Portable full-automatic nucleic acid isothermal amplification device

Abstract

The invention provides a portable full-automatic nucleic acid isothermal amplification device which comprises a device shell, the device shell is provided with a containing cavity capable of containing a micro-fluidic chip, the containing cavity is provided with an inlet and outlet, a door assembly is arranged at the inlet and outlet, a heating assembly and a moving assembly are arranged in the containing cavity, the heating assembly is used for providing constant-temperature heating for the micro-fluidic chip, and the moving assembly is used for providing constant-temperature heating for the micro-fluidic chip. The movement assembly comprises two lifting driving assemblies which are independent and controllable, and the two lifting driving assemblies can respectively drive a first piston push rod and a second piston push rod of the micro-fluidic chip to linearly move up and down in a one-to-one correspondence manner. Full-automatic operation of biological samples can be achieved, frequent manual operation of personnel is avoided, the hidden danger of inaccurate results caused by misoperation is effectively eradicated, meanwhile, the stroke of the piston push rod can be accurately mastered by driving the piston push rod through the movement assembly, the control force is uniform and consistent, the fluid pressure in the micro-fluidic chip is relatively balanced, and the working efficiency is improved. And bubbles are not easy to generate.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chip nucleic acid constant temperature amplification equipment design, in particular to a portable fully automatic nucleic acid constant temperature amplification device. Background technique [0002] At present, many automated detection platforms have been developed at home and abroad, such as nucleic acid extractors, nucleic acid hybridization instruments, chemiluminescence instruments, etc. These automated detection platforms can only complete a certain step in the molecular diagnosis process in a laboratory environment, and few of them can A miniaturized portable detection platform that is directly applied to the on-site detection of pathogens. Although some foreign products such as GeneXpert and FilmArray have realized fully automated nucleic acid detection, their complex systems and high prices are not suitable for large-scale promotion. For highly infectious diseases, there is a great...

AI Technical Summary

Problems solved by technology

The patent document with the patent application number CN2020104472011 discloses an integrated self-service nucleic acid detection device, which is essentially a hand-held cartridge, which can be manually operated many times, so the operation is relatively cumbersome and the stroke of the piston push rod is difficult to accurately grasp; In addition, the control strength of manual operation is not uniform and the consistency is poor, which makes the internal fluid pressure of the microfluidic chip too high or too low, which is easy to generate air bubbles, and there is also a hidden danger of inaccurate results due to operational errors

Images