35 results about "Isoquercitroside" patented technology

The invention discloses application of a glucosyl modified quercetin derivative and a composition containing the glucosyl modified quercetin derivative. Specifically, the invention discloses an application of glucosyl modified quercetin as an anti-skin photoaging agent. According to the application disclosed by the invention, glucosyl modified quercetin, especially glucosyl modified isoquercitrin, has a remarkable effect in the aspect of resisting skin photoaging; cosmetics prepared from glucosyl modified quercetin have good anti-aging ability, and can significantly improve percutaneous water loss, skin elasticity and skin roughness.

The application discloses a kind of cydia pomella larva's food lure composition and application thereof.It includes the following weight parts of component: isoquercitrin 0.02~0.07 parts, phlorizin 0.6~0.9 parts, cyanidin-3-O-galactoside 0.01~0.02 parts, delphinidin-3-O-galactose 0.05~0.12 parts.The food lure component provided in the application has low dosage and low price, can improve the behavior regulation effect of lure core on larvae, reduce the production cost, and is suitable for popularization and promotion.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a method for determining the content of five flavonoid components in a hypericum perforatum medicinal material. The content determination method comprises the following steps: preparing rutin, hyperoside, isoquercitrin, quercitrin and quercetin into a mixed reference solution; extracting a hypericum perforatum medicinal material by using an extraction solvent to prepare a test solution; the mixed reference substance solutions and the test solution with different volumes are subjected to high performance liquid chromatography respectively, a mobile phase A is acetonitrile, a mobile phase B is a phosphoric acid aqueous solution with the volume concentration of 0.08-0.12%, and elution gradient is adopted. The content determination method has the advantages of high sensitivity, simplicity and convenience in operation, low cost and high detection speed.

The invention belongs to the technical field of traditional Chinese medicine analysis, and particularly relates to a method for detecting various flavonoid components in cottonrose hibiscus leaf medicinal materials. The method comprises the following steps: detecting a test sample by adopting HPLC (High Performance Liquid Chromatography) to obtain a high performance liquid chromatography detection result of flavonoid components in the test sample; the test sample is a cotton rose hibiscus leaf medicinal material extraction solution, and the flavonoid components comprise at least four of rutin, isoquercitrin, kaempferol-3-O-beta-robinia bioside, kaempferol-3-O-rutinoside, kaempferol-3-O-beta-D-glucoside and silver forging glycoside. According to the method, HPLC chromatographic detection conditions are reasonably optimized, the contents of six flavonoid components including rutin, isoquercitrin, kaempferol-3-O-beta-robinia bioside, kaempferol-3-O-rutinoside, kaempferol-3-O-beta-D-glucoside and silver forging glycoside can be simultaneously determined, and the method has the advantages of being easy to operate, stable, reliable, high in accuracy, good in reproducibility, high in sensitivity, high in accuracy, high in accuracy, high in accuracy, high in sensitivity, high in accuracy and the like. And the practicability is high.

The invention provides a folium ilicis cornutae extract as well as a preparation method and application thereof. The core component of the holly leaf extract comprises polyphenol compounds, the total polyphenol content is 10-500mg / g, and the polyphenol components comprise neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isoquercitrin, isochlorogenic acid B, isochlorogenic acid C and isochlorogenic acid A according to a ratio of (95-100): (300-450): (70-109): (120-256): (20-46): (21-156): (24-43): (27-61). The preparation method of the Chinese holly leaf extract comprises the whole processes of raw material pretreatment, multi-solvent synergistic extraction, multi-stage double-effect filtration and impurity removal, deep concentration and drying, parameters of all links are accurately controlled, the method is high in extraction rate, and the obtained Chinese holly leaf extract can effectively reduce the uric acid level and prevent and treat hyperuricemia.

