Method of Determination of Salvianolic Acid B and Schisandrin A in Yixin Fumai Granules by One Test and Multiple Evaluation
A technology of schisandrin A and salvianolic acid, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of rare reference substances, long inspection cycle, energy consumption, etc.
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Embodiment 1
[0146] Take Yixin Fumai Granules numbered as Test 1, and measure the contents of salvianolic acid B and schisandrin A.
[0147] Step 1: Preparation of Schisandrin A Reference Substance Solution
[0148] Take an appropriate amount of schisandrin A reference substance, accurately weigh it, add 70% methanol to make a solution containing 0.036 mg of schisandrin A per 1 ml, and obtain it.
[0149] Step 2: Preparation of Yixin Fumai Granules Test Solution
[0150] Take an appropriate amount of Yixinfumai granule and grind it finely, take about 1.0g, weigh it accurately, add 10ml of 70% methanol to dissolve it by ultrasound for 30min, take it out, place it at room temperature, make up the lost weight with 70% methanol, shake well, centrifuge, and Filter through a 0.45 μm microporous membrane, and take the filtrate.
[0151] Step 3: Inject the Schizandrin A reference substance solution and the test solution into the high-performance liquid chromatograph respectively, use octadecylsi...
Embodiment 2
[0155] Take the Yixin Fumai Granules numbered as Test 2, and measure the contents of salvianolic acid B and schisandrin A.
[0156] Step 1: Preparation of Schisandrin A Reference Substance Solution
[0157] Take an appropriate amount of schisandrin A reference substance, accurately weigh it, add 70% methanol to make a solution containing 0.030 mg of schisandrin A per 1 ml, and obtain it.
[0158] Step 2: Preparation of Yixin Fumai Granules Test Solution
[0159] Take an appropriate amount of Yixinfumai granule and grind it finely, take about 1.0g, weigh it accurately, add 5ml of 70% methanol to dissolve it by ultrasound for 20min, take it out, place it at room temperature, make up the lost weight with 70% methanol, shake well, centrifuge, and Filter through a 0.45 μm microporous membrane, and take the filtrate.
[0160] Step 3: inject the Schisandrin A reference substance solution and the test solution into the high-performance liquid chromatograph respectively, use high-pur...
Embodiment 3
[0164] Take Yixin Fumai Granules numbered as Test 3, and measure the contents of salvianolic acid B and schisandrin A.
[0165] Step 1: Preparation of Schizandrin A Reference Substance Solution
[0166] Take an appropriate amount of the reference substance of schisandrin A, accurately weigh it, add 70% methanol to make a solution containing 0.040 mg of schisandrin A per 1 ml, and obtain it.
[0167] Step 2: Preparation of the test solution
[0168] Take an appropriate amount of Yixinfumai granule and grind it finely, take about 1.0g, accurately weigh it, add 20ml of 70% methanol to dissolve it by ultrasound for 40min, take it out, place it at room temperature, make up the lost weight with 70% methanol, shake well, centrifuge, and Filter through a 0.45 μm microporous membrane, and take the filtrate.
[0169] Step 3: Inject the Schizandrin A reference substance solution and the test solution into the high-performance liquid chromatograph respectively, use octadecylsilane bonde...
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