14 results about "Isoquercitroside" patented technology

The invention discloses an application of isoquercitrin in preparation of a medicine for improving insulin resistance and diabetes-related liver diseases, and belongs to the technical field of medicines. According to the application, a high-glucose and high-fat diet and streptozotocin combined method is adopted to successfully construct a type 2 diabetes mouse model; experiments find that the isoquercitrin can effectively relieve the symptoms of weight loss and water intake increase of T2DM mice, significantly reduce the fasting blood-glucose level and improve abnormal glucose tolerance; the isoquercitrin can reduce the liver index of a T2DM mouse, relieve fatty degeneration of the liver and repair a liver cell structure; by activating a PI3K / AKT signal channel, the isoquercitrin up-regulates the expression levels of PI3K, p-AKT, p-GSK3 and GLUT4 and down-regulates the expressions of GSK3 and PEPCK at the same time, so that the insulin resistance of T2DM mice is effectively improved, the fatty degeneration of the liver is improved, and the isoquercitrin has a protection or repair effect on liver injury.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

The invention discloses application of a herba houttuyniae extract in preparation of a grouper iridovirus inhibitor. The herba houttuyniae extract is obtained by extracting a herba houttuyniae sample with water or an organic solvent. The herba houttuyniae extract prepared by the invention has a good inhibition effect on grouper iridovirus, does not generate drug resistance, has the characteristics of high efficiency, safety and no toxic or side effect, has a better antiviral effect compared with quercetin and isoquercitrin pure compounds, and has a wide application prospect in aquaculture.

The invention relates to application of enzyme modified isoquercitrin. The present invention provides a salivary secretion stimulator having a dosage form usable in the oral cavity or a dosage form usable by oral administration (dosage form for oral cavity, dosage form for oral administration). The salivary secretion stimulating agent may be prepared using an enzyme modified isoquercitrin or a combination of an enzyme modified isoquercitrin and a thickening polysaccharide. The invention also provides an additive (an additive for oral compositions), the composition is effective for preparing an oral composition (a food or beverage, pharmaceutical product for oral administration) and can impart at least one activity selected from the group consisting of salivary secretion stimulating activity, swallowing improving activity (swallowing assisting activity) and chewing improving activity (chewing assisting activity) to the oral composition. The additive may be prepared using an enzyme modified isoquercitrin in combination with a thickening polysaccharide.

There are described compositions comprising an effective amount of a combination of two or more components, said components selected from acacetin, ACTI peptide, alpha-lipolic acid, alprostadil, anisomycin, apigenin, ascorbic acid, astragalus, berberine, β-lapachone, β-hydroxy-beta-methyl-butyrate, Bacopa monnieri, catechin, catechol, chamomile, chrysin, coumestrol, curcumin, dinitrophenol, dinoprost, ellagic acid, (−)-epigallocatechin gallate, green tea extract, fisetin, genistein, ginsenoside RE, glabridin, 18-α-glycyrrhetinic acid, 18-β-glycyrrhetinic acid, glycyrrhizin, hydroquinone, isoquercitrin (EMIQ), kaempferol, kuromanin, leucine, lithium, luteolin, luteolin, luteolinidin, melatonin, menadione, 1-methylnicotinamide (MNA), methyl salicylate, myricetin, nadide, niacin (vitamin B3), nicotinamide (NAM), nicotinamide mononucleotide (NMN), nicotinamide riboside (NR), nicotinic acid adenine dinucleotide (NaAD), nicotinic acid mononucleotide (NaMN), parsley (Petroselinium crispum), phenylephrine, pokeweed mitogen, 15-Δ prostaglandin J2, puromycin, quercetin, quinolinic acid, retinoic acid, trichostatin A, troxrutin, rutin, tryptophan, vitamin D3, withaferin A, wortmannin and zinc (including salts thereof).

