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Safety evaluation method of transferring Bt gene insect-resistant paddy for predator paederus fuscipes

A technology for safety evaluation and lepidopteran, which is applied in the field of ecological risk evaluation of Bt gene crops, can solve the problem of difficulty in finding high resistance to lepidopteran insect resistance, and achieves the effect of simple operation and low cost.

Inactive Publication Date: 2015-02-04
HUAZHONG AGRI UNIV
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Problems solved by technology

However, in rice and wild rice, it is difficult to find a source of resistance to lepidopteran insects

Method used

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  • Safety evaluation method of transferring Bt gene insect-resistant paddy for predator paederus fuscipes
  • Safety evaluation method of transferring Bt gene insect-resistant paddy for predator paederus fuscipes
  • Safety evaluation method of transferring Bt gene insect-resistant paddy for predator paederus fuscipes

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Embodiment Construction

[0030] The Bt-gene insect-resistant transgenic rice of the present invention selects cry1C gene transgenic rice T1C-19, which has high resistance to lepidopteran pests, and the non-transgenic parent selects Guangminghui 63 rice, and the rice seeds are all inherited from crops of Huazhong Agricultural University. Provided by the State Key Laboratory of Improvement. The rice seeds were grown to a height of 15 ± 5 cm in a 25 cm long × 20 cm wide × 3 cm high cultivation tray with Yoshida nutrient solution for the evaluation test. The conditions for cultivating rice seedlings are a temperature of 26±2° C. and a relative humidity of 80%.

[0031] The tested brown planthoppers of the present invention were collected from paddy fields in Wuhan, Hubei, and established populations on rice seedlings of T1C-19 and Minghui 63 respectively indoors, and reared for more than 10 generations. The green-winged ant-shaped Pteroptera was collected from rice fields in Xiaogan, Hubei, and the breed...

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Abstract

The invention relates to a safety evaluation method of transferring Bt gene insect-resistant paddy for predator paederus fuscipes. The method comprises three parts which are three-level nutrition test, protein transfer rule and Tier-1 toxicity determination test, wherein each part evaluates the influence on the paederus fuscipes by the transferring Bt gene insect-resistant paddy through prey mediation, the approach and degree of the paederus fuscipes exposed to Bt protein, and the potential toxicity of the Bt protein expressed by the transferring Bt gene insect-resistant paddy to the paederus fuscipes, and finally ecological results to non-target organism by the transferring Bt gene insect-resistant paddy are comprehensively evaluated according to data on the three aspects. The evaluation method is systematic and scientific, and has important significance on perfecting the research on the influence of transferring Bt gene crop on non-target organisms and confirming the ecological safety of transferring Bt gene crop planting.

Description

technical field [0001] The invention belongs to the technical field of ecological risk assessment of transgenic Bt crops, and in particular relates to a method for evaluating the safety of transgenic Bt gene insect-resistant rice against the predatory natural enemy Pteropterus cerevisiae. Background technique [0002] Rice is one of the most important food crops in the world, but it is also one of the most infested food crops, and the annual yield loss caused by insect infestation is about 10% (Chen Hao et al., 2009). Among them, Lepidoptera pests are one of the most important pest groups, especially rice leaf rollers, rice stem borers, and rice stem borers, which seriously threaten rice production. At present, the control of lepidopteran pests mainly depends on chemical insecticides, which not only increase the production cost, but also cause serious environmental pollution. High resistance, which in turn leads to more and more use of pesticides, thus leading to the 3R pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033G01N33/68
CPCA01K67/033G01N33/5014
Inventor 华红霞孟嘉蓉韩宇吴刚
Owner HUAZHONG AGRI UNIV
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