23 results about "Isoquercitroside" patented technology

The application discloses a kind of cydia pomella larva's food lure composition and application thereof.It includes the following weight parts of component: isoquercitrin 0.02~0.07 parts, phlorizin 0.6~0.9 parts, cyanidin-3-O-galactoside 0.01~0.02 parts, delphinidin-3-O-galactose 0.05~0.12 parts.The food lure component provided in the application has low dosage and low price, can improve the behavior regulation effect of lure core on larvae, reduce the production cost, and is suitable for popularization and promotion.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a method for determining the content of five flavonoid components in a hypericum perforatum medicinal material. The content determination method comprises the following steps: preparing rutin, hyperoside, isoquercitrin, quercitrin and quercetin into a mixed reference solution; extracting a hypericum perforatum medicinal material by using an extraction solvent to prepare a test solution; the mixed reference substance solutions and the test solution with different volumes are subjected to high performance liquid chromatography respectively, a mobile phase A is acetonitrile, a mobile phase B is a phosphoric acid aqueous solution with the volume concentration of 0.08-0.12%, and elution gradient is adopted. The content determination method has the advantages of high sensitivity, simplicity and convenience in operation, low cost and high detection speed.

The invention discloses an application of isoquercitrin in preparation of a medicine for improving insulin resistance and diabetes-related liver diseases, and belongs to the technical field of medicines. According to the application, a high-glucose and high-fat diet and streptozotocin combined method is adopted to successfully construct a type 2 diabetes mouse model; experiments find that the isoquercitrin can effectively relieve the symptoms of weight loss and water intake increase of T2DM mice, significantly reduce the fasting blood-glucose level and improve abnormal glucose tolerance; the isoquercitrin can reduce the liver index of a T2DM mouse, relieve fatty degeneration of the liver and repair a liver cell structure; by activating a PI3K / AKT signal channel, the isoquercitrin up-regulates the expression levels of PI3K, p-AKT, p-GSK3 and GLUT4 and down-regulates the expressions of GSK3 and PEPCK at the same time, so that the insulin resistance of T2DM mice is effectively improved, the fatty degeneration of the liver is improved, and the isoquercitrin has a protection or repair effect on liver injury.

The present invention discloses a method for screening effective substances of traditional Chinese medicine based on a 6-dimensional spider web model, belonging to the field of medical technology. Based on the results of UHPLC analysis, the present invention uses danshensu, chlorogenic acid, procyanidin B2, epicatechin, hyperoside, isoquercetin, rosmarinic acid, lithospermic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone I, tanshinone I, cryptotanshinone, tanshinone IIA, oleanolic acid, and ursolic acid as the "specific" candidate effective ingredients of the Danshen-Hawthorn medicinal pair. Based on the data of the candidate effective ingredients in six dimensions, namely, effectiveness dimension, compatibility environment dimension, transmission and traceability dimension, content measurability dimension, network pharmacology degree value dimension, and molecular docking target binding activity dimension, a "spider web" model is constructed to obtain the key effective substances of the Danshen-Hawthorn medicinal pair. The present invention provides theoretical support for revealing the compatibility theory of the Danshen-Hawthorn medicinal pair, formulating its quality evaluation standards, and clinical application of the Danshen-Hawthorn medicinal pair.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

The invention provides a construction method of the UPLC specific chromatogram of sword bean and preparation thereof, which comprises the following steps: A) dissolving a test raw material with a solvent, and extracting to obtain a to-be-detected solution; b) determining the liquid to be detected by adopting a high performance liquid chromatography to obtain the UPLC characteristic chromatogram of the sword bean and the preparation thereof; chromatographic conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 column; a mobile phase A is an acetonitrile solution, a mobile phase B is a 0.1% phosphoric acid aqueous solution, and gradient elution is carried out. According to the method, a high performance liquid chromatography is adopted, an acetonitrile-0. 1% phosphoric acid solution is selected as a mobile phase for gradient elution, quercetin-3-O-glucose-7-O-rhamnoside, myricetin-3-O-galactoside, lysimachin, rutin and isoquercitrin are taken as reference substances, and the UPLC characteristic spectrum method of the sword beans and the preparation thereof is established. And more scientific technical means are provided for controlling the medicinal quality of the sword beans and the preparation thereof.