The invention provides a method for preparing high-purity isoquercitrin from polygonum viviparum, which comprises the following steps: sequentially carrying out water extraction impurity removal and alcohol extraction on a polygonum viviparum raw material to obtain an alcohol extract; decolorizing the alcohol extract, then adjusting the pH value to 9-11 to precipitate and remove tannin, and then carrying out vacuum concentration to obtain a general flavone crude product; performing acid hydrolysis on the crude product, performing separation and purification by adopting high-pressure preparative liquid chromatography, performing online monitoring by utilizing an ultraviolet detector, and obtaining a fraction rich in isoquercitrin by accurately controlling starting and ending points of fraction collection; and concentrating under reduced pressure, washing with water and drying to obtain the product. By optimizing the pretreatment and chromatographic separation conditions, the purity and yield of the product are synchronously improved, the HPLC (High Performance Liquid Chromatography) purity of the obtained isoquercitrin reaches 98% or above, and the method is low in process operation cost, environment-friendly and suitable for large-scale production.

The present invention provides a saliva secretion stimulating agent having a dosage form usable in the oral cavity or a dosage form usable by oral administration (dosage form for the oral cavity, dosage form for oral administration). The saliva secretion stimulating agent can be prepared using enzyme-modified isoquercitrin or a combination of enzyme-modified isoquercitrin and a thickening polysaccharide. The present invention also provides an additive (additive for oral composition) that is effective for preparing an oral composition (food or beverage, pharmaceutical product for oral administration) and can impart at least one activity selected from the group consisting of saliva secretion stimulating activity, swallowing improvement activity (swallowing aid activity), and mastication improvement activity (mastication aid activity) to the oral composition. The additive can be prepared using a combination of enzyme-modified isoquercitrin and a thickening polysaccharide.

The invention discloses a method for detecting the content of isoquercitrin in a chingma abutilon seed sample and application of the method. A test solution and a reference solution are prepared respectively and injected into an ultra-high performance liquid chromatograph, and specific chromatograms of the reference solution and the test solution are compared; and calculating the content of the isoquercitrin in the chingma abutilon seed sample by utilizing a regression equation of the sample size and the peak area of the isoquercitrin. The method disclosed by the invention is good in specificity and durability, and a new analysis means can be provided for quality detection of a chingma abutilon seed sample; the specific component of isoquercitrin is taken as an index, so that the method is more reasonable and accurate; the method is applicable to detection of chingma abutilon seed medicinal materials, standard decoction, formula granules and other products containing chingma abutilon seeds. The method is simple to operate, good in reproducibility and stability, reliable in recovery rate, low in detection cost, high in detection efficiency and small in environmental pollution.

This invention discloses a method for determining the quality of *Hemiberlesia lingua* stems and leaves. The method includes fingerprint spectroscopy establishment and similarity analysis, content determination, cluster analysis, principal component analysis, and partial least squares discriminant analysis. The method of this invention involves UPLC fingerprint analysis and similarity analysis of 15 batches of *Hemiberlesia lingua* stems and leaves from different origins. A total of 28 common peaks were found, and 7 of these common peaks were identified using reference standards: peak 4 (neochlorogenic acid), peak 8 (chlorogenic acid), peak 9 (caffeic acid), peak 11 (cryptochlorogenic acid), peak 22 (isoquercetin), peak 24 (astragalin), and peak 28 (isochlorogenic acid C). This invention's method is highly sensitive, specific, stable, and feasible, providing a reference for the quality evaluation and control of *Hemiberlesia lingua* stems and leaves.

The present application relates to the application of isoquercitrin in the preparation of a drug for treating post-traumatic stress disorder, and belongs to the technical field of natural chemical medicine development. The present application systematically clarifies the whole-chain mechanism of isoquercitrin in treating PTSD through the "GABA A R-PINK1 / Parkin-mitophagy" pathway by means of a variety of technical means such as molecular docking, microthermal swimming, patch clamp, proteomics, cell and animal models. Compared with the clinically commonly used drugs for treating post-traumatic stress disorder, isoquercitrin, as a natural flavonoid compound, can improve the symptoms of PTSD while not damaging the learning and memory function, and has better safety. Moreover, isoquercitrin is widely sourced and has a mature preparation process. The present application provides a theoretical basis and experimental support for the application of isoquercitrin in the development of drugs for treating post-traumatic stress disorder, and has important clinical application value and industrialization prospects.