The invention discloses an application of isoquercitrin in preparation of a medicine for improving insulin resistance and diabetes-related liver diseases, and belongs to the technical field of medicines. According to the application, a high-glucose and high-fat diet and streptozotocin combined method is adopted to successfully construct a type 2 diabetes mouse model; experiments find that the isoquercitrin can effectively relieve the symptoms of weight loss and water intake increase of T2DM mice, significantly reduce the fasting blood-glucose level and improve abnormal glucose tolerance; the isoquercitrin can reduce the liver index of a T2DM mouse, relieve fatty degeneration of the liver and repair a liver cell structure; by activating a PI3K / AKT signal channel, the isoquercitrin up-regulates the expression levels of PI3K, p-AKT, p-GSK3 and GLUT4 and down-regulates the expressions of GSK3 and PEPCK at the same time, so that the insulin resistance of T2DM mice is effectively improved, the fatty degeneration of the liver is improved, and the isoquercitrin has a protection or repair effect on liver injury.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to an establishment method of a mulberry paste fingerprint spectrum and the fingerprint spectrum. The establishment method of the mulberry paste fingerprint spectrum comprises the following steps: preparing a test solution by taking mulberry paste as a raw material, and respectively preparing reference solutions by taking chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, 5-hydroxymethylfurfural, rutin and isoquercitrin; respectively injecting the test solution and the reference solution into an ultra-high performance liquid chromatograph for determination, recording chromatograms, collecting the chromatograms of the test solutions of multiple batches of mulberry paste, introducing the chromatograms into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, generating a reference map, and calculating the similarity by taking the peak area and retention time as parameters. The method can be used for simultaneously detecting a plurality of components in the mulberry paste, and can be effectively used for evaluating the product quality stability of the mulberry paste.

The present invention discloses a method for screening effective substances of traditional Chinese medicine based on a 6-dimensional spider web model, belonging to the field of medical technology. Based on the results of UHPLC analysis, the present invention uses danshensu, chlorogenic acid, procyanidin B2, epicatechin, hyperoside, isoquercetin, rosmarinic acid, lithospermic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone I, tanshinone I, cryptotanshinone, tanshinone IIA, oleanolic acid, and ursolic acid as the "specific" candidate effective ingredients of the Danshen-Hawthorn medicinal pair. Based on the data of the candidate effective ingredients in six dimensions, namely, effectiveness dimension, compatibility environment dimension, transmission and traceability dimension, content measurability dimension, network pharmacology degree value dimension, and molecular docking target binding activity dimension, a "spider web" model is constructed to obtain the key effective substances of the Danshen-Hawthorn medicinal pair. The present invention provides theoretical support for revealing the compatibility theory of the Danshen-Hawthorn medicinal pair, formulating its quality evaluation standards, and clinical application of the Danshen-Hawthorn medicinal pair.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

The invention provides a construction method of the UPLC specific chromatogram of sword bean and preparation thereof, which comprises the following steps: A) dissolving a test raw material with a solvent, and extracting to obtain a to-be-detected solution; b) determining the liquid to be detected by adopting a high performance liquid chromatography to obtain the UPLC characteristic chromatogram of the sword bean and the preparation thereof; chromatographic conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 column; a mobile phase A is an acetonitrile solution, a mobile phase B is a 0.1% phosphoric acid aqueous solution, and gradient elution is carried out. According to the method, a high performance liquid chromatography is adopted, an acetonitrile-0. 1% phosphoric acid solution is selected as a mobile phase for gradient elution, quercetin-3-O-glucose-7-O-rhamnoside, myricetin-3-O-galactoside, lysimachin, rutin and isoquercitrin are taken as reference substances, and the UPLC characteristic spectrum method of the sword beans and the preparation thereof is established. And more scientific technical means are provided for controlling the medicinal quality of the sword beans and the preparation thereof.

The invention discloses application of a herba houttuyniae extract in preparation of a grouper iridovirus inhibitor. The herba houttuyniae extract is obtained by extracting a herba houttuyniae sample with water or an organic solvent. The herba houttuyniae extract prepared by the invention has a good inhibition effect on grouper iridovirus, does not generate drug resistance, has the characteristics of high efficiency, safety and no toxic or side effect, has a better antiviral effect compared with quercetin and isoquercitrin pure compounds, and has a wide application prospect in aquaculture.

The invention relates to application of enzyme modified isoquercitrin. The present invention provides a salivary secretion stimulator having a dosage form usable in the oral cavity or a dosage form usable by oral administration (dosage form for oral cavity, dosage form for oral administration). The salivary secretion stimulating agent may be prepared using an enzyme modified isoquercitrin or a combination of an enzyme modified isoquercitrin and a thickening polysaccharide. The invention also provides an additive (an additive for oral compositions), the composition is effective for preparing an oral composition (a food or beverage, pharmaceutical product for oral administration) and can impart at least one activity selected from the group consisting of salivary secretion stimulating activity, swallowing improving activity (swallowing assisting activity) and chewing improving activity (chewing assisting activity) to the oral composition. The additive may be prepared using an enzyme modified isoquercitrin in combination with a thickening polysaccharide.

