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Chishui dendrobium nobile flower component content determination method

A technique for determination of Dendrobium officinale from Chishui, which is applied in the field of pharmacy, can solve problems such as incompleteness and weak characteristics, and achieves the effects of strong applicability, easy operation and quality assurance.

Active Publication Date: 2022-02-11
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are few studies on the specific chemical components contained in Dendrobium nobile flowers, and there is only one fingerprint patent on the quality control method at present, which only refers to the component D-anhydroglucose, which is not very characteristic and is not yet available. Not comprehensive, in-depth research is necessary

Method used

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  • Chishui dendrobium nobile flower component content determination method
  • Chishui dendrobium nobile flower component content determination method
  • Chishui dendrobium nobile flower component content determination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, Chishui Dendrobium nobile flower HPLC assay method

[0034] 1 Materials and methods

[0035] 1.1 Instrument

[0036] Agilent 1260 Infinity high performance liquid chromatograph, equipped with G1315D variable wavelength detector (Agilent, USA); KQ-300VDE dual-frequency numerical control ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); Waterpur ultrapure water equipment (Sichuan Wotel Water Treatment Equipment Co., Ltd.); SpectraMax Plus 384 microplate reader (Meigu Molecular Instrument Co., Ltd.); 1 / 100,000 electronic balance (Sartorius Scientific Instrument Co., Ltd.); electronic balance (Shanghai Jinghai Instrument Co., Ltd. Company); constant temperature water bath (Hangzhou Youning Instrument Co., Ltd.).

[0037] 1.2 Reagent

[0038] Rutin (mass fraction>98%, batch number AF9022006), liquiritin (mass fraction>98%, batch number AF8111195), kaempferol-3-glucose rhamnoside (mass fraction>98%, batch number AF20050831), all purchased from Ch...

Embodiment 2

[0067] Embodiment 2, optimization of Chishui Dendrobium nobile flower chromatographic conditions and sample processing method

[0068] The Agilent Eclipse Plus C 18 Column (150 mm×4.6 mm, 5 μm), ThermoAcclaim TM 120C 18 Column (150 mm × 4.6 mm, 5 μm) and Agilent Eclipse XDB-C 18 Column (150 mm×4.6mm, 5 μm) three chromatographic columns and three detection wavelengths of 254, 280, 360 nm, the results show that Agilent Eclipse PlusC 18 The column (150 mm×4.6 mm, 5 μm) has better column efficiency, and the sample solution has a better peak shape at 280 nm. In addition, different mobile phase systems such as acetic acid water-methanol, acetic acid water-acetonitrile, phosphoric acid water-methanol and phosphoric acid water-acetonitrile were investigated in the experiment. The results showed that the peak eluting time was the most suitable under the mobile phase system of 0.06% acetic acid water-acetonitrile , the characteristic peaks can meet the resolution requirements, the ...

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Abstract

The invention discloses a Chishui dendrobium nobile flower component content determination method which comprises the following steps: S1, preparation of a reference substance solution: respectively precisely weighing liquiritin, kaempferol-3-glucose rhamnoside and an isoquercitrin standard substance, and dissolving in a solvent to obtain a mixed reference substance solution; s2, preparing a Chishui dendrobium nobile flower test solution; and S3, content determination: determining by adopting a high performance liquid chromatography. According to the determination method, the contents of three chemical components including liquiritin, kaempferol-3-glucose rhamnoside and isoquercitrin in the dendrobium nobile lindl flowers are determined, and the quality of the Chishui dendrobium nobile lindl flowers can be better controlled.

Description

technical field [0001] The invention relates to the technical field of pharmacy, in particular to a method for determining the composition content of Dendrobium nobile flowers. Background technique [0002] Dendrobium nobile is a member of the genus Dendrobium orchidaceae ( Dendrobium nobile Lindl.), which is one of the three main varieties recorded in the 2020 edition of Chinese Pharmacopoeia Dendrobium. "Shen Nong's Materia Medica" records that "Dendrobium nobile mainly treats the wound, removes numbness, lowers qi, nourishes the five internal organs, wins thinness, and strengthens yin. Take it for a long time to thicken the stomach. Lighten the body and prolong life." It is believed that it is beneficial to the stomach and promotes body fluid, nourishes yin and clears heat. and other effects. [0003] Dendrobium nobile is mainly distributed in Guizhou, Sichuan, Yunnan, Guangxi, Hainan, Taiwan and other places in China, and Chishui, Guizhou is the best, and it is "authe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 段灿灿田鑫张建永李尧冯文忠
Owner ZUNYI MEDICAL UNIVERSITY
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