma and other tumor cell strains, and can be used for preparing drugs for treating breast cancer, liver cancer, lung cancer, colon cancer, leukemia, lymphoma, multiple myeloma, retinoblastoma and the like. Therefore, the compound has a prospect of being developed into a targeted anti-cancer drug of a related target spot of a JAKs / STATs cell regulation signal transduction pathway.
Owner:HENAN RADIOMEDICAL SCI & TECH CO LTD
Method for intracellular delivery of protein
InactiveCN112080458AGood biocompatibilityAvoid captureCulture processCell culture active agentsProtein solutionTransfer cell
The invention discloses a method for intracellular delivery of protein. The method comprises the following steps of (1) preparing a protein solution and a halogenatedboron cluster solution with certain concentrations; (2) slowly adding the halogenatedboron cluster solution into the protein solution, quickly and uniformly mixing the two solutions, and standing for a period of time; and (3) adding amixed solution of halogenatedboron clusters and protein into a standard complete culture medium, and co-culturing the mixed solution with to-be-transferred cells for a period of time. The halogenatedboron clusters can be used to deliver proteins across biofilms without modification by chemical bonds. The method for protein transferring can prevent capture by endosomes, is simple and easy to implement, low in cost and relatively high in transfer efficiency, and the halogenatedboron clusters are a protein transfer carrier with a wide prospect.
Owner:CHINA UNIV OF PETROLEUM (EAST CHINA)
Detection method of collagen terminal peptide
InactiveCN108872585AExpand the detection rangeEffective Telopeptide QualitativeBiological material analysisBiological testingElectrophoresisProtein transfer
The invention discloses a detection method of a collagen terminal peptide. The detection method comprises the following steps: (1) performing treatment and electrophoresis on a sample; (2) protein transfer printing; (3) closing and incubating: oscillating a transfer printing film obtained in step (2) into a closing solution; adding a collagen terminal peptide antibody to performing the primary antibody incubation for the transfer printing film, and then adding a secondary antibody to perform the incubation; and (4) performing the color developing reaction: adding the transfer printing film after the secondary antibody incubation of step (3) into a color developing solution to develop until a stripe appears, then adding distilled water to terminate the color developing reaction, and judgingthe type of the collagen terminal peptide in a to-be-detected sample according to the stripe. The invention provides the method which can effectively qualitatively and half-quantitatively detect thecontent of terminal peptide in exogenous collagen, and provides method evidence for the immunogenicity of an exogenous collagen-based biological product.
Owner:GUANGZHOU TRAUER BIOTECH
Gel Electrophoresis and Transfer Combination using Conductive Polymers and Method of Use
ActiveUS20170356876A1Fast and reliable and easySimple methodMaterial analysis by electric/magnetic meansElectricityPower flow
A precast gel and membrane combination unit for use in gel electrophoresis and membrane transfer for protein analysis, and method of use. Transparent electrically conductive polymer plate(s) house an electrophoresis gel and immunoblotting membrane. The gel and membrane are positioned between two plates of conductive polymers or plates having an electrically conductive layer or film. During the electrophoresis step, electric current moves proteins through the gel allowing for protein separation. Then, without removing or reorienting the gel or apparatus, the electrical contacts are switched to allow the flow of electricity to run perpendicularly through the gel via the conductive polymers, which will allow the proteins to transfer to the membrane housed in the same precast gel and membrane combination unit. The apparatus allows the user to visualize the steps of protein separation and protein transfer without transferring the gel between electrophoresis and transfer steps.
Owner:WOODHAM BIOTECH HLDG LLC
Application of polyvinylidene fluoride microporous filter membrane and western blotting method
ActiveCN119139940AGood transferabilityEasy to detectMembranesUltrafiltrationProtein transferMicrofiltration membrane
The invention belongs to the technical field of protein transfer printing, and provides application of a polyvinylidene fluoride microporous filter membrane and a protein immunoblotting method.The surface of the polyvinylidene fluoride microporous filter membrane is provided with spherocrystal and rod-like short fiber crystals and is free of a compact skin layer. According to the application, the polyvinylidene fluoride microfiltration membrane serves as a solid-phase carrier in the western blotting process to conduct high-sensitivity detection on a protein sample with the microgram-level content, and a clear protein band is obtained; the polyvinylidene fluoride microfiltration membrane is prepared by the following method: at a first temperature, dissolving polyvinylidene fluoride in a solvent, adding an additive, mixing to obtain a feed liquid, and cooling the feed liquid to a second temperature to obtain a membrane casting liquid; preparing the membrane casting solution into a liquid membrane; and carrying out steam induced phase separation on the liquid membrane, and then immersing the liquid membrane into a coagulating bath for coagulating and forming to obtain the polyvinylidene fluoride microporous filter membrane. The polyvinylidene fluoride microfiltration membrane prepared by the invention has a good protein transfer printing effect in a protein immunoblotting process, and the detection effect is particularly obviously improved.
Owner:MEMBRANE SOLUTIONS (NANTONG) CO LTD
Device for sequential protein transfer from a gel
Owner:GOVERNMENT OF THE UNITED STATES OF AMERICA DEPT OF THE HEALTH & HUMAN SERVICES THE
Device for sequential protein transfer from a gel
Owner:GOVERNMENT OF THE UNITED STATES OF AMERICA DEPT OF THE HEALTH & HUMAN SERVICES THE