The invention provides a method for detecting HPLC (high performance liquid chromatography) characteristic chromatograms of senecio scandens medicinal materials, decoction pieces, standard decoction and formula granules, which comprises the following steps: pre-treating raw materials of a test sample to obtain liquid to be detected; the pretreatment mode comprises solvent dissolution and extraction; measuring the liquid to be measured by adopting HPLC (High Performance Liquid Chromatography) to obtain HPLC characteristic chromatograms of the senecio scandens medicinal material, decoction pieces, standard decoction and formula granules; the preparation method comprises the following steps: preparing a reference substance solution, namely dissolving neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol by adopting a solvent; obtaining a reference substance solution of the reference substance; the HPLC conditions are as follows: a chromatographic column is a phenyl column; a mobile phase A is an acetonitrile solution, a mobile phase B is a 0.3% phosphoric acid aqueous solution, and gradient elution is carried out; according to the method, a high performance liquid chromatography method is adopted, and an acetonitrile-0. 3% phosphoric acid solution is selected as a mobile phase for gradient elution; the method comprises the following steps: taking neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol as reference substances; the HPLC characteristic chromatogram method of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules is established, and more scientific technical means are provided for controlling the medicinal quality of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules.

There are described compositions comprising an effective amount of a combination of two or more components, said components selected from acacetin, ACTI peptide, alpha-lipolic acid, alprostadil, anisomycin, apigenin, ascorbic acid, astragalus, berberine, β-lapachone, β-hydroxy-beta-methyl-butyrate, Bacopa monnieri, catechin, catechol, chamomile, chrysin, coumestrol, curcumin, dinitrophenol, dinoprost, ellagic acid, (−)-epigallocatechin gallate, green tea extract, fisetin, genistein, ginsenoside RE, glabridin, 18-α-glycyrrhetinic acid, 18-β-glycyrrhetinic acid, glycyrrhizin, hydroquinone, isoquercitrin (EMIQ), kaempferol, kuromanin, leucine, lithium, luteolin, luteolin, luteolinidin, melatonin, menadione, 1-methylnicotinamide (MNA), methyl salicylate, myricetin, nadide, niacin (vitamin B3), nicotinamide (NAM), nicotinamide mononucleotide (NMN), nicotinamide riboside (NR), nicotinic acid adenine dinucleotide (NaAD), nicotinic acid mononucleotide (NaMN), parsley (Petroselinium crispum), phenylephrine, pokeweed mitogen, 15-Δ prostaglandin J2, puromycin, quercetin, quinolinic acid, retinoic acid, trichostatin A, troxrutin, rutin, tryptophan, vitamin D3, withaferin A, wortmannin and zinc (including salts thereof).

The invention provides a feed additive for improving the superovulation effect of cows and a preparation method of the feed additive, and the feed additive comprises the following components in parts by mass: 4-6 parts of astragaloside, 4-6 parts of apigenin, 4-6 parts of kaempferol, 0.4-0.6 part of luteolin, 0.4-0.6 part of isoquercitrin, 0.2-0.4 part of formononetin and 0.2-0.4 part of quercetin. By using the feed additive, the ovulation number and the anti-oxidation and anti-inflammatory capacity of cows can be increased, meanwhile, the natural active ingredients in the feed additive do not need complex chemical synthesis steps, the breeding cost is reduced, animal health is improved, green and safe breeding is guaranteed, and a basis is provided for green development of modern animal husbandry.

The invention discloses an extraction method of a salvia miltiorrhiza-hawthorn medicine pair, and belongs to the technical field of traditional Chinese medicine extraction. According to the extraction method disclosed by the invention, the total content of tanshinol, chlorogenic acid, procyanidine B2, epicatechin, hyperoside, isoquercitrin, alkannic acid, rosmarinic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone and tanshinone IIA is taken as an extraction index, ethanol is taken as an extraction solvent, and the salvia miltiorrhiza-hawthorn medicine pair is subjected to reflux extraction. The process parameters in the extraction method are optimized by combining a single factor test with a Box-Behnken response surface method, and the salvia miltiorrhiza-hawthorn drug pair extract with the effective component content not lower than 8.0% is obtained. Based on a multi-index evaluation standard, a process with a better extraction effect is provided for development and application of the salvia miltiorrhiza-hawthorn medicine pair.

