Chan tablet liquid chromatography-mass spectrometry determination method

A liquid mass spectrometry and determination method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of undisclosed content determination methods and undetectable components

Pending Publication Date: 2022-03-01
NANCHANG JISHUN PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent 201910890137.1 discloses a quality testing method for Hedan tablets, but the method does not disclose the content determination method of active ingredients
Chinese patent 2019108930497 discloses a hig

Method used

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  • Chan tablet liquid chromatography-mass spectrometry determination method
  • Chan tablet liquid chromatography-mass spectrometry determination method
  • Chan tablet liquid chromatography-mass spectrometry determination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Preparation of reference solution

[0086] Take psoralen, different psoralen, hyperoside, quercetin 3-O-glucuronide, isoquercetin, sennoside B, astragalus glycoside, sennoside A, Arnicic acid, shikonian acid, salvianolic acid B, nuciferine, salvianolic acid A, psoralen, isopsoralen, salvianolic acid C, rhein, neopsoralen isoflavones, psoralen Appropriate amounts of reference substances of Ning, cryptotanshinone, and bakuchiol were accurately weighed, placed in 10mL volumetric flasks, dissolved in methanol to constant volume, and prepared into concentrations of 227.6 μg / mL, 128.3 μg / mL, and 169.5 μg / mL, respectively. mL, 248.4μg / mL, 135.7μg / mL, 185.3μg / mL, 119.2μg / mL, 84.2μg / mL, 193.8μg / mL, 74.8μg / mL, 236.1μg / mL, 149.3μg / mL, 106.2μg / mL mL, 196.4μg / mL, 125.8μg / mL, 62.7μg / mL, 174.8μg / mL, 58.3μg / mL, 44.2μg / mL, 138.3μg / mL, 96.4μg / mL stock solutions of reference substances.

[0087] Take psoralen, isopsoralen, hyperoside, isoquercitrin, nuciferine, psoralen, isopsoralen, ne...

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Abstract

The invention relates to a liquid chromatography-mass spectrometry determination method for a Holdan tablet. The method comprises the following steps: mixing psoralen glycoside, isopsoralen glycoside, hyperoside, quercetin 3-O-glucuronide, isoquercitrin, sennoside B, astragalus smicus glycoside, sennoside A, rosmarinic acid, alkannic acid, salvianolic acid B, nuciferine, salvianolic acid A, psoralen, isopsoralen, salvianolic acid C, rhein, neopsoralen isoflavone, psