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Reagent and detection method capable of specifically detecting Mur antigen gene

Active Publication Date: 2015-02-25
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

[0005] At present, there are no genetic testing products specific for Mur antigen in the world or in China

Method used

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  • Reagent and detection method capable of specifically detecting Mur antigen gene
  • Reagent and detection method capable of specifically detecting Mur antigen gene
  • Reagent and detection method capable of specifically detecting Mur antigen gene

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Embodiment Construction

[0019] The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0020] Embodiment: The reagents involved are: the kit includes 250 μL of 2× master mix, 1 mL of RNase / DNase-free water, 25 μL of Mur upstream primer at a concentration of 10 μM / L, and 25 μL of a concentration of 10 μM / L L of Mur downstream primers, 25 μL of internal reference upstream primers at a concentration of 10 μM / L, 25 μL of internal reference downstream primers at a concentration of 10 μM / L, and 25 μL of positive controls.

[0021] The 2× master mix is ​​a product of Nearshore Protein Technology Co., Ltd., an...

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Abstract

The invention discloses a reagent and detection method capable of specifically detecting Mur antigen gene, the reagent is composed of 2 *premixed liquids, a Mur upstream primer, a Mur downstream primer, an internal reference upstream primer, an internal reference downstream primer, and DNA enzyme / RNA enzyme-free water, when in use, blood genome and the reagent are mixed for PCR (polymerase chain reaction) amplification reaction, PCR products are identified by electrophoresis for obtaining detecting results. According to the reagent and detection method capable of specifically detecting the Mur antigen gene, a simple, rapid, sensitive and specific PCR-SSP (polymerase chain reaction-sequence specific primer) method for detection of the Mur antigen gene in Miltenberger blood type is established by use of the Mur antigen gene specific primer, the method is accurate in detecting results, is conducive to large-scale and automated screening of the Mur antigen, can provide effective reference for clinical Mur antigen detection and identification and blood matching work, and ensures the safety of blood transfusion.

Description

technical field [0001] The invention relates to a molecular biology detection reagent and method, in particular to a specific gene detection reagent and method for Mur antigen in Miltenberger blood group system. Background technique [0002] In 1927, Landsteiner and Levine discovered the human MNS blood group. The number of polymorphisms in the antigens of this blood group system is second only to that of the Rh blood group system. The Miltenberger blood group system is a relatively rare subsystem in the MNS system. It is a group of variant antigens formed by the recombination and exchange of genes encoding glycophorin A (GPA) and glycophorin B (GPB). It consists of Mia, Vw, Eleven antigens, including Mur, Hil, Hut, MUT, Hop, Nob, DANE, TSEN and MINY, formed eleven different phenotypes. Among them, Mia and Mur, which often cause acute hemolytic transfusion reactions and hemolytic disease of the newborn, have received more and more clinical attention. [0003] Mur antigen (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6851C12Q2531/113C12Q2545/101
Inventor 魏双施李勇蒙青林王红梅段生宝田晶晶丁少华陈晔洲李冬
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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