Detection method of phenolic acid compounds in compound radix salviae miltiorrhizae tablet

A compound Salvia miltiorrhiza tablet and a detection method technology, applied in the directions of measuring devices, instruments, scientific instruments, etc.

Inactive Publication Date: 2014-05-07
GUANGZHOU BAIYUSN HUTCHISON WHAMPOA CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Therefore, the purpose of the present invention is to provide a detection method for phenolic compounds in Compound Danshen Tablets, aiming at the deficiency that the content of phenolic acid compounds in Compound Danshen Tablets cannot be detected comprehensively, effectively and accurately at present. The method is simple and easy to operate, and can comprehensively, effectively and accurately detect the contents of phenolic acid compounds in Compound Danshen Tablets

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  • Detection method of phenolic acid compounds in compound radix salviae miltiorrhizae tablet
  • Detection method of phenolic acid compounds in compound radix salviae miltiorrhizae tablet
  • Detection method of phenolic acid compounds in compound radix salviae miltiorrhizae tablet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 The relative correction factor and standard relative of phenolic acid compounds to Danshensu sodium Determination of reserved value

[0065] Determined according to high performance liquid chromatography (Appendix VID of the Pharmacopoeia of the People's Republic of China, 2010 Edition).

[0066] Chromatographic conditions and system suitability test: Waters 2695 HPLC, Dionex UltiMate 3000 HPLC, Agilent 1260 HPLC; Merck Purospher STAR RP-18e (4.6mm×250mm, 5μm) column, Diamonsil C18 (2) (4.6mm×250mm, 5μm) chromatographic column, phenomenex luna C18 (2) (4.6mm×250mm, 5μm) chromatographic column, Waters Symmetry C18 (4.6mm×250mm, 5μm) chromatographic column, Waters SunFire C18 (4.6mm×250mm, 5μm) chromatographic column, Agilent TC-C18 (4.6mm×250mm, 5μm) chromatographic column, Ecosil C18 (4.6mm×250mm, 5μm) chromatographic column; use acetonitrile as mobile phase A, 0.3 vol% phosphoric acid Water is the mobile phase B, and the gradient elution is carried out a...

Embodiment 2

[0115] Example 2 Determination of phenolic acid compounds content in Compound Danshen Tablets

[0116] According to the specification requirements of Compound Danshen Tablets in the 2010 edition of Pharmacopoeia of the People's Republic of China, 18 batches of samples of Compound Danshen Tablets were taken for determination.

[0117] Determined according to high performance liquid chromatography (Appendix VID of the Pharmacopoeia of the People's Republic of China, 2010 Edition).

[0118] Chromatographic conditions and system suitability test: Waters 2695 high performance liquid chromatography; Waters Symmetry C18 (4.6mm×250mm, 5μm) chromatographic column; acetonitrile as mobile phase A, 0.3 volume % phosphoric acid water as mobile phase B, according to Table 8 Carry out gradient elution; detection wavelength is 230nm. The number of theoretical plates should not be less than 6000 based on the peak of Danshensu sodium.

[0119] Table 8 Gradient elution program

[0120]

...

Embodiment 3

[0157] Example 3 Determination of Phenolic Acids Content in Compound Danshen Tablets

[0158] According to the specifications of Compound Danshen Tablets in the 2010 edition of the Pharmacopoeia of the People's Republic of China, three batches of samples of Compound Danshen Tablets were taken for determination.

[0159] Determined according to high performance liquid chromatography (Appendix Ⅵ D of the Pharmacopoeia of the People's Republic of China, 2010 Edition).

[0160] Chromatographic conditions and system suitability test: Dionex U3000 high-performance liquid chromatography; Merck Purospher STAR RP-18e (4.6mm×250mm, 5μm) chromatographic column; acetonitrile as mobile phase A, 0.3 vol% phosphoric acid water as mobile phase B, Carry out gradient elution according to Table 14; detection wavelength is 225nm. The number of theoretical plates should not be less than 6000 based on the peak of Danshensu sodium.

[0161] Table 14 Gradient elution program

[0162]

[0163]...

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Abstract

The invention provides a detection method of phenolic acid compounds in a compound radix salviae miltiorrhizae tablet, and the detection method comprises the following steps: preparing a standard solution, a reference solution and a to-be-tested product solution, passing through a high performance liquid chromatography for detection; then calculating a relative correction factor of the phenolic acid compounds relative to salvianic acid A sodium; and using a formula IV shown in the specification too obtain the phenolic acid compound content in the compound radix salviae miltiorrhizae tablet. According to the detection method, by detection of one compound (salvianic acid A sodium), simultaneous determination of multiple compounds (salvianic acid A, protocatechuic aldehyde, rosmarinic acid, lithospermic acid and salvianolic acid B) can be realized, and the detection method is simple, rapid, accurate, good in universality, labor, and material and financial resource saving, and can more fully reflect the quality of the compound radix salviae miltiorrhizae tablet.

Description

technical field [0001] The present invention relates to the field of detection of pharmaceutical compounds. It specifically relates to a detection method for medicines, in particular to a detection method for phenolic acid compounds in compound Danshen tablets. Background technique [0002] Compound Danshen Tablets is a commonly used cardiovascular and cerebrovascular drug in clinical practice. It is a traditional Chinese medicine preparation made of Danshen, Panax notoginseng, and borneol as raw materials and auxiliary materials. It can promote blood circulation, remove blood stasis, regulate qi and relieve pain. Compound Danshen Tablets are recorded in the 2010 edition of the "Pharmacopoeia of the People's Republic of China" and are divided into three specifications. The first specification is film-coated small tablets, each weighing 0.32g, which is equivalent to 0.6g decoction pieces; the second specification is film-coated large tablets. Each piece weighs 0.8g, which is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 吴笛叶秋雄李楚源
Owner GUANGZHOU BAIYUSN HUTCHISON WHAMPOA CHINESE MEDICINE
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