The invention discloses application of a herba houttuyniae extract in preparation of a grouper iridovirus inhibitor. The herba houttuyniae extract is obtained by extracting a herba houttuyniae sample with water or an organic solvent. The herba houttuyniae extract prepared by the invention has a good inhibition effect on grouper iridovirus, does not generate drug resistance, has the characteristics of high efficiency, safety and no toxic or side effect, has a better antiviral effect compared with quercetin and isoquercitrin pure compounds, and has a wide application prospect in aquaculture.

The invention relates to application of enzyme modified isoquercitrin. The present invention provides a salivary secretion stimulator having a dosage form usable in the oral cavity or a dosage form usable by oral administration (dosage form for oral cavity, dosage form for oral administration). The salivary secretion stimulating agent may be prepared using an enzyme modified isoquercitrin or a combination of an enzyme modified isoquercitrin and a thickening polysaccharide. The invention also provides an additive (an additive for oral compositions), the composition is effective for preparing an oral composition (a food or beverage, pharmaceutical product for oral administration) and can impart at least one activity selected from the group consisting of salivary secretion stimulating activity, swallowing improving activity (swallowing assisting activity) and chewing improving activity (chewing assisting activity) to the oral composition. The additive may be prepared using an enzyme modified isoquercitrin in combination with a thickening polysaccharide.

The invention provides a method for detecting HPLC (high performance liquid chromatography) characteristic chromatograms of senecio scandens medicinal materials, decoction pieces, standard decoction and formula granules, which comprises the following steps: pre-treating raw materials of a test sample to obtain liquid to be detected; the pretreatment mode comprises solvent dissolution and extraction; measuring the liquid to be measured by adopting HPLC (High Performance Liquid Chromatography) to obtain HPLC characteristic chromatograms of the senecio scandens medicinal material, decoction pieces, standard decoction and formula granules; the preparation method comprises the following steps: preparing a reference substance solution, namely dissolving neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol by adopting a solvent; obtaining a reference substance solution of the reference substance; the HPLC conditions are as follows: a chromatographic column is a phenyl column; a mobile phase A is an acetonitrile solution, a mobile phase B is a 0.3% phosphoric acid aqueous solution, and gradient elution is carried out; according to the method, a high performance liquid chromatography method is adopted, and an acetonitrile-0. 3% phosphoric acid solution is selected as a mobile phase for gradient elution; the method comprises the following steps: taking neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol as reference substances; the HPLC characteristic chromatogram method of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules is established, and more scientific technical means are provided for controlling the medicinal quality of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules.

There are described compositions comprising an effective amount of a combination of two or more components, said components selected from acacetin, ACTI peptide, alpha-lipolic acid, alprostadil, anisomycin, apigenin, ascorbic acid, astragalus, berberine, β-lapachone, β-hydroxy-beta-methyl-butyrate, Bacopa monnieri, catechin, catechol, chamomile, chrysin, coumestrol, curcumin, dinitrophenol, dinoprost, ellagic acid, (−)-epigallocatechin gallate, green tea extract, fisetin, genistein, ginsenoside RE, glabridin, 18-α-glycyrrhetinic acid, 18-β-glycyrrhetinic acid, glycyrrhizin, hydroquinone, isoquercitrin (EMIQ), kaempferol, kuromanin, leucine, lithium, luteolin, luteolin, luteolinidin, melatonin, menadione, 1-methylnicotinamide (MNA), methyl salicylate, myricetin, nadide, niacin (vitamin B3), nicotinamide (NAM), nicotinamide mononucleotide (NMN), nicotinamide riboside (NR), nicotinic acid adenine dinucleotide (NaAD), nicotinic acid mononucleotide (NaMN), parsley (Petroselinium crispum), phenylephrine, pokeweed mitogen, 15-Δ prostaglandin J2, puromycin, quercetin, quinolinic acid, retinoic acid, trichostatin A, troxrutin, rutin, tryptophan, vitamin D3, withaferin A, wortmannin and zinc (including salts thereof).