The invention provides a method for preparing high-purity isoquercitrin from polygonum viviparum, which comprises the following steps: sequentially carrying out water extraction impurity removal and alcohol extraction on a polygonum viviparum raw material to obtain an alcohol extract; decolorizing the alcohol extract, then adjusting the pH value to 9-11 to precipitate and remove tannin, and then carrying out vacuum concentration to obtain a general flavone crude product; performing acid hydrolysis on the crude product, performing separation and purification by adopting high-pressure preparative liquid chromatography, performing online monitoring by utilizing an ultraviolet detector, and obtaining a fraction rich in isoquercitrin by accurately controlling starting and ending points of fraction collection; and concentrating under reduced pressure, washing with water and drying to obtain the product. By optimizing the pretreatment and chromatographic separation conditions, the purity and yield of the product are synchronously improved, the HPLC (High Performance Liquid Chromatography) purity of the obtained isoquercitrin reaches 98% or above, and the method is low in process operation cost, environment-friendly and suitable for large-scale production.

The present invention discloses a method for multi-component determination of flavonoid components in young fruits of Abelmoschus esculentus, belonging to the technical field of traditional Chinese medicine detection. Using isoquercitrin as an internal reference substance, by calculating the relative correction factors of quercetin-3-O-gentiobioside, quercetin-3-O-xylose-(1-2)-glucose, and quercetin-3-O-(6”-O-malonyl)-glucoside, the contents of 4 flavonoid components are detected quickly and accurately, solving the problems that reference substances of the other three components except isoquercitrin are difficult to obtain and expensive, resulting in high detection costs; solving the problem that the solution state of quercetin-3-O-(6”-O-malonyl)-glucoside is unstable and easily converted into isoquercitrin, resulting in inaccurate content determination; solving the problem of changes in the content of flavonoid components caused by traditional drying methods, and being able to accurately reveal the true situation of flavonoid components in young fruits of Abelmoschus esculentus.

The invention provides an application of isoquercitrin in preparation of a medicine for treating systemic lupus erythematosus. The invention further provides a medicine for treating systemic lupus erythematosus. The medicine contains an effective therapeutic dose of isoquercitrin. The invention provides a novel method for treating systemic lupus erythematosus.

The amino acid sequence of the novel thermophilic alpha-L-rhamnosidase is shown as SEQ ID No.1, and the novel thermophilic alpha-L-rhamnosidase is derived from a metagenome of a hot spring in Yuanjiang County in Yunnan province. The thermophilic alpha-L-rhamnosidase YJ-Rha72 provided by the invention can hydrolyze flavonoid substances such as rutin, naringin, hesperidin, neohesperidin and the like and p-nitrophenyl-alpha-L-rhamnopyranoside (pNPR), the rutin hydrolysis activity is the highest, and the thermophilic alpha-L-rhamnosidase YJ-Rha72 has the hydrolytic activity of alpha-1, alpha-1, 2 and alpha-1, 6 glucosidic bonds. The optimum reaction temperature of the enzyme is 75 DEG C, and the activity of the enzyme at 50-85 DEG C exceeds 34.9%; the optimum reaction pH is 6.0, and the activity exceeds 74% in the pH range of 5-7.5. The enzyme can completely hydrolyze 1 mM of rutin within 20 minutes, and the conversion rate is 100%. The alpha-L-rhamnosidase YJ-Rha72 has important application potential and value, for example, the alpha-L-rhamnosidase YJ-Rha72 is used for preparing important bioactive substances such as isoquercitrin and Prunin through biotransformation of rhamnoside substances, debitterizing citrus juice, enhancing aroma of grape wine and the like.

The present invention relates to the technical field of detection of traditional Chinese medicine components, and particularly to a quality detection method for Sichuan parasitic herbs and their preparations, including: detecting the test solution and the reference solution by high performance liquid chromatography; the test solution contains Sichuan parasitic herbs; the reference solutions are gallic acid solution, protocatechuic acid solution, catechin solution, isoquercitrin solution and quercitrin solution respectively; the conditions for high performance liquid chromatography detection include: the mobile phase includes mobile phase A and mobile phase B, acetonitrile is used as mobile phase A, and glacial acetic acid solution is used as mobile phase B for gradient elution; the flow rate of the mobile phase is 0.8-1.2 mL / min. By establishing a new characteristic chromatogram method to control its quality, it can solve the problem that it is difficult to comprehensively evaluate and control the process from Sichuan parasitic herbs to the finished Sichuan parasitic formula granules in the prior art, and the quality detection of Sichuan parasitic herbs in production is comprehensive and reliable, and the detection time is relatively short.

