49 results about "Isoquercitroside" patented technology

The invention discloses a new application of isoquercitrin in preparation of a medicine for treating renal interstitial fibrosis, and finds and verifies that the isoquercitrin can be used for effectively treating the renal interstitial fibrosis through experiments for the first time, and the isoquercitrin is a natural flavonoid compound. The isoquercitrin has the advantages of low toxic and side effects, high safety, high effectiveness and the like, in addition, the isoquercitrin is rich in raw material resource and has good social and economic benefits, a brand new thought and strategy are provided for research and development of renal interstitial fibrosis treatment medicines, and the application prospect in the technical field of renal interstitial fibrosis treatment is wide.

The invention discloses application of a glucosyl modified quercetin derivative and a composition containing the glucosyl modified quercetin derivative. Specifically, the invention discloses an application of glucosyl modified quercetin as an anti-skin photoaging agent. According to the application disclosed by the invention, glucosyl modified quercetin, especially glucosyl modified isoquercitrin, has a remarkable effect in the aspect of resisting skin photoaging; cosmetics prepared from glucosyl modified quercetin have good anti-aging ability, and can significantly improve percutaneous water loss, skin elasticity and skin roughness.

The application discloses a kind of cydia pomella larva's food lure composition and application thereof.It includes the following weight parts of component: isoquercitrin 0.02~0.07 parts, phlorizin 0.6~0.9 parts, cyanidin-3-O-galactoside 0.01~0.02 parts, delphinidin-3-O-galactose 0.05~0.12 parts.The food lure component provided in the application has low dosage and low price, can improve the behavior regulation effect of lure core on larvae, reduce the production cost, and is suitable for popularization and promotion.

To provide a composition that has the effect of improving unidentified complaint, depression, decreased concentration, decreased motivation to socialize and engage in daily activities, autonomic nervous system disorders, pain, control, and poor quality of sleep, associated with menstruation.SOLUTION: Provided is a composition for improving premenstrual depression, containing as an active ingredient an Apocynum venetum leaf extract containing 4 to 10 wt.% in total of the flavonoid compounds hyperoside and isoquercitrin, and in which the daily intake of the Apocynum venetum leaf extract is in the range of 40 mg to 100 mg or less (limited to healthy adult women who are undergoing treatment for premenstrual syndrome and premenstrual dysphoric disorder or have no history of such syndrome).SELECTED DRAWING: None

The invention belongs to the technical field of chemical drugs, and particularly relates to application of a polyphenol composition containing gallic acid to preparation of drugs for treating inflammatory bowel diseases and the drugs. The polyphenol composition contains caffeic acid, ferulic acid, gallic acid, hesperidin, isoquercitrin, isorhamnetin, kaempferol, protocatechuic acid and quercetin, and has an obvious anti-inflammatory effect on the inflammatory bowel disease; when the composition is used as a medicine for treating the inflammatory bowel disease, the treatment cost can be reduced, and the composition is effective in treating the inflammatory bowel disease and small in toxic and side effects after being taken for a long time.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a method for determining the content of five flavonoid components in a hypericum perforatum medicinal material. The content determination method comprises the following steps: preparing rutin, hyperoside, isoquercitrin, quercitrin and quercetin into a mixed reference solution; extracting a hypericum perforatum medicinal material by using an extraction solvent to prepare a test solution; the mixed reference substance solutions and the test solution with different volumes are subjected to high performance liquid chromatography respectively, a mobile phase A is acetonitrile, a mobile phase B is a phosphoric acid aqueous solution with the volume concentration of 0.08-0.12%, and elution gradient is adopted. The content determination method has the advantages of high sensitivity, simplicity and convenience in operation, low cost and high detection speed.