The invention discloses an extraction method of a salvia miltiorrhiza-hawthorn medicine pair, and belongs to the technical field of traditional Chinese medicine extraction. According to the extraction method disclosed by the invention, the total content of tanshinol, chlorogenic acid, procyanidine B2, epicatechin, hyperoside, isoquercitrin, alkannic acid, rosmarinic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone and tanshinone IIA is taken as an extraction index, ethanol is taken as an extraction solvent, and the salvia miltiorrhiza-hawthorn medicine pair is subjected to reflux extraction. The process parameters in the extraction method are optimized by combining a single factor test with a Box-Behnken response surface method, and the salvia miltiorrhiza-hawthorn drug pair extract with the effective component content not lower than 8.0% is obtained. Based on a multi-index evaluation standard, a process with a better extraction effect is provided for development and application of the salvia miltiorrhiza-hawthorn medicine pair.

The invention provides a method for preparing high-purity isoquercitrin from polygonum viviparum, which comprises the following steps: sequentially carrying out water extraction impurity removal and alcohol extraction on a polygonum viviparum raw material to obtain an alcohol extract; decolorizing the alcohol extract, then adjusting the pH value to 9-11 to precipitate and remove tannin, and then carrying out vacuum concentration to obtain a general flavone crude product; performing acid hydrolysis on the crude product, performing separation and purification by adopting high-pressure preparative liquid chromatography, performing online monitoring by utilizing an ultraviolet detector, and obtaining a fraction rich in isoquercitrin by accurately controlling starting and ending points of fraction collection; and concentrating under reduced pressure, washing with water and drying to obtain the product. By optimizing the pretreatment and chromatographic separation conditions, the purity and yield of the product are synchronously improved, the HPLC (High Performance Liquid Chromatography) purity of the obtained isoquercitrin reaches 98% or above, and the method is low in process operation cost, environment-friendly and suitable for large-scale production.

The amino acid sequence of the novel thermophilic alpha-L-rhamnosidase is shown as SEQ ID No.1, and the novel thermophilic alpha-L-rhamnosidase is derived from a metagenome of a hot spring in Yuanjiang County in Yunnan province. The thermophilic alpha-L-rhamnosidase YJ-Rha72 provided by the invention can hydrolyze flavonoid substances such as rutin, naringin, hesperidin, neohesperidin and the like and p-nitrophenyl-alpha-L-rhamnopyranoside (pNPR), the rutin hydrolysis activity is the highest, and the thermophilic alpha-L-rhamnosidase YJ-Rha72 has the hydrolytic activity of alpha-1, alpha-1, 2 and alpha-1, 6 glucosidic bonds. The optimum reaction temperature of the enzyme is 75 DEG C, and the activity of the enzyme at 50-85 DEG C exceeds 34.9%; the optimum reaction pH is 6.0, and the activity exceeds 74% in the pH range of 5-7.5. The enzyme can completely hydrolyze 1 mM of rutin within 20 minutes, and the conversion rate is 100%. The alpha-L-rhamnosidase YJ-Rha72 has important application potential and value, for example, the alpha-L-rhamnosidase YJ-Rha72 is used for preparing important bioactive substances such as isoquercitrin and Prunin through biotransformation of rhamnoside substances, debitterizing citrus juice, enhancing aroma of grape wine and the like.

The present invention relates to a method for preparing isoquercetin that inhibits xanthine oxidase activity, characterized by comprising the following steps: (1) using dried tangerine peel as a raw material, extracting it with an organic liquid, combining the extracts, and draining the solvent using a rotary evaporator to obtain an organic layer extract AE; (2) separating and purifying the organic layer extract AE through a column to obtain component AE-3; (3) separating and purifying component AE-3 again to obtain component AE-3-7; and identifying component AE-3-7 as containing isoquercetin by nuclear magnetic resonance; and (4) conducting an enzyme activity inhibition experiment. The preparation process of the present invention is simple and easy to operate; the obtained isoquercetin is extracted, separated and purified from dried tangerine peel, and is found to have the ability to inhibit xanthine oxidase activity, providing a new source for the prevention and treatment of hyperuricemia, and having great significance for the research on the effective ingredients of natural products of Chinese medicinal materials with medicinal and edible properties.

