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Detection agent for ABO blood type gene and shaping method

A technology for ABO blood type and gene detection, which is applied in the direction of biochemical equipment and methods, microbial measurement/testing, etc., and can solve problems such as incorrect typing

Inactive Publication Date: 2009-04-08
SHENZHEN BLOOD CENT
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AI Technical Summary

Problems solved by technology

[0012] If there are no specific primers for the Chinese A2 subtype gene in genetic testing, it will cause incorrect typing and may be mistaken for A1 type

Method used

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  • Detection agent for ABO blood type gene and shaping method
  • Detection agent for ABO blood type gene and shaping method
  • Detection agent for ABO blood type gene and shaping method

Examples

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Embodiment 1

[0125] Example 1: Detection and identification of common genes and A2 subtype genes in the ABO system

[0126] A total of 13 pairs of specific primers were designed for the invented DNA sequence, and were commissioned to be synthesized by Shanghai Shenggong Company (see Table 1, 2). Each ABO blood type gene detection reagent contains 4 primers, of which 2 are sequence-specific primer subtypes Genes and common genes, the other 2 are internal control primers, the internal control primers are human growth hormone (HGH) gene-specific primers, HGH-A chain sequence: 5'-tgc ctt ccc aac cat tcc cctta-3', HGH- B-chain sequence: 5'-cca ctc acg gat ttc tgt tgt gtt tc-3', the length of the amplified product is 434bp.

[0127] This kit can detect the 4 most common alleles of A101, A102, B101, and O01 in the Chinese population and all the 6 A2 alleles of A201, A202, A203, A204, A205, and A206.

[0128] Table 1 Common ABO gene-specific primer sequences and detected alleles

[0129]

[0...

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Abstract

The invention discloses a detection reagent and a typing method for the simultaneous genotyping of antigens of ABO blood group and specific A2, comprising four primers, two of which are sequential specific primers and the other two of which are inner comparison primers. The method can carry out simultaneous amplification to the blood group genes of the antigens of the ABO blood group and the specific A2 subtypes, which are very common among the Chinese population. The method can detect four alleles, A101, A102, B101 and O01, and six A2 alleles, A201, A202, A202, A204, A205 and A206, which are very common among the Chinese population. Compared with the traditional detection methods, the detection method of the invention is fast, simple, practical, specific and sensitive, is suitable for the genetic identification of ABO blood groups of Chinese Han population and can be used in the research of the ABO blood group of human beings.

Description

[technical field] [0001] The invention relates to a new molecular biology detection reagent and method; in particular, it relates to a detection reagent and a typing method for synchronous genotyping of ABO blood group antigen and specific A2 antigen. [Background technique] [0002] ABO blood group is the blood group system with the strongest antigen immunogenicity in human blood group. The human ABO blood group system is controlled by the three alleles A, B and O on chromosome 9 (9q34.1~q34.2), and the gene is determined by determining the type of transglycosylase produced Which sugar group is catalyzed to be attached to which position of the precursor substance. Both A and B antigens are formed on the basis of H antigen (the precursor substance galactose is connected to fucose at the end), and the A enzyme synthesized by the cell can connect an acetylgalactosamine group to the H antigen to form the A antigen; B The antigen is a galactosyl group attached to the H antigen....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 邓志辉喻琼
Owner SHENZHEN BLOOD CENT
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