The invention belongs to the technical field of traditional Chinese medicine analysis, and particularly relates to a method for detecting various flavonoid components in cottonrose hibiscus leaf medicinal materials. The method comprises the following steps: detecting a test sample by adopting HPLC (High Performance Liquid Chromatography) to obtain a high performance liquid chromatography detection result of flavonoid components in the test sample; the test sample is a cotton rose hibiscus leaf medicinal material extraction solution, and the flavonoid components comprise at least four of rutin, isoquercitrin, kaempferol-3-O-beta-robinia bioside, kaempferol-3-O-rutinoside, kaempferol-3-O-beta-D-glucoside and silver forging glycoside. According to the method, HPLC chromatographic detection conditions are reasonably optimized, the contents of six flavonoid components including rutin, isoquercitrin, kaempferol-3-O-beta-robinia bioside, kaempferol-3-O-rutinoside, kaempferol-3-O-beta-D-glucoside and silver forging glycoside can be simultaneously determined, and the method has the advantages of being easy to operate, stable, reliable, high in accuracy, good in reproducibility, high in sensitivity, high in accuracy, high in accuracy, high in accuracy, high in sensitivity, high in accuracy and the like. And the practicability is high.

The invention provides a folium ilicis cornutae extract as well as a preparation method and application thereof. The core component of the holly leaf extract comprises polyphenol compounds, the total polyphenol content is 10-500mg / g, and the polyphenol components comprise neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isoquercitrin, isochlorogenic acid B, isochlorogenic acid C and isochlorogenic acid A according to a ratio of (95-100): (300-450): (70-109): (120-256): (20-46): (21-156): (24-43): (27-61). The preparation method of the Chinese holly leaf extract comprises the whole processes of raw material pretreatment, multi-solvent synergistic extraction, multi-stage double-effect filtration and impurity removal, deep concentration and drying, parameters of all links are accurately controlled, the method is high in extraction rate, and the obtained Chinese holly leaf extract can effectively reduce the uric acid level and prevent and treat hyperuricemia.

The invention relates to the technical field of cosmetics, in particular to anti-hair-loss and oil-control shampoo and a preparation method thereof. The hair loss prevention and oil control shampoo is prepared from the following components in parts by weight: 11 to 40 parts of a surfactant, 2 to 20 parts of a platycladus orientalis extract, 0.1 to 0.8 part of a cationic conditioner, 0.05 to 0.2 part of a chelating agent, 0.3 to 1.3 parts of a rheology modifier and 50 to 75 parts of water, the platycladus orientalis extract is prepared from isoquercitrin, afzelin, amentoflavone, pinus koraiensis lactone and dextro-quinic acid in the mass ratio of the isoquercitrin to the afzelin to the amentoflavone to the pinus koraiensis lactone to the dextro-quinic acid being 1 to (2 to 2.7) to (3.5 to 5) to (2 to 2.6) to (650 to 890). Flocculation is generated through the synergistic effect of the platycladus orientalis extract and the cationic conditioner, the composition can be better attached to the scalp, the retention time is prolonged, and the anti-hair-loss and oil-control effects are remarkably improved.

To provide a composition for improving liver health.SOLUTION: In one aspect, the composition of the present invention includes a complex component of Gallocatechin Gallate and Isoquercitrin, which effectively reduces oxidative stress and chronic inflammatory reaction that occur during the accumulation of lipids in liver tissue, thereby improving liver health and enabling the prevention, improvement, or treatment of various liver diseases such as hepatitis, cirrhosis, and liver cancer.SELECTED DRAWING: Figure 1

The invention discloses an application of isoquercitrin in preparation of a medicine for improving insulin resistance and diabetes-related liver diseases, and belongs to the technical field of medicines. According to the application, a high-glucose and high-fat diet and streptozotocin combined method is adopted to successfully construct a type 2 diabetes mouse model; experiments find that the isoquercitrin can effectively relieve the symptoms of weight loss and water intake increase of T2DM mice, significantly reduce the fasting blood-glucose level and improve abnormal glucose tolerance; the isoquercitrin can reduce the liver index of a T2DM mouse, relieve fatty degeneration of the liver and repair a liver cell structure; by activating a PI3K / AKT signal channel, the isoquercitrin up-regulates the expression levels of PI3K, p-AKT, p-GSK3 and GLUT4 and down-regulates the expressions of GSK3 and PEPCK at the same time, so that the insulin resistance of T2DM mice is effectively improved, the fatty degeneration of the liver is improved, and the isoquercitrin has a protection or repair effect on liver injury.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to an establishment method of a mulberry paste fingerprint spectrum and the fingerprint spectrum. The establishment method of the mulberry paste fingerprint spectrum comprises the following steps: preparing a test solution by taking mulberry paste as a raw material, and respectively preparing reference solutions by taking chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, 5-hydroxymethylfurfural, rutin and isoquercitrin; respectively injecting the test solution and the reference solution into an ultra-high performance liquid chromatograph for determination, recording chromatograms, collecting the chromatograms of the test solutions of multiple batches of mulberry paste, introducing the chromatograms into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, generating a reference map, and calculating the similarity by taking the peak area and retention time as parameters. The method can be used for simultaneously detecting a plurality of components in the mulberry paste, and can be effectively used for evaluating the product quality stability of the mulberry paste.

