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Method for extracting total flavonoid extract from desmodium styracifolium and method for measuring content of effective components in desmodium styracifolium

A technology of Guangshencao and active ingredients, which is applied in the field of biomedicine and can solve problems that need to be improved

Active Publication Date: 2015-11-25
WUHAN OPTICS VALLEY HUMANWELL BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the methods for detecting the content of various active ingredients schaftoside, Vicenin-1 and Vicenin-3 in Desmodium stylifolia medicinal materials, total flavonoids extracts from S. stylifolia and their preparations still need to be improved

Method used

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  • Method for extracting total flavonoid extract from desmodium styracifolium and method for measuring content of effective components in desmodium styracifolium
  • Method for extracting total flavonoid extract from desmodium styracifolium and method for measuring content of effective components in desmodium styracifolium
  • Method for extracting total flavonoid extract from desmodium styracifolium and method for measuring content of effective components in desmodium styracifolium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Instruments and reagents

[0043] WatersUPLCH-class ultra-performance liquid chromatograph; WatersACQUITYBEHC 18 (2.1mm×100mm, 1.7 microns); Shiseido CapellcoreC 18 (2.1mm×100mm, 2.7μm); Shimadzu Shim-packXR-ODSII (2.0mm×75mm, 2.2μm), CQ250 Ultrasonic Instrument (Shanghai Ultrasonic Instrument Factory); METTLER-Toronto XP205 Analytical Balance; Mettler - Toronto XS204 analytical balance; 9 controls are shown in Table 1 below.

[0044] Table 1 Reference substance information

[0045]

[0046]

[0047] All self-made reference substances were obtained from the liquid phase prepared by Waters, and phosphorus pentoxide was dried under reduced pressure for 12 hours before use; methanol was chromatographically pure, water was Millipore ultrapure water, and the rest of the reagents were of analytical grade.

Embodiment 2

[0048] Example 2 Sample

[0049] Ten different origins of Radix Radix are collected, respectively, to prepare 10 batches of total flavonoid extracts from Radix Radix. The information is shown in Table 2 below.

[0050] Table 2 Ten batches of total flavonoids extracts from Chlorella chinensis and their origins

[0051]

Embodiment 3

[0052] The investigation of embodiment 3 chromatographic conditions

[0053] 3.1 Selection of detection wavelength: Take appropriate amounts of the total flavonoids extract, Schifftanoside and the other eight self-made reference substances, dissolve them in 75% ethanol with ultrasonic waves, perform full-wavelength UV scanning at 210-400 nm, and conduct UV scanning at 270 nm and 270 nm. There is a maximum absorption at 330nm, but by comparing the chromatograms of the test product at these two wavelengths, it is found that the interference of impurities at 330nm is less, so 330nm is selected as the detection wavelength. UV scanning chromatogram see figure 1 , see the superimposed chromatograms of the test solution at 272nm and 330nm figure 2 .

[0054] of which in figure 1 Among them, 1 is the full-wavelength scanning chromatogram of DS-1, 2 is the full-wavelength scanning chromatogram of DS-3, 3 is the full-wavelength scanning chromatogram of DS-4, 4 is the full-wavelength...

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Abstract

The invention provides a method for measuring the content of vicenin-1, vicenin-3 and schaftoside in desmodium styracifolium medicinal materials, a total flavonoid extract from desmodium styracifolium and a preparation thereof at the same time by using a single reference substance, namely the schaftoside. The method comprises the steps that the schaftoside in total flavonoid extract from desmodium styracifolium or the preparation is taken as an internal standard substance, wherein the content of effective components of the schaftoside is high and easy to be obtained; the relative correction factor of the schaftoside and the vicenin-1 and the vicenin-3 is calculated, and the relative correction factor is taken as a constant, the content of the vicenin-1, the vicenin-3 and the schaftoside in the total flavonoid extract from desmodium styracifolium can be measured at the same time, the quality of the total flavonoid extract from desmodium styracifolium or the preparation in industrial manufacture can be scientifically, sensitively and quickly monitored, meanwhile the problem of the shortage of reference substances is overcome.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular, the invention relates to a method for extracting total flavonoids from Radix Radix et Rhizoma and a method for determining the content of active ingredients in Radix Radix et Rhizoma. Background technique [0002] Desmodium styracifolium (Osbeck) Merr. is a plant of the genus Desmodium styracifolium (Osbeck), the medicinal part of which is the dry aerial part. The main chemical components are flavonoids, saponins, polysaccharides, alkaloids and other compounds. It has the effect of clearing away heat and dehumidifying, diuretic and drenching stranguria. For hot stranguria, sand stranguria, stone stranguria, painful urination, edema, oliguria, jaundice, red urine, urinary tract stones. [0003] Schafposide, Vicenin-1 and Vicenin-3 are the active ingredients in Radix vulgaris, all belong to flavonoid carbon glycosides, and the content of these three components is relatively high. In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48G01N30/88
Inventor 王学海李莉娥许勇杨仲文黄天赐冯芸尹海龙黄璐杨婷余通杨成兵曹儒宾谢长
Owner WUHAN OPTICS VALLEY HUMANWELL BIO PHARMA
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