Rapid qualitative and quantitative detection method for seven phenylethanoid glycoside components in medicinal material Herba Cistanche

Abstract

The invention discloses a rapid qualitative and quantitative detection method for seven phenylethanoid glycoside components in the medicinal material Herba Cistanche. The method can be used for determining the contents of active components in the medicinal material Herba Cistanche. The method comprises the following steps: with echinacoside as a reference substance, establishing relative calibration factors for echinacoside and verbascoside/isoacteoside/cistanoside C/cistanoside A/2'-acetylverbascoside/6'-acetylverbascoside by using a percent absorption coefficient; and then carrying out detection by using liquid chromatography, qualitatively determining the components according to relative retention time of each chromatographic peak and the peak of the reference substance, and quantitatively determining the components according to the peak areas of the components and the relative correction factors. Therefore, the method can simultaneously determine the contents of seven phenylethanoid glycoside components including echinacoside in the medicinal material Herba Cistanche by using only one reference substance, i.e., echinacoside.

Description

technical field [0001] The invention relates to the field of content determination of active ingredients of traditional Chinese medicines, in particular to a rapid qualitative and quantitative detection method for seven active ingredients of phenylethanol glycosides in Cistanche cistanche medicinal materials. Background technique [0002] Cistanche is a traditional kidney-tonifying traditional Chinese medicine in China. It is recorded in the 2015 edition of the Chinese Pharmacopoeia that it is the fleshy stem of Cistanche deserticola Y.C.Ma or Cistanche tubulosa (Schenk) Wight. It is mainly distributed in Inner Mongolia, Ningxia, Gansu, Qinghai and Xinjiang and other places. Cistanche and Cistanche have extremely high medicinal value. It enters the kidney and large intestine meridian. "Compendium of Materia Medica" records that Cistanche is "tonic but not harsh, so it has the name of Cistanche", "it is warm and can moisten and nourish Not dry, slippery and not diarrhea", ha...

AI Technical Summary

Problems solved by technology

At present, there is no report on the simultaneous quantitative determination of the seven phenylethanol glycosides active ingredients in Cistanche deserticola, especially the one-standard multi-test content determination method of Cistanche deserticola, which uses the percentage absorption coefficient to establish a relative correction factor, has not yet been reported in the literature or patented.

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