The present invention discloses a method for screening effective substances of traditional Chinese medicine based on a 6-dimensional spider web model, belonging to the field of medical technology. Based on the results of UHPLC analysis, the present invention uses danshensu, chlorogenic acid, procyanidin B2, epicatechin, hyperoside, isoquercetin, rosmarinic acid, lithospermic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone I, tanshinone I, cryptotanshinone, tanshinone IIA, oleanolic acid, and ursolic acid as the "specific" candidate effective ingredients of the Danshen-Hawthorn medicinal pair. Based on the data of the candidate effective ingredients in six dimensions, namely, effectiveness dimension, compatibility environment dimension, transmission and traceability dimension, content measurability dimension, network pharmacology degree value dimension, and molecular docking target binding activity dimension, a "spider web" model is constructed to obtain the key effective substances of the Danshen-Hawthorn medicinal pair. The present invention provides theoretical support for revealing the compatibility theory of the Danshen-Hawthorn medicinal pair, formulating its quality evaluation standards, and clinical application of the Danshen-Hawthorn medicinal pair.

In the present specification, disclosed is a method for preparing a gallocatechin gallate (Gallocatable Gallate), which comprises the steps of: preparing a gallocatechin gallate (Gallocatable Gallate; the present invention relates to uses of a composition for enhancing liver health or for auxiliary protection against chemical liver injury, in particular to uses of a composition for enhancing liver health or for auxiliary protection against chemical liver injury, such as GCG, isoquercitrin, IQC, and to the preparation of a composition for enhancing liver health or for auxiliary protection against chemical liver injury. In one aspect, the composition of the present invention can include a composite component of gallocatechin gallate and isoquercitrin to effectively reduce oxidative stress and chronic inflammatory response occurring in the process of lipid accumulation in liver tissue to improve liver health, and is applied to prevention, improvement or treatment of various liver diseases such as hepatitis, cirrhosis, liver cancer and the like.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

To provide a new use of Hibiscus 'Titanbicus'.SOLUTION: An angiotensin-converting enzyme inhibitor contain Hibiscus 'Titanbicus' containing hyperoside and isoquercitrin at a ratio of (1:3) to (2:1), for example, a flower (including bud) or its extract of Hibiscus 'Titanbicus' peach white (kind registration by the Ministry of Agriculture, Forestry and Fisheries No. 21813), and contain a Hibiscus 'Titanbicus' perennial plant, as the flower (including bud) or its extract of Hibiscus 'Titanbicus', wherein a total amount of the hyperoside and the isoquercitrin contained in the Hibiscus 'Titanbicus' perennial plant is 1.0 mass% or more.SELECTED DRAWING: Figure 1

The invention provides a construction method of the UPLC specific chromatogram of sword bean and preparation thereof, which comprises the following steps: A) dissolving a test raw material with a solvent, and extracting to obtain a to-be-detected solution; b) determining the liquid to be detected by adopting a high performance liquid chromatography to obtain the UPLC characteristic chromatogram of the sword bean and the preparation thereof; chromatographic conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 column; a mobile phase A is an acetonitrile solution, a mobile phase B is a 0.1% phosphoric acid aqueous solution, and gradient elution is carried out. According to the method, a high performance liquid chromatography is adopted, an acetonitrile-0. 1% phosphoric acid solution is selected as a mobile phase for gradient elution, quercetin-3-O-glucose-7-O-rhamnoside, myricetin-3-O-galactoside, lysimachin, rutin and isoquercitrin are taken as reference substances, and the UPLC characteristic spectrum method of the sword beans and the preparation thereof is established. And more scientific technical means are provided for controlling the medicinal quality of the sword beans and the preparation thereof.

