Method for measuring content of ligustilide in ligusticum wallichii medical material through one-measurement multi-evaluation method

Abstract

The invention relates to a method for measuring the content of the ligustilide in a ligusticum wallichii medical material through a one-measurement multi-evaluation method. Pure substance psoralen reference substance which does not exist in a testing sample and is stable in performance and easy to obtain is adopted as an internal standard, the relative correction factor of the component and the ligustilide in the medical material is established, the content of ligustilide is calculated through the correction factor, and the liquid chromatography is adopted for carrying out measurement. The specific method comprises the following steps that firstly, chromatographic conditions and a system suitability test are made; secondly, mother liquor of the psoralen reference substance is prepared; thirdly, a solution of a test article is prepared; fourthly, measurement is carried out. Psoralen is taken as the mother liquor of the reference substance and is stable in performance and easy to obtain, the internal function relation and the proportion relation of the active ingredients of the traditional Chinese medicine are used, the ingredient content of the ligustilide can be measured directly, the measurement efficiency is improved, and operation is convenient.

Description

technical field [0001] The invention relates to the technical field of production and manufacture of pharmaceutical products, in particular to a method for measuring the content of ligustilide in Rhizoma Chuanxiong medicinal materials by adopting a method of one test and multiple evaluations. Background technique [0002] Ligusticum chuanxiong, that is, fragrant fruit, is a perennial herb of Umbelliferae and Ligusticum genus, 40-60 cm high. The rhizome is well developed, forming irregular nodular fist-shaped clumps. It is a kind of traditional Chinese medicine plant, which is often used for medicinal purposes. It functions to promote qi and relieve depression, expel wind and dampness, promote blood circulation and relieve pain, and treat headache, dizziness, rib pain and abdominal pain. , amenorrhea, dystocia, carbuncle sores and other diseases. [0003] Its main functional ingredient is volatile oil. At present, 40 components have been identified, accounting for 93.64% of ...

AI Technical Summary

Problems solved by technology

However, ligustilide is unstable at room temperature, and needs to be freeze-dried, sealed with nitrogen, stored in liquid nitrogen for long-term storage, and stored at a low temperature below -40°C before use, which is very difficult to handle

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