The present invention relates to a method for preparing isoquercetin that inhibits xanthine oxidase activity, characterized by comprising the following steps: (1) using dried tangerine peel as a raw material, extracting it with an organic liquid, combining the extracts, and draining the solvent using a rotary evaporator to obtain an organic layer extract AE; (2) separating and purifying the organic layer extract AE through a column to obtain component AE-3; (3) separating and purifying component AE-3 again to obtain component AE-3-7; and identifying component AE-3-7 as containing isoquercetin by nuclear magnetic resonance; and (4) conducting an enzyme activity inhibition experiment. The preparation process of the present invention is simple and easy to operate; the obtained isoquercetin is extracted, separated and purified from dried tangerine peel, and is found to have the ability to inhibit xanthine oxidase activity, providing a new source for the prevention and treatment of hyperuricemia, and having great significance for the research on the effective ingredients of natural products of Chinese medicinal materials with medicinal and edible properties.

The invention provides hydrogel carrying isoquercitrin, a preparation method of the hydrogel and application of the hydrogel in myocardial oxidative damage resistance, and belongs to the technical field of biological medicine. The invention provides a hydrogel loaded with isoquercitrin, and particularly provides a preparation method of the hydrogel. According to the hydrogel, the pharmacological activity of the isoquercitrin is reserved, the problem that the water solubility of the isoquercitrin is poor is remarkably solved, the biocompatibility and the solubility of the isoquercitrin are enhanced, a better treatment effect is shown on the cardiomyopathy induced by oxidative stress, and the hydrogel can be applied to the treatment of the oxidative stress induced cardiomyopathy. And the application of the isoquercitrin in the treatment direction of cardiomyopathy-related diseases is expanded.

A method for preparing an anti-tumor traditional Chinese medicine compound formulation containing isoquercitrin. The specific preparation steps of the anti-tumor traditional Chinese medicine compound formulation containing isoquercitrin are as follows: 8-10 g of Taxus chinensis, 6-12 g of Paris polyphylla, 15-17 g of Solanum nigrum, 3-7 g of Taraxacum officinale, 5-10 g of Angelica sinensis, 12-14 g of Poria cocos, 13-15g of Astragalus membranaceus, 8-10g of Panax ginseng,13-15g of Ganoderma lucidum, 8-10g of Scutellaria barbata, 13-15g of Sophora flavescens, 14-16g of Rabdosia rubescens, 8-12g of Panax notoginseng, 15-17g of Scleromitrion diffusum, 16-17g of Lithospermum erythrorhizon, 8-12g of Morus alba, and 13-14g of Sophora japonica. The raw material formula of the present application incorporates Morus alba and Sophora japonica, and the Morus alba and Sophora japonica are rich in isoquercitrin and have antioxidant, anti-inflammatory and potential antitumor effects, thus significantly enhancing the efficacy of the traditional Chinese medicine compound formulation. Moreover, the preparation process of the formulation is simple. After the preparation of the formulation is completed, the concentration of the isoquercitrin content can be quantitatively determined by using high-performance liquid chromatography technology, and an acute toxicity test and a long-term toxicity test are performed to ensure the safety of the formulation.

The present invention provides a saliva secretion stimulating agent having a dosage form usable in the oral cavity or a dosage form usable by oral administration (dosage form for the oral cavity, dosage form for oral administration). The saliva secretion stimulating agent can be prepared using enzyme-modified isoquercitrin or a combination of enzyme-modified isoquercitrin and a thickening polysaccharide. The present invention also provides an additive (additive for oral composition) that is effective for preparing an oral composition (food or beverage, pharmaceutical product for oral administration) and can impart at least one activity selected from the group consisting of saliva secretion stimulating activity, swallowing improvement activity (swallowing aid activity), and mastication improvement activity (mastication aid activity) to the oral composition. The additive can be prepared using a combination of enzyme-modified isoquercitrin and a thickening polysaccharide.