The invention discloses application of a herba houttuyniae extract in preparation of a grouper iridovirus inhibitor. The herba houttuyniae extract is obtained by extracting a herba houttuyniae sample with water or an organic solvent. The herba houttuyniae extract prepared by the invention has a good inhibition effect on grouper iridovirus, does not generate drug resistance, has the characteristics of high efficiency, safety and no toxic or side effect, has a better antiviral effect compared with quercetin and isoquercitrin pure compounds, and has a wide application prospect in aquaculture.

The invention relates to application of enzyme modified isoquercitrin. The present invention provides a salivary secretion stimulator having a dosage form usable in the oral cavity or a dosage form usable by oral administration (dosage form for oral cavity, dosage form for oral administration). The salivary secretion stimulating agent may be prepared using an enzyme modified isoquercitrin or a combination of an enzyme modified isoquercitrin and a thickening polysaccharide. The invention also provides an additive (an additive for oral compositions), the composition is effective for preparing an oral composition (a food or beverage, pharmaceutical product for oral administration) and can impart at least one activity selected from the group consisting of salivary secretion stimulating activity, swallowing improving activity (swallowing assisting activity) and chewing improving activity (chewing assisting activity) to the oral composition. The additive may be prepared using an enzyme modified isoquercitrin in combination with a thickening polysaccharide.

The invention provides a method for detecting HPLC (high performance liquid chromatography) characteristic chromatograms of senecio scandens medicinal materials, decoction pieces, standard decoction and formula granules, which comprises the following steps: pre-treating raw materials of a test sample to obtain liquid to be detected; the pretreatment mode comprises solvent dissolution and extraction; measuring the liquid to be measured by adopting HPLC (High Performance Liquid Chromatography) to obtain HPLC characteristic chromatograms of the senecio scandens medicinal material, decoction pieces, standard decoction and formula granules; the preparation method comprises the following steps: preparing a reference substance solution, namely dissolving neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol by adopting a solvent; obtaining a reference substance solution of the reference substance; the HPLC conditions are as follows: a chromatographic column is a phenyl column; a mobile phase A is an acetonitrile solution, a mobile phase B is a 0.3% phosphoric acid aqueous solution, and gradient elution is carried out; according to the method, a high performance liquid chromatography method is adopted, and an acetonitrile-0. 3% phosphoric acid solution is selected as a mobile phase for gradient elution; the method comprises the following steps: taking neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol as reference substances; the HPLC characteristic chromatogram method of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules is established, and more scientific technical means are provided for controlling the medicinal quality of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules.

There are described compositions comprising an effective amount of a combination of two or more components, said components selected from acacetin, ACTI peptide, alpha-lipolic acid, alprostadil, anisomycin, apigenin, ascorbic acid, astragalus, berberine, β-lapachone, β-hydroxy-beta-methyl-butyrate, Bacopa monnieri, catechin, catechol, chamomile, chrysin, coumestrol, curcumin, dinitrophenol, dinoprost, ellagic acid, (−)-epigallocatechin gallate, green tea extract, fisetin, genistein, ginsenoside RE, glabridin, 18-α-glycyrrhetinic acid, 18-β-glycyrrhetinic acid, glycyrrhizin, hydroquinone, isoquercitrin (EMIQ), kaempferol, kuromanin, leucine, lithium, luteolin, luteolin, luteolinidin, melatonin, menadione, 1-methylnicotinamide (MNA), methyl salicylate, myricetin, nadide, niacin (vitamin B3), nicotinamide (NAM), nicotinamide mononucleotide (NMN), nicotinamide riboside (NR), nicotinic acid adenine dinucleotide (NaAD), nicotinic acid mononucleotide (NaMN), parsley (Petroselinium crispum), phenylephrine, pokeweed mitogen, 15-Δ prostaglandin J2, puromycin, quercetin, quinolinic acid, retinoic acid, trichostatin A, troxrutin, rutin, tryptophan, vitamin D3, withaferin A, wortmannin and zinc (including salts thereof).