The present invention provides a saliva secretion stimulating agent having a dosage form usable in the oral cavity or a dosage form usable by oral administration (dosage form for the oral cavity, dosage form for oral administration). The saliva secretion stimulating agent can be prepared using enzyme-modified isoquercitrin or a combination of enzyme-modified isoquercitrin and a thickening polysaccharide. The present invention also provides an additive (additive for oral composition) that is effective for preparing an oral composition (food or beverage, pharmaceutical product for oral administration) and can impart at least one activity selected from the group consisting of saliva secretion stimulating activity, swallowing improvement activity (swallowing aid activity), and mastication improvement activity (mastication aid activity) to the oral composition. The additive can be prepared using a combination of enzyme-modified isoquercitrin and a thickening polysaccharide.

The invention discloses a method for detecting the content of isoquercitrin in a chingma abutilon seed sample and application of the method. A test solution and a reference solution are prepared respectively and injected into an ultra-high performance liquid chromatograph, and specific chromatograms of the reference solution and the test solution are compared; and calculating the content of the isoquercitrin in the chingma abutilon seed sample by utilizing a regression equation of the sample size and the peak area of the isoquercitrin. The method disclosed by the invention is good in specificity and durability, and a new analysis means can be provided for quality detection of a chingma abutilon seed sample; the specific component of isoquercitrin is taken as an index, so that the method is more reasonable and accurate; the method is applicable to detection of chingma abutilon seed medicinal materials, standard decoction, formula granules and other products containing chingma abutilon seeds. The method is simple to operate, good in reproducibility and stability, reliable in recovery rate, low in detection cost, high in detection efficiency and small in environmental pollution.

This invention discloses a method for determining the quality of *Hemiberlesia lingua* stems and leaves. The method includes fingerprint spectroscopy establishment and similarity analysis, content determination, cluster analysis, principal component analysis, and partial least squares discriminant analysis. The method of this invention involves UPLC fingerprint analysis and similarity analysis of 15 batches of *Hemiberlesia lingua* stems and leaves from different origins. A total of 28 common peaks were found, and 7 of these common peaks were identified using reference standards: peak 4 (neochlorogenic acid), peak 8 (chlorogenic acid), peak 9 (caffeic acid), peak 11 (cryptochlorogenic acid), peak 22 (isoquercetin), peak 24 (astragalin), and peak 28 (isochlorogenic acid C). This invention's method is highly sensitive, specific, stable, and feasible, providing a reference for the quality evaluation and control of *Hemiberlesia lingua* stems and leaves.

The present application relates to the application of isoquercitrin in the preparation of a drug for treating post-traumatic stress disorder, and belongs to the technical field of natural chemical medicine development. The present application systematically clarifies the whole-chain mechanism of isoquercitrin in treating PTSD through the "GABA A R-PINK1 / Parkin-mitophagy" pathway by means of a variety of technical means such as molecular docking, microthermal swimming, patch clamp, proteomics, cell and animal models. Compared with the clinically commonly used drugs for treating post-traumatic stress disorder, isoquercitrin, as a natural flavonoid compound, can improve the symptoms of PTSD while not damaging the learning and memory function, and has better safety. Moreover, isoquercitrin is widely sourced and has a mature preparation process. The present application provides a theoretical basis and experimental support for the application of isoquercitrin in the development of drugs for treating post-traumatic stress disorder, and has important clinical application value and industrialization prospects.