The present application relates to the technical field of traditional Chinese medicine analysis, and particularly relates to a mulberry fruit paste fingerprint spectrum establishment method and fingerprint spectrum. The mulberry fruit paste fingerprint spectrum establishment method comprises the following steps: taking mulberry fruit paste as raw material to prepare a test sample solution, taking chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, 5-hydroxymethyl furfural, rutin and isoquercitrin to prepare reference sample solutions; injecting the test sample solution and the reference sample solutions into an ultra-high performance liquid chromatograph for determination, recording chromatograms, collecting chromatograms of test sample solutions of multiple batches of mulberry fruit paste, importing a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, generating a control spectrum, and taking peak area and retention time as parameters to calculate similarity. The method can simultaneously detect multiple components in mulberry fruit paste, and can be effectively used to evaluate product quality stability of mulberry fruit paste.

The invention discloses an application of a pyracantha fortuneana fruit extract in prevention and treatment of ulcerative colitis, and relates to the technical field of food medicines. The main components of the pyracantha fortuneana fruit extract comprise total flavonoids, total polyphenols and total triterpenes. Wherein the total flavonoids and the total polyphenols comprise hyperoside, rutin, isoquercitrin and epicatechin, and the total triterpenes comprise jujubogenin and the like. Functional food, health care products, medicines and other products prepared from the pyracantha fortuneana fruit extract can reduce the disease activity index, increase the colon length, reduce the abnormally-increased pro-inflammatory cytokine TNF-alpha content in serum, improve the glutathione peroxidase activity and achieve prevention and treatment of ulcerative colitis. Therefore, the pyracantha fortuneana fruit extract disclosed by the invention can be used as a raw material for preparing foods and medicines for preventing and treating ulcerative colitis.

The invention discloses a method for producing a flos carthami extract with high isoquercitrin content, which comprises the following steps: 1) extracting flos carthami in a fading period by using methanol, ethanol and / or water; and 2) drying the extracting solution. Research on differential metabolites and differential genes in petals in different flowering stages shows that the content of effective component flavone in safflower in a fading stage is lower than that in safflower in a full-bloom stage, the petal metabolites and genes in the three stages are obviously separated, and samples in the initial flowering stage and the full-bloom stage are close to each other and far away from samples in the fading stage. The content of isoquercitrin in petals in the fading period of the extract extracted by the method is high and can reach 0.164 mg / g, and the content of isoquercitrin in the full-bloom period can reach 0.067 mg / g. The safflower extract with high isoquercitrin content is produced by adopting the fading period safflower, so that the fading period safflower is turned into wealth, and the economic benefit is increased.

The invention discloses a rapid analysis and content determination method for components of an alcohol extract of sedum emarginatum, relates to the technical field of quality control of medicinal materials, and solves the problem that the detection efficiency is low when the contents of various components of sedum emarginatum are determined in the prior art. According to the method, under the same chromatographic condition, six components including schaftoside, isoquercitrin, astragalus smicus glycoside, p-coumaric acid, gallic acid and protocatechuic acid contained in the sedum emarginatum alcohol extract are subjected to high performance liquid chromatography determination at the same time, and the problem that the detection efficiency is low when the content of various components of sedum emarginatum is determined at present is solved. The content of various components such as sedum emarginatum schaftoside, isoquercitrin, astragalus smicus glycoside, p-coumaric acid, gallic acid and protocatechuic acid can be determined under the same high performance liquid chromatography condition, and the determination method is accurate, high in sensitivity, good in repeatability and high in stability.