The invention provides a feed additive for improving the superovulation effect of cows and a preparation method of the feed additive, and the feed additive comprises the following components in parts by mass: 4-6 parts of astragaloside, 4-6 parts of apigenin, 4-6 parts of kaempferol, 0.4-0.6 part of luteolin, 0.4-0.6 part of isoquercitrin, 0.2-0.4 part of formononetin and 0.2-0.4 part of quercetin. By using the feed additive, the ovulation number and the anti-oxidation and anti-inflammatory capacity of cows can be increased, meanwhile, the natural active ingredients in the feed additive do not need complex chemical synthesis steps, the breeding cost is reduced, animal health is improved, green and safe breeding is guaranteed, and a basis is provided for green development of modern animal husbandry.

The invention discloses an extraction method of a salvia miltiorrhiza-hawthorn medicine pair, and belongs to the technical field of traditional Chinese medicine extraction. According to the extraction method disclosed by the invention, the total content of tanshinol, chlorogenic acid, procyanidine B2, epicatechin, hyperoside, isoquercitrin, alkannic acid, rosmarinic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone and tanshinone IIA is taken as an extraction index, ethanol is taken as an extraction solvent, and the salvia miltiorrhiza-hawthorn medicine pair is subjected to reflux extraction. The process parameters in the extraction method are optimized by combining a single factor test with a Box-Behnken response surface method, and the salvia miltiorrhiza-hawthorn drug pair extract with the effective component content not lower than 8.0% is obtained. Based on a multi-index evaluation standard, a process with a better extraction effect is provided for development and application of the salvia miltiorrhiza-hawthorn medicine pair.

The invention provides a method for preparing high-purity isoquercitrin from polygonum viviparum, which comprises the following steps: sequentially carrying out water extraction impurity removal and alcohol extraction on a polygonum viviparum raw material to obtain an alcohol extract; decolorizing the alcohol extract, then adjusting the pH value to 9-11 to precipitate and remove tannin, and then carrying out vacuum concentration to obtain a general flavone crude product; performing acid hydrolysis on the crude product, performing separation and purification by adopting high-pressure preparative liquid chromatography, performing online monitoring by utilizing an ultraviolet detector, and obtaining a fraction rich in isoquercitrin by accurately controlling starting and ending points of fraction collection; and concentrating under reduced pressure, washing with water and drying to obtain the product. By optimizing the pretreatment and chromatographic separation conditions, the purity and yield of the product are synchronously improved, the HPLC (High Performance Liquid Chromatography) purity of the obtained isoquercitrin reaches 98% or above, and the method is low in process operation cost, environment-friendly and suitable for large-scale production.

To provide a new use of Hibiscus 'Titanbicus'.SOLUTION: An anti-anxiety agent and an angiotensin-converting enzyme inhibitor contain Hibiscus 'Titanbicus' containing hyperoside and isoquercitrin at a ratio of (1:3) to (2:1), for example, a flower (including bud) or its extract of Hibiscus 'Titanbicus' peach white (kind registration by the Ministry of Agriculture, Forestry and Fisheries No. 21813), and contain a Hibiscus 'Titanbicus' perennial plant, as the flower (including bud) or its extract of Hibiscus 'Titanbicus', wherein a total amount of the hyperoside and the isoquercitrin contained in the Hibiscus 'Titanbicus' perennial plant is 1.0 mass% or more.SELECTED DRAWING: Figure 1

The present invention discloses a method for multi-component determination of flavonoid components in young fruits of Abelmoschus esculentus, belonging to the technical field of traditional Chinese medicine detection. Using isoquercitrin as an internal reference substance, by calculating the relative correction factors of quercetin-3-O-gentiobioside, quercetin-3-O-xylose-(1-2)-glucose, and quercetin-3-O-(6”-O-malonyl)-glucoside, the contents of 4 flavonoid components are detected quickly and accurately, solving the problems that reference substances of the other three components except isoquercitrin are difficult to obtain and expensive, resulting in high detection costs; solving the problem that the solution state of quercetin-3-O-(6”-O-malonyl)-glucoside is unstable and easily converted into isoquercitrin, resulting in inaccurate content determination; solving the problem of changes in the content of flavonoid components caused by traditional drying methods, and being able to accurately reveal the true situation of flavonoid components in young fruits of Abelmoschus esculentus.

