Method for extracting total flavonoids extract from Desmodium radix and method for determining content of active ingredients in Desmodium radix

A technology of wide variety of golden grass and active ingredients, applied in the field of biomedicine, can solve problems to be improved, etc.

Active Publication Date: 2019-11-01
WUHAN OPTICS VALLEY HUMANWELL BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the methods for detecting the content of various active ingredients schaftoside, Vicenin-1 and Vicenin-3 in Desmodium stylifolia medicinal materials, total flavonoids extracts from S. stylifolia and their preparations still need to be improved

Method used

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  • Method for extracting total flavonoids extract from Desmodium radix and method for determining content of active ingredients in Desmodium radix
  • Method for extracting total flavonoids extract from Desmodium radix and method for determining content of active ingredients in Desmodium radix
  • Method for extracting total flavonoids extract from Desmodium radix and method for determining content of active ingredients in Desmodium radix

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 instrument and reagent

[0043] Waters UPLC H-class ultra-high performance liquid chromatography; Waters ACQUITYBEH C 18 (2.1mm×100mm, 1.7 microns); Shiseido Capell core C 18 (2.1mm×100mm, 2.7 microns); Shimadzu Shim-pack XR-ODS II (2.0mm×75mm, 2.2 microns), CQ250 ultrasonic instrument (Shanghai Ultrasonic Instrument Factory); Mettler-Toronto XP205 analytical balance; Teller-Toronto XS204 analytical balance; 9 reference substances are shown in Table 1 below.

[0044] Table 1 Reference substance information

[0045]

[0046]

[0047] All self-made reference substances were obtained from the liquid phase prepared by Waters, and the phosphorus pentoxide was dried under reduced pressure for 12 hours before use; the methanol was chromatographically pure, the water was Millipore ultrapure water, and the rest of the reagents were analytically pure.

Embodiment 2

[0048] Example 2 sample

[0049] Ten batches of Desmodium stylifolia medicinal materials were collected from ten different origins, and ten batches of total flavonoids extracts of S.

[0050] Table 2 Ten batches of total flavonoids extracts of Desmodium stylifolia and their sources of origin

[0051]

Embodiment 3

[0052] The investigation of embodiment 3 chromatographic conditions

[0053] 3.1 Selection of detection wavelength: take appropriate amount of total flavonoids extract of Desmodium stylifolia, schiaftosides and the other eight self-made reference substances, respectively dissolve them in 75% ethanol by ultrasonic, conduct full-wavelength ultraviolet scanning at 210-400nm, and scan at 270nm and There is a maximum absorption at 330nm, but by comparing the chromatograms of the test product at these two wavelengths, it is found that the interference of impurities at 330nm is small, so 330nm is selected as the detection wavelength. For UV scan chromatogram see figure 1 , the superimposed chromatograms of the test solution at 272nm and 330nm are shown in figure 2 .

[0054] Among them in figure 1 Among them, 1 is the DS-1 full-wavelength scanning chromatogram, 2 is the DS-3 full-wavelength scanning chromatogram, 3 is the DS-4 full-wavelength scanning chromatogram, 4 is the DS-...

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Abstract

The invention provides a method for measuring the content of vicenin-1, vicenin-3 and schaftoside in desmodium styracifolium medicinal materials, a total flavonoid extract from desmodium styracifolium and a preparation thereof at the same time by using a single reference substance, namely the schaftoside. The method comprises the steps that the schaftoside in total flavonoid extract from desmodium styracifolium or the preparation is taken as an internal standard substance, wherein the content of effective components of the schaftoside is high and easy to be obtained; the relative correction factor of the schaftoside and the vicenin-1 and the vicenin-3 is calculated, and the relative correction factor is taken as a constant, the content of the vicenin-1, the vicenin-3 and the schaftoside in the total flavonoid extract from desmodium styracifolium can be measured at the same time, the quality of the total flavonoid extract from desmodium styracifolium or the preparation in industrial manufacture can be scientifically, sensitively and quickly monitored, meanwhile the problem of the shortage of reference substances is overcome.

Description

technical field [0001] The invention belongs to the field of biomedicine. Specifically, the invention relates to a method for extracting total flavonoids from Desmodium sativa and a method for measuring the content of active ingredients in Desmodium sativa. Background technique [0002] Desmodium styracifolium (Osbeck) Merr. is a plant of the leguminous genus Desmodium styracifolium (Osbeck) Merr. Its medicinal part is the dry aerial part, and its main chemical components are flavonoids, saponins, polysaccharides, alkaloids and other compounds. It has the effects of clearing away heat and dehumidification, diuresis and treating stranguria. It is used for hot shower, sand shower, stone shower, astringent and painful urination, edema and oliguria, jaundice, red urine, and urinary calculi. [0003] Schaftosides, Vicenin-1 and Vicenin-3 are active ingredients in Desmodium radix, all of which belong to flavonoid carbon glycosides, and the content of these three ingredients is re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48G01N30/88
Inventor 王学海李莉娥许勇杨仲文黄天赐冯芸尹海龙黄璐杨婷余通杨成兵曹儒宾谢长
Owner WUHAN OPTICS VALLEY HUMANWELL BIO PHARMA
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