Detection agent for ABO blood type gene and shaping method
A technology for ABO blood type and gene detection, which is applied in the direction of biochemical equipment and methods, microbial measurement/testing, etc., and can solve problems such as incorrect typing
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[0125] Example 1: Detection and identification of common genes and A2 subtype genes in the ABO system
[0126] A total of 13 pairs of specific primers were designed for the invented DNA sequence, and were commissioned to be synthesized by Shanghai Shenggong Company (see Table 1, 2). Each ABO blood type gene detection reagent contains 4 primers, of which 2 are sequence-specific primer subtypes Genes and common genes, the other 2 are internal control primers, the internal control primers are human growth hormone (HGH) gene-specific primers, HGH-A chain sequence: 5'-tgc ctt ccc aac cat tcc cctta-3', HGH- B-chain sequence: 5'-cca ctc acg gat ttc tgt tgt gtt tc-3', the length of the amplified product is 434bp.
[0127] This kit can detect the 4 most common alleles of A101, A102, B101, and O01 in the Chinese population and all the 6 A2 alleles of A201, A202, A203, A204, A205, and A206.
[0128] Table 1 Common ABO gene-specific primer sequences and detected alleles
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