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Method for rapidly determining contents of EPA and DHA in cod liver oil

A rapid determination of cod liver oil technology, applied in the field of medicine, can solve the problems of cumbersome operation, expensive reference substances, poor accuracy of quantitative determination results, etc.

Inactive Publication Date: 2018-05-18
ZHOUSHAN INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the many shortcomings of the existing cod liver oil detection technology, and to design and propose a fast, simple, and environmentally friendly method for detecting EPA and DHA in cod liver oil, so as to solve the problems of cumbersome operation, expensive reference substances, and detection problems in the current detection method. Problems such as high cost, high toxicity of reagents, and poor accuracy of quantitative determination results

Method used

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  • Method for rapidly determining contents of EPA and DHA in cod liver oil
  • Method for rapidly determining contents of EPA and DHA in cod liver oil
  • Method for rapidly determining contents of EPA and DHA in cod liver oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] The detection method of the fatty acid in cod liver oil involved in this example comprises five steps of saponification reaction of test sample, methyl esterification reaction, salting-out extraction, dehydration, and gas chromatography sampling, and its specific detection process is:

[0099] (1) Saponification: Accurately weigh about 100 mg of the test product and put it in a 20 ml reaction bottle with a screw cap, add 1.5 ml of 2% sodium hydroxide methanol solution, tighten the bottle cap, (let the saponification proceed under airtight conditions, which can overcome Under higher temperature, methanol volatilizes and causes the reaction solvent to decrease, and the reaction rate decreases, thereby causing the fatty acid glyceride saponification to be incomplete or prolonging the saponification time.) Vortex mixing for 30 seconds, (vortex mixing can greatly increase the contact surface of the sample and the saponification reagent, and the reaction solution The color gra...

Embodiment 2

[0119] The detection method of the fatty acid in the shark liver oil involved in this example comprises five steps of saponification reaction of the test product, methyl esterification reaction, salting out extraction, dehydration, and gas chromatography sample injection, and its specific detection process is:

[0120] (1) Saponification: Accurately weigh about 100 mg of the test product and put it in a 20 ml reaction bottle with a screw cap, add 1.5 ml of 2% sodium hydroxide methanol solution, tighten the bottle cap, (let the saponification proceed under airtight conditions, which can overcome Under higher temperature, methanol volatilizes and causes the reaction solvent to decrease, and the reaction rate decreases, thereby causing the fatty acid glyceride saponification to be incomplete or prolonging the saponification time.) Vortex mixing for 30 seconds, (vortex mixing can greatly increase the contact surface of the sample and the saponification reagent, and the reaction solu...

Embodiment 3

[0140] The method for the detection of fatty acids in cod liver oil soft capsules involved in this example includes five steps of saponification reaction, methyl esterification reaction, salting-out extraction, dehydration, and gas chromatographic sample injection. The specific detection process is as follows:

[0141] (1) Saponification reaction: get 20 cod liver oil soft capsules, accurately measure its average loading, and mix the contents of 20 capsules evenly. Accurately weigh about 100 mg of the content and put it in a 20 ml reaction bottle with a screw cap, add 1.5 ml of 2% sodium hydroxide methanol solution, and tighten the bottle cap, (let the saponification reaction be carried out under airtight conditions, which can overcome the volatilization of methanol at higher temperatures) The reaction solvent is reduced, and the reaction rate is reduced, thereby causing incomplete saponification of fatty acid glycerides or prolonging the saponification time.) Vortex mixing for...

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Abstract

The invention relates to a method for rapidly determining the contents of omega-3 unsaturated fatty acids including EPA and DHA in cod liver oil. According to the method, EPA and DHA in cod liver oilis subjected to pre-column derivatization at first, the EPA and the DHA are determined by using gas chromatography and the contents of the EPA and the DHA in the cod liver oil are calculated by usinga correction factor-added internal standard method. The method comprises the following steps: 1, a pre-column derivatization reaction of the cod liver oil for preparation of a fatty acid methyl estersolution; 2, determination of relative correction factors of EPA methyl ester and DHA methyl ester; 3, gas chromatographic determination of the contents of the EPA and the DHA; and 4, calculation of the contents of the EPA and the DHA by using the correction factor-added internal standard method.

Description

technical field [0001] The invention belongs to the technical field of medicines, in particular to a method for rapidly measuring EPA and DHA in cod liver oil and cod liver oil soft capsules. Background technique [0002] Cod liver oil is a fatty oil extracted from fish liver mainly containing natural compounds such as vitamin A, vitamin D and various fatty acids, which are recorded in the pharmacopoeias of various countries. Cod liver oil contains more than 20 kinds of fatty acids, among which EPA and DHA are its unique fatty acids. EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) are omega-3 polyunsaturated fatty acids, which are almost absent in animals and plants on land, and have the highest content in fish liver. EPA and DHA are It is an indispensable and important nutrient for the human body, and its physiological activity is well known. At present, the domestic cod liver oil market is mixed, especially the quality of domestic pharmaceutical grade cod live...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/045
Inventor 王琼芬刘婷郑平安徐虹石婧
Owner ZHOUSHAN INST FOR FOOD & DRUG CONTROL
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