Quality evaluation method and application of wild chrysanthemum medicinal material

Abstract

The invention relates to a quality evaluation method and application of a wild chrysanthemum flower medicinal material. According to the method, a fingerprint spectrum of the wild chrysanthemum flowermedicinal material is constructed, the fingerprint spectrum is analyzed through a mode recognition method, and the qualitative and quantitative information of multiple components in the wild chrysanthemum flower medicinal material in a once-detection-multi-evaluation method is reflected comprehensively. The method comprises the following steps: preparing a mixed reference solution and a test solution, then respectively detecting the mixed reference substance solution and the test solution by adopting high performance liquid chromatography, recording fingerprint spectrums of the mixed reference substance solution and the test solution, recording the peak areas of the 12 components by adopting the once-detection-multi-evaluation method, calculating a relative correction factor, and calculating the contents of the 12 components by using the correction factor. According to the method, the contents of the 12 components in the wild chrysanthemum flowers are simultaneously determined by highperformance liquid chromatography, operation is simple and convenient, the reproducibility is high, multiple index components in the wild chrysanthemum flowers are quantified rapidly and accurately at the same time, the separation degree of the 12 components is high, and a theoretical basis is laid for quality control of the wild chrysanthemum flowers.

Description

technical field [0001] The invention relates to the field of medicinal material quality detection, in particular to a method for evaluating the quality of wild chrysanthemum medicinal material and its application. Background technique [0002] Wild chrysanthemum is the dry flower head of Chrysanthemum indicum L., a plant in the Compositae family. It is picked when the flowers open in autumn and winter, dried in the sun, or dried after steaming. It has the effect of evacuating wind-heat, reducing swelling and detoxification, and is used for treatment Boil carbuncle, sore throat, wind-fire red eyes, headache and dizziness and other diseases, organic acids and flavonoids are high in wild chrysanthemum medicinal materials, which have anti-inflammatory, anti-oxidation, anti-bacterial and other activities, of which caffeoylquinic acid Chrysanthemum chrysanthemum is widely used clinically, rich in resources, distributed in many provinces across the country, with significant differe...

AI Technical Summary

Problems solved by technology

[0002] Wild chrysanthemum is the dry flower head of Chrysanthemum indicum L., a plant in the Compositae family. It is picked when the flowers open in autumn and winter, dried in the sun, or dried after steaming. It has the effect of evacuating wind-heat, reducing swelling and detoxification, and is used for treatment Boil carbuncle, sore throat, wind-fire red eyes, headache and dizziness and other diseases, organic acids and flavonoids are high in wild chrysanthemum medicinal materials, which have anti-inflammatory, anti-oxidation, anti-bacterial and other activities, of which caffeoylquinic acid Similar structures, many isomeric compounds, expensive reference substances, wild chrysanthemums are widely used in clinical practice, rich in resources, distributed in many provinces across the country, with significant differences in growth environments, complex components, and difficult quality control and quality evaluation.

[0003] In the prior art, fingerprints can fully reflect the chemical composition information of Chinese medicinal materials, and have two attributes: completeness and fuzziness. The fuzziness leads to the lack of accurate qualitative and quantitative data support, which has certain limitations; The determination of index content can make up for the deficiency of fingerprint method in quality evaluation of traditional Chinese medicine and the limitation of single component content determination in quality evaluation to a certain extent. Traditional chromatographic peak identification requires a large number of reference substances as reference, and the more chromatographic peaks are identified, the more reference substances are required. Large, high research cost, external standard method content determination is inseparable from the reference substance, and the reference substance requires high purity, similar structure reference substances such as caffeoylquinic acid compounds are difficult to separate and purify, the price is expensive, which seriously hinders the identification of chromatographic peaks and Multi-index content determination

Images