The invention discloses a method for detecting the content of isoquercitrin in a chingma abutilon seed sample and application of the method. A test solution and a reference solution are prepared respectively and injected into an ultra-high performance liquid chromatograph, and specific chromatograms of the reference solution and the test solution are compared; and calculating the content of the isoquercitrin in the chingma abutilon seed sample by utilizing a regression equation of the sample size and the peak area of the isoquercitrin. The method disclosed by the invention is good in specificity and durability, and a new analysis means can be provided for quality detection of a chingma abutilon seed sample; the specific component of isoquercitrin is taken as an index, so that the method is more reasonable and accurate; the method is applicable to detection of chingma abutilon seed medicinal materials, standard decoction, formula granules and other products containing chingma abutilon seeds. The method is simple to operate, good in reproducibility and stability, reliable in recovery rate, low in detection cost, high in detection efficiency and small in environmental pollution.

This invention discloses a method for determining the quality of *Hemiberlesia lingua* stems and leaves. The method includes fingerprint spectroscopy establishment and similarity analysis, content determination, cluster analysis, principal component analysis, and partial least squares discriminant analysis. The method of this invention involves UPLC fingerprint analysis and similarity analysis of 15 batches of *Hemiberlesia lingua* stems and leaves from different origins. A total of 28 common peaks were found, and 7 of these common peaks were identified using reference standards: peak 4 (neochlorogenic acid), peak 8 (chlorogenic acid), peak 9 (caffeic acid), peak 11 (cryptochlorogenic acid), peak 22 (isoquercetin), peak 24 (astragalin), and peak 28 (isochlorogenic acid C). This invention's method is highly sensitive, specific, stable, and feasible, providing a reference for the quality evaluation and control of *Hemiberlesia lingua* stems and leaves.

The present application relates to the application of isoquercitrin in the preparation of a drug for treating post-traumatic stress disorder, and belongs to the technical field of natural chemical medicine development. The present application systematically clarifies the whole-chain mechanism of isoquercitrin in treating PTSD through the "GABA A R-PINK1 / Parkin-mitophagy" pathway by means of a variety of technical means such as molecular docking, microthermal swimming, patch clamp, proteomics, cell and animal models. Compared with the clinically commonly used drugs for treating post-traumatic stress disorder, isoquercitrin, as a natural flavonoid compound, can improve the symptoms of PTSD while not damaging the learning and memory function, and has better safety. Moreover, isoquercitrin is widely sourced and has a mature preparation process. The present application provides a theoretical basis and experimental support for the application of isoquercitrin in the development of drugs for treating post-traumatic stress disorder, and has important clinical application value and industrialization prospects.

The present application relates to the technical field of traditional Chinese medicine analysis, and particularly relates to a mulberry fruit paste fingerprint spectrum establishment method and fingerprint spectrum. The mulberry fruit paste fingerprint spectrum establishment method comprises the following steps: taking mulberry fruit paste as raw material to prepare a test sample solution, taking chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, 5-hydroxymethyl furfural, rutin and isoquercitrin to prepare reference sample solutions; injecting the test sample solution and the reference sample solutions into an ultra-high performance liquid chromatograph for determination, recording chromatograms, collecting chromatograms of test sample solutions of multiple batches of mulberry fruit paste, importing a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, generating a control spectrum, and taking peak area and retention time as parameters to calculate similarity. The method can simultaneously detect multiple components in mulberry fruit paste, and can be effectively used to evaluate product quality stability of mulberry fruit paste.

The invention discloses a traditional Chinese medicine efficacy substance screening method based on a 6-dimensional cobweb model, and belongs to the technical field of medicine. Based on a UHPLC (Ultra High Performance Liquid Chromatography) analysis result, tanshinol, chlorogenic acid, procyanidine B2, epicatechin, hyperoside, isoquercitrin, rosmarinic acid, alkannic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone I, tanshinone I, cryptotanshinone, tanshinone IIA, oleanolic acid and ursolic acid are used as specific candidate functional components of the salvia miltiorrhiza-hawthorn medicine pair; and constructing a cobweb model based on data of the candidate functional components in six dimensions, namely an effectiveness dimension, a compatibility environment dimension, a transmission and traceability dimension, a content testability dimension, a network pharmacology value dimension and a molecular docking target binding activity dimension, so as to obtain the key medicinal effect substances of the salvia miltiorrhiza-hawthorn drug pair. The invention provides theoretical support for revealing the compatibility theory of the salvia miltiorrhiza-hawthorn medicine pair, formulating the quality evaluation standard and clinical application of the salvia miltiorrhiza-hawthorn medicine pair.