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Reagent for detecting antiepileptic drug allergic reaction associated antigen genetype and clinical application method

A technology for antiepileptic drugs and allergic reactions, applied in the field of biomedicine, can solve problems such as complex procedures, high prices, and many detection sites, and achieve the effects of simple operation, low cost, and high accuracy

Inactive Publication Date: 2009-01-28
THE SECOND AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, due to the high price, there are many detection sites and complicated procedures when using this kit, it is not suitable for detecting antigens related to allergic reactions caused by antiepileptic drugs

Method used

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  • Reagent for detecting antiepileptic drug allergic reaction associated antigen genetype and clinical application method
  • Reagent for detecting antiepileptic drug allergic reaction associated antigen genetype and clinical application method
  • Reagent for detecting antiepileptic drug allergic reaction associated antigen genetype and clinical application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0039] 【Experimental Materials】

[0040] 1. Whole blood gDNA or gDNA from other tissues of patients taking antiepileptic drugs

[0041] 2. Main reagents

[0042] Taq enzyme (Dingguo), dNTPS, PCR buffer

[0043] 3. Primers and amplified target fragments

[0044] refer to Figure 1 to Figure 6, Antiepileptic drug-associated antigen genotype of sample detected by 2% gel, M: DNA marker; 1: carbamazepine-resistant patient 2: normal; 3: carbamazepine-SJS patient; 1, 2, 3 internal reference response C1 , C2 have specific target bands, and S4 reaction bands, and 3 also have S1, S2, S3 reaction bands, indicating that only carbamazepine-SJS patients are HLA-B*1502 genotype.

[0045] In this example, 4 pairs of sequence-specific primers were designed for exons 2, 3, and 4 of HLA-B*1502 (sequence number No. D50293); internal reference 1 was the amplification of intron 3 of HLA-DRB1 The product, internal reference 2 is the amplification product of exon 15 of the APC (adenomatous polyp...

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Abstract

The invention relates to a reagent used for detecting antigen genotypes relevant to the anaphylactic reaction of antiepileptic drugs, and a clinical application method thereof. The reagent is characterized in that: 1) the reagent comprises Taq enzyme, a chain of seven tubes and a PCR primer; 2) the PCR primer is shown in the sequence list SEQ ID No.1. The clinical application method is characterized in that: 1) four pairs of the sequence specific primers and two pairs of internal reference primers are designed by utilizing a sequence specific primer PCR-SSP according to an HLA-B sequence, and PCR amplification is carried out by taking gDNA extracted from human peripheral blood or other issues as a template; 2) gel electrophoresis is adopted for the detection so as to confirm that the genotype of the sample is HLA-B multiplied by 1502. The reagent and the method of the invention have the advantages of simple operation, high accuracy and low cost, and are especially suitable for determining that whether the antiepileptic drugs such as carbamazepine, etc. can be taken by the HLA-B multiplied by 1502 genotype detection before patients in China or Asia take antiepileptic drugs such as the carbamazepine, etc.

Description

technical field [0001] The invention relates to a reagent for detecting the genotype of an antigen related to an allergic reaction to an antiepileptic drug and a clinical application method thereof, which is a method for detecting the genotype of an antigen related to an allergic reaction to an antiepileptic drug by using PCR-SSP (sequence-specific primers) . It belongs to the technical field of biomedicine. Background technique [0002] Antiepileptic drugs are one of the common factors causing cutaneous adverse drug reactions (cADRs). cADRs range from mild maculopapular eruptions to severe life-threatening skin reactions such as Stevens-Johnson syndrome (SJS), toxic epidermal necrolysis (TEN), and drug hypersensitivity syndrome (HSS). Skin-type adverse reactions affect health, and the probability of severe life-threatening skin reactions is about 1:10,000, with a fatality rate of over 30%, among which the fatality rate of SCR / TEN is as high as 40%. More than 90% of sever...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 廖卫平石奕武高玫梅
Owner THE SECOND AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
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