The invention relates to a liquid chromatography-mass spectrometry determination method for a Holdan tablet. The method comprises the following steps: mixing psoralen glycoside, isopsoralen glycoside, hyperoside, quercetin 3-O-glucuronide, isoquercitrin, sennoside B, astragalus smicus glycoside, sennoside A, rosmarinic acid, alkannic acid, salvianolic acid B, nuciferine, salvianolic acid A, psoralen, isopsoralen, salvianolic acid C, rhein, neopsoralen isoflavone, psoralen and cryptotanshinone, and uniformly stirring to obtain a mixture; the method comprises the following steps of: preparing reference substance solutions A and B and a test sample solution by taking a sample as a reference substance and bakuchiol as a reference substance, injecting the reference substance solutions A and B and the test sample solution into a liquid chromatograph-mass spectrometer, obtaining mass spectrograms when A is in a positive ion mode and B is in a negative ion mode, and calculating the content of the effective components in the test sample according to the mass spectrograms under the chromatographic conditions that an Agilent C18 chromatographic column (4.6 mm * 250 mm, 5 [mu] m), the flow rate is 0.4 mL.min <-1 > and the sample injection volume is 2 [mu] L. The column temperature is 30 DEG C, and a 0.2% phosphoric acid aqueous solution (A)-acetonitrile (B) is used as a mobile phase for gradient elution.