Probe for detecting matrilinear inheritance chondriosome deafness gene A1555G and its use

A deafness gene and mitochondrial technology, applied in the field of genetic engineering, can solve the problems of high price, cumbersome processing, and long time consumption, and achieve the effect of simplified agarose gel electrophoresis detection

Active Publication Date: 2007-06-27
金政策
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AI Technical Summary

Problems solved by technology

In foreign countries, restriction enzyme BsmAI (Alw26I) digestion combined with sequence analysis is widely used to identify the A1555G mutation of mtDNA. The enzyme digestion identification is convenient and fast, and the results are reliable. Compared with sex enzymes, the price difference is 50-100 times; at present, the mitochondrial gene A1555G mutation detection kit (patent number: ZL03156762.2) is mainly used in China. A new restriction enzyme site, and then d...

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  • Probe for detecting matrilinear inheritance chondriosome deafness gene A1555G and its use

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Embodiment Construction

[0041] Detection of mitochondrial gene A1555G mutation in maternally inherited deafness

[0042] 1. Test samples

[0043] 132 patients with sensorineural deafness were selected from the Deaf Disease Resource Bank of the Deaf Disease Molecular Diagnosis Center of the General Hospital of the Chinese People's Liberation Army, and peripheral whole blood DNA was extracted from the tested individuals (Yuan Huijun et al., Chinese Journal of Otorhinolaryngology, 1998, 33(2) : 67-70; Li Weimin et al., Journal of Clinical Otorhinolaryngology, 2001, 15 (Supplement): 53-58), as a test sample.

[0044] 2. Probe and Primer Design

[0045] According to the published mitochondrial gene sequence (Cambridge Sequence or NCBI human mitochondrial genome sequence NC-001807.4 or NT-006713.14, etc., or sequence number SEQ ID NO: 5), use the Genetool Lite program to assist in the design of primers and Taqman probe sequences, wherein:

[0046] The nucleotide sequence of the mutant fluorescent probe (...

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Abstract

The invention designs two pieces of Taqman mutant type and wild type probe and a pair of primer. Using Taqman probe method of real time fluorescence quantitation carries out genotype analysis for A1555G mutation of deaf gene of maternal inheritance mitochondria so as to diagnose genetic deaf disease of maternal mitochondria. The method is suitable to large-scale screening or preventative inspecting A1555G mutation of deaf mitochondria gene of maternal inheritance. Features are: simple, time saving, high specificity, high sensitivity, intuitive tested result, accurate and reliable.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, specifically a probe for detecting maternal mitochondrial genetic deafness and its use, more specifically a real-time fluorescent quantitative probe for detecting the mutation of maternal mitochondrial deafness gene A1555G and its use. The present invention also relates to the application of the probe and its related products in the preparation of kits or similar products for diagnosing maternally inherited mitochondrial deafness. Background technique [0002] Aminoglycoside antibiotics (streptomycin, gentamycin, kanamycin, tobramycin and micronomycin, etc.) are widely used clinically because of their broad-spectrum and high-efficiency antibacterial effects and low prices. Controls Gram-negative and positive bacterial infections, but these antibiotics have serious ototoxic side effects that can cause irreversible hearing loss in patients. In the past ten years, studies have found tha...

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Application Information

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IPC IPC(8): G01N33/52C12Q1/68
CPCC12Q2600/156C12Q1/6883
Inventor 戴朴袁永一韩东一金政策
Owner 金政策
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