The invention belongs to the technical field of genetic engineering, relates to a primer combination, a kit and a method for performing PCR-SSP (polymerase chain reaction-sequence specific primer) typing detection on a KIR (killer cell immunoglobulin-like receptor) gene. The primer combination consists of detection primers shown in SEQ ID NO. 1-65, wherein detection primers 2DL1 and 2DL2 form a group, 2DS3 and 2DP1 form a group, DRB1, 3DS1 and 3DL1 form a group, 3DP1, 2DL5 and 2DS1 form a group, 2DL3 and 2DL4 form a group, 2DL3, 2DS3 and 3DS1 form a group, 2DS4, 3DL1 and 2DL1 form a group, 2DS4 and 2DS5 form a group, 3DL3, 2DS5 and 3DL2 form a group, 2DP1 and 2DS2 form a group, 2DS2 forms a group, 2DL4, 3DL2 and 3DL3 form a group, and all the detection primers form 12 primer groups. Through the primer combination, the kit and the method, the accuracy and the reliability are high, the operation is simple and stable, and a result is easily judged.