Kit and method for detecting CYP3A5 gene polymorphism

A gene polymorphism and polymorphism technology, applied in the field of fluorescence quantitative PCR, can solve the problems of low polymorphism sensitivity and specificity, low sensitivity and specificity, time-consuming and labor-intensive, and avoid false positives. Or false negative, good specificity, high accuracy effect
CN103436631AActive Publication Date: 2013-12-11刘辉
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
刘辉
Publication Date
2013-12-11

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Abstract

The invention relates to a kit and a method for detecting CYP3A5 gene polymorphism, belonging to the field of fluorescent quantitative PCR (Polymerase Chain Reaction). The kit comprises primers for detection and fluorescent probes for detection, wherein the primers for detection and the fluorescent probes for detection comprise at least one group of specific upstream and downstream primers for detecting CYP3A5*3 polymorphism of the CYP3A5 gene as well as a Taqman fluorescent probe, specific upstream and downstream primers for detecting CYP2C19*4 polymorphism of the CYP3A5 gene as well as a Taqman fluorescent probe, specific upstream and downstream primers for detecting CYP2C19*6 polymorphism of the CYP3A5 gene as well as a Taqman fluorescent probe, and specific upstream and downstream primers for detecting CYP3A5*7 polymorphism of the CYP3A5 gene as well as a Taqman fluorescent probe. The detection sensitivity and specificity of the kit are remarkably improved, the detection time is short, and prediction of drug dose and curative effect is facilitated.
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Description

technical field

[0001] The invention relates to the field of fluorescence quantitative PCR, in particular to a kit and method for detecting CYP3A5 gene polymorphism. Background technique

[0002] The CYP3A5 gene is located on human chromosome 7, with a total length of 31.8kb and 13 exons, encoding 502 amino acids. It is the main extrahepatic expression form of CYP3A subfamily. It shows higher expression levels than CYP3A4 gene in lung, kidney, breast, prostate and polymorphonuclear leukocytes, but has a similar coding amino acid sequence to CYP3A4 gene. substrates and the same regulatory pathways. CYP3A5 wild-type is defined as CYP3A5*1. Only individuals who carry at least one CYP3A5*1 allele can express CYP3A5 protein normally. For details of CYP3A5 gene polymorphism, please refer to the CYP allele database (http: / / www.cypalleles.ki .se). The single nucleotide polymorphisms (single nucleotide polymorphisms, SNPs) of CYP3A5 were first discovered in 1996 by Jounaidi et al....

Claims

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