Preparation method of high-molecular-weight silver carp skin collagen peptide

A high-molecular-weight, skin-collagen technology, applied in the field of protein peptide preparation in biotechnology, can solve problems such as high cost, non-centralized distribution, and complicated process, and achieve the effect of low cost and high utilization rate of raw materials

Active Publication Date: 2016-03-23
武汉梁子湖水产品加工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The molecular weights of the collagen peptides prepared by the above method are far apart, and the distribution is not concentrated. Finally, the collage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for preparing high molecular weight silver carp skin collagen peptide, the steps are as follows:

[0030] (1) Weigh 1000Kg of fresh silver carp skin, rinse to remove foreign matter, soak in 0.2% sodium carbonate solution 4 times the weight of the fish skin for 8 hours, rinse until nearly neutral, then soak in 0.1% hydrochloric acid solution 5 times the weight of the fish skin 3h, rinse to near neutral;

[0031] (2) Put the washed fish skin into the reaction tank, add 2000Kg of pure water, start stirring, adjust the pH of the mixture to 4.5 with dilute hydrochloric acid, heat up to about 42°C by passing steam to the jacket of the reaction tank, and wait until the temperature and pH of the mixture are constant Add 2Kg of pepsin, maintain the initial pH and temperature and stir for 12 hours;

[0032] (3) Concentrate the enzymolysis solution with ultra-low temperature vacuum concentration equipment, the concentration temperature is 40°C, and transfer it to the rea...

Embodiment 2

[0037] A method for preparing high molecular weight silver carp skin collagen peptide, the steps are as follows:

[0038] (1) Weigh 2000Kg of fresh silver carp skin, wash to remove foreign matter, soak in 0.3% sodium hydroxide solution 6 times the weight of the fish skin for 6 hours, rinse until nearly neutral, and then use 0.2% citric acid 5 times the weight of the fish skin Soak in the solution for 6 hours, rinse until nearly neutral;

[0039] (2) Put the washed fish skin into the reaction tank, add 3000Kg of pure water, start stirring, adjust the pH of the mixture to 4.4 with citric acid, heat up to about 45°C by passing steam to the jacket of the reaction tank, and wait until the temperature and pH of the mixture are constant Add 3Kg of pepsin, maintain the initial pH and temperature and stir for 15 hours;

[0040] (3) Concentrate the enzymolysis solution with ultra-low temperature vacuum concentration equipment, the concentration temperature is 45°C, and transfer it to t...

Embodiment 3

[0045] A method for preparing high molecular weight silver carp skin collagen peptide, the steps are as follows:

[0046] (1) Weigh 2500Kg of fresh silver carp skin, rinse to remove foreign matter, soak in 0.4% calcium hydroxide solution 5 times the weight of the fish skin for 10 hours, rinse until nearly neutral, and then use 0.1% nitric acid solution 4 times the weight of the fish skin Soak for 4 hours, rinse until nearly neutral;

[0047] (2) Put the washed fish skin into the reaction tank, add 4000Kg of pure water, start stirring, adjust the pH of the mixture to 3.5 with dilute hydrochloric acid, heat up to about 41°C by passing steam to the jacket of the reaction tank, and wait until the temperature and pH of the mixture are constant Add 2.5Kg of pepsin, maintain the initial pH and temperature and stir for 18 hours;

[0048] (3) Concentrate the enzymolysis solution with ultra-low temperature vacuum concentration equipment, the concentration temperature is 44°C, and trans...

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PUM

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Abstract

The invention relates to a preparation method of high-molecular-weight silver carp skin collagen peptide. The method includes the following steps of firstly, cleaning fresh silver carp skin to remove impurities, conducting degreasing through an alkali solution, removing impurity protein through an acid solution, and conducting rinsing to be nearly neutral; secondly, adding water to preprocessed silver carp skin, adjusting pH to be 3.5 to 4.5 and the temperature to be 40 DEG C to 45 DEG C, adding pepsase for an enzymatic hydrolysis reaction for the reaction time of 10 h to18 h, and keeping the pH and the temperature constant in the reaction process; thirdly, conducting ultra-low-temperature vacuum concentration on enzymatic hydrolysate of pepsase; fourthly, controlling the reaction conditions for a protein transfer reaction to obtain a large-molecule collagen peptide solution, and then conducting processing on the collagen peptide solution. By means of the method, protein synthesis is conducted on the basis of protein hydrolysis through the protein transfer reaction under the control conditions, large-molecule collagen peptide is obtained, molecular weight distribution is concentrated, raw materials and auxiliary materials are high in utilization rate, the process is simple, and processing cost is low.

Description

Technical field [0001] The present invention involves the field of protein peptide preparation in biotechnology, and specifically involves a method of preparation of polymer metamorphin -white fisheyl glue protein peptide with a transpressive protein reaction. Background technique [0002] Collagen is an extremely important structural protein in connective tissue. It is the protein with the most content and the most widely distributed in the animal, accounting for about 25%to 30%of the total protein in the body.The absorption and utilization rate is low, and the biotechnology is degraded to a collagen peptide peptide from the molecular weight to the range of 200 ~ 10000DA, so that the human body can effectively absorb.Study proves that the average molecular weight of protein hydrolysis is one of the most important factors that determine the nature of its biology. The physical and chemical characteristics of the protein peptide component are related to its molecular weight. [000...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 何秋生陈春松任涛李向阳张凤娇黎彩汪兰吴文锦
Owner 武汉梁子湖水产品加工有限公司
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