The invention provides a portable full-automatic
nucleic acid isothermal amplification device which comprises a device shell, the device shell is provided with a containing cavity capable of containing a micro-fluidic
chip, the containing cavity is provided with an inlet and outlet, a door
assembly is arranged at the inlet and outlet, a heating
assembly and a moving
assembly are arranged in the containing cavity, the heating assembly is used for providing constant-temperature heating for the micro-fluidic
chip, and the moving assembly is used for providing constant-temperature heating for the micro-fluidic
chip. The movement assembly comprises two lifting driving assemblies which are independent and controllable, and the two lifting driving assemblies can respectively drive a first
piston push rod and a second
piston push rod of the micro-fluidic chip to linearly move up and down in a one-to-one correspondence manner. Full-automatic operation of biological samples can be achieved, frequent manual operation of personnel is avoided, the hidden danger of inaccurate results caused by misoperation is effectively eradicated, meanwhile, the
stroke of the
piston push rod can be accurately mastered by driving the piston push rod through the movement assembly, the
control force is uniform and consistent, the
fluid pressure in the micro-fluidic chip is relatively balanced, and the working efficiency is improved. And bubbles are not easy to generate.