The present application relates to the technical field of traditional Chinese medicine analysis, and particularly relates to a mulberry fruit paste fingerprint spectrum establishment method and fingerprint spectrum. The mulberry fruit paste fingerprint spectrum establishment method comprises the following steps: taking mulberry fruit paste as raw material to prepare a test sample solution, taking chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, 5-hydroxymethyl furfural, rutin and isoquercitrin to prepare reference sample solutions; injecting the test sample solution and the reference sample solutions into an ultra-high performance liquid chromatograph for determination, recording chromatograms, collecting chromatograms of test sample solutions of multiple batches of mulberry fruit paste, importing a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, generating a control spectrum, and taking peak area and retention time as parameters to calculate similarity. The method can simultaneously detect multiple components in mulberry fruit paste, and can be effectively used to evaluate product quality stability of mulberry fruit paste.

The invention discloses an application of a pyracantha fortuneana fruit extract in prevention and treatment of ulcerative colitis, and relates to the technical field of food medicines. The main components of the pyracantha fortuneana fruit extract comprise total flavonoids, total polyphenols and total triterpenes. Wherein the total flavonoids and the total polyphenols comprise hyperoside, rutin, isoquercitrin and epicatechin, and the total triterpenes comprise jujubogenin and the like. Functional food, health care products, medicines and other products prepared from the pyracantha fortuneana fruit extract can reduce the disease activity index, increase the colon length, reduce the abnormally-increased pro-inflammatory cytokine TNF-alpha content in serum, improve the glutathione peroxidase activity and achieve prevention and treatment of ulcerative colitis. Therefore, the pyracantha fortuneana fruit extract disclosed by the invention can be used as a raw material for preparing foods and medicines for preventing and treating ulcerative colitis.

The invention discloses a method for producing a flos carthami extract with high isoquercitrin content, which comprises the following steps: 1) extracting flos carthami in a fading period by using methanol, ethanol and / or water; and 2) drying the extracting solution. Research on differential metabolites and differential genes in petals in different flowering stages shows that the content of effective component flavone in safflower in a fading stage is lower than that in safflower in a full-bloom stage, the petal metabolites and genes in the three stages are obviously separated, and samples in the initial flowering stage and the full-bloom stage are close to each other and far away from samples in the fading stage. The content of isoquercitrin in petals in the fading period of the extract extracted by the method is high and can reach 0.164 mg / g, and the content of isoquercitrin in the full-bloom period can reach 0.067 mg / g. The safflower extract with high isoquercitrin content is produced by adopting the fading period safflower, so that the fading period safflower is turned into wealth, and the economic benefit is increased.

The invention discloses a rapid analysis and content determination method for components of an alcohol extract of sedum emarginatum, relates to the technical field of quality control of medicinal materials, and solves the problem that the detection efficiency is low when the contents of various components of sedum emarginatum are determined in the prior art. According to the method, under the same chromatographic condition, six components including schaftoside, isoquercitrin, astragalus smicus glycoside, p-coumaric acid, gallic acid and protocatechuic acid contained in the sedum emarginatum alcohol extract are subjected to high performance liquid chromatography determination at the same time, and the problem that the detection efficiency is low when the content of various components of sedum emarginatum is determined at present is solved. The content of various components such as sedum emarginatum schaftoside, isoquercitrin, astragalus smicus glycoside, p-coumaric acid, gallic acid and protocatechuic acid can be determined under the same high performance liquid chromatography condition, and the determination method is accurate, high in sensitivity, good in repeatability and high in stability.

The invention belongs to the technical field of medicinal material identification, and particularly relates to a method for identifying polygonum viviparum rhizomes and adulterants thereof, and the method for identifying the polygonum viviparum rhizomes and adulterants thereof comprises the step of identifying the polygonum viviparum rhizomes and adulterants thereof by detecting the contents of rutin and isoquercitrin in a rhizome sample to be detected. According to the identification method established by the invention, the polygonum viviparum rhizomes and the four polygonum viviparum rhizome adulterants are identified according to the contents of rutin and isoquercitrin in the rhizome sample to be detected, and a scientific basis is provided for quality evaluation and improvement of the polygonum viviparum rhizomes.