The invention provides an application of isoquercitrin in preparation of a medicine for treating systemic lupus erythematosus. The invention further provides a medicine for treating systemic lupus erythematosus. The medicine contains an effective therapeutic dose of isoquercitrin. The invention provides a novel method for treating systemic lupus erythematosus.

The amino acid sequence of the novel thermophilic alpha-L-rhamnosidase is shown as SEQ ID No.1, and the novel thermophilic alpha-L-rhamnosidase is derived from a metagenome of a hot spring in Yuanjiang County in Yunnan province. The thermophilic alpha-L-rhamnosidase YJ-Rha72 provided by the invention can hydrolyze flavonoid substances such as rutin, naringin, hesperidin, neohesperidin and the like and p-nitrophenyl-alpha-L-rhamnopyranoside (pNPR), the rutin hydrolysis activity is the highest, and the thermophilic alpha-L-rhamnosidase YJ-Rha72 has the hydrolytic activity of alpha-1, alpha-1, 2 and alpha-1, 6 glucosidic bonds. The optimum reaction temperature of the enzyme is 75 DEG C, and the activity of the enzyme at 50-85 DEG C exceeds 34.9%; the optimum reaction pH is 6.0, and the activity exceeds 74% in the pH range of 5-7.5. The enzyme can completely hydrolyze 1 mM of rutin within 20 minutes, and the conversion rate is 100%. The alpha-L-rhamnosidase YJ-Rha72 has important application potential and value, for example, the alpha-L-rhamnosidase YJ-Rha72 is used for preparing important bioactive substances such as isoquercitrin and Prunin through biotransformation of rhamnoside substances, debitterizing citrus juice, enhancing aroma of grape wine and the like.

The present invention relates to the technical field of detection of traditional Chinese medicine components, and particularly to a quality detection method for Sichuan parasitic herbs and their preparations, including: detecting the test solution and the reference solution by high performance liquid chromatography; the test solution contains Sichuan parasitic herbs; the reference solutions are gallic acid solution, protocatechuic acid solution, catechin solution, isoquercitrin solution and quercitrin solution respectively; the conditions for high performance liquid chromatography detection include: the mobile phase includes mobile phase A and mobile phase B, acetonitrile is used as mobile phase A, and glacial acetic acid solution is used as mobile phase B for gradient elution; the flow rate of the mobile phase is 0.8-1.2 mL / min. By establishing a new characteristic chromatogram method to control its quality, it can solve the problem that it is difficult to comprehensively evaluate and control the process from Sichuan parasitic herbs to the finished Sichuan parasitic formula granules in the prior art, and the quality detection of Sichuan parasitic herbs in production is comprehensive and reliable, and the detection time is relatively short.

The present invention relates to a method for preparing isoquercetin that inhibits xanthine oxidase activity, characterized by comprising the following steps: (1) using dried tangerine peel as a raw material, extracting it with an organic liquid, combining the extracts, and draining the solvent using a rotary evaporator to obtain an organic layer extract AE; (2) separating and purifying the organic layer extract AE through a column to obtain component AE-3; (3) separating and purifying component AE-3 again to obtain component AE-3-7; and identifying component AE-3-7 as containing isoquercetin by nuclear magnetic resonance; and (4) conducting an enzyme activity inhibition experiment. The preparation process of the present invention is simple and easy to operate; the obtained isoquercetin is extracted, separated and purified from dried tangerine peel, and is found to have the ability to inhibit xanthine oxidase activity, providing a new source for the prevention and treatment of hyperuricemia, and having great significance for the research on the effective ingredients of natural products of Chinese medicinal materials with medicinal and edible properties.