Method for extracting total flavonoids extract from Desmodium radix and method for determining content of active ingredients in Desmodium radix
A technology of wide variety of golden grass and active ingredients, applied in the field of biomedicine, can solve problems to be improved, etc.
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Embodiment 1
[0042] Embodiment 1 instrument and reagent
[0043] Waters UPLC H-class ultra-high performance liquid chromatography; Waters ACQUITYBEH C 18 (2.1mm×100mm, 1.7 microns); Shiseido Capell core C 18 (2.1mm×100mm, 2.7 microns); Shimadzu Shim-pack XR-ODS II (2.0mm×75mm, 2.2 microns), CQ250 ultrasonic instrument (Shanghai Ultrasonic Instrument Factory); Mettler-Toronto XP205 analytical balance; Teller-Toronto XS204 analytical balance; 9 reference substances are shown in Table 1 below.
[0044] Table 1 Reference substance information
[0045]
[0046]
[0047] All self-made reference substances were obtained from the liquid phase prepared by Waters, and the phosphorus pentoxide was dried under reduced pressure for 12 hours before use; the methanol was chromatographically pure, the water was Millipore ultrapure water, and the rest of the reagents were analytically pure.
Embodiment 2
[0048] Example 2 sample
[0049] Ten batches of Desmodium stylifolia medicinal materials were collected from ten different origins, and ten batches of total flavonoids extracts of S.
[0050] Table 2 Ten batches of total flavonoids extracts of Desmodium stylifolia and their sources of origin
[0051]
Embodiment 3
[0052] The investigation of embodiment 3 chromatographic conditions
[0053] 3.1 Selection of detection wavelength: take appropriate amount of total flavonoids extract of Desmodium stylifolia, schiaftosides and the other eight self-made reference substances, respectively dissolve them in 75% ethanol by ultrasonic, conduct full-wavelength ultraviolet scanning at 210-400nm, and scan at 270nm and There is a maximum absorption at 330nm, but by comparing the chromatograms of the test product at these two wavelengths, it is found that the interference of impurities at 330nm is small, so 330nm is selected as the detection wavelength. For UV scan chromatogram see figure 1 , the superimposed chromatograms of the test solution at 272nm and 330nm are shown in figure 2 .
[0054] Among them in figure 1 Among them, 1 is the DS-1 full-wavelength scanning chromatogram, 2 is the DS-3 full-wavelength scanning chromatogram, 3 is the DS-4 full-wavelength scanning chromatogram, 4 is the DS-...
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