Taqman probe fluorescent quantitation polymerase chain reaction (PCR) method for rapidly detecting pork or chicken compositions in food added with internal amplification control

A technique of amplifying internal standard and fluorescent quantification, which is applied to the rapid detection of chicken component Taqman probe fluorescent quantitative PCR and pork, to avoid false negative test results, facilitate operation, and reduce the risk of interference
CN102864243AActive Publication Date: 2013-01-09NANJING AGRICULTURAL UNIVERSITY
3 Cites 21 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2013-01-09

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a probe fluorescent quantitation polymerase chain reaction (PCR) method for rapidly detecting pork or chicken compositions in food added with internal amplification control. The method includes designing a primer and a probe respectively based on an animal nuclear gene; and artificially synthesizing one section of competitive internal amplification control and a corresponding probe,and establishing an internal standard fluorescent quantitation PCR system respectively, using ABI 7500 Software SDS 1.4 to analyze experiment results and taking amplification with a Ct value smaller than 36 as a detection positive result. According to the method, the method is provided with good specificity aimed at target species, false negative test results are avoided by monitoring PCR reaction in real time, a novel way is explored for identification of animal origin ingredients in food, and the method has the advantages of being accurate and stable, convenient to operate and the like.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of food safety detection, and relates to the rapid detection of animal-derived components in food, in particular to a Taqman probe fluorescence quantitative PCR rapid detection method for pork and chicken components in food added with amplified internal standards. Background technique

[0002] The adulteration of meat products is one of the focal issues of public concern. At present, meat adulteration is mainly manifested in the use of relatively cheap pork, chicken and other meat by unscrupulous companies, through various forms of deep processing, and selling them as beef products to seek improper benefits. Adulteration not only violates the legitimate rights and interests of consumers, but also greatly undermines the public's confidence in my country's food quality and safety. Therefore, it is necessary to establish a scientific, accurate and rapid detection method for pork and chicken, which are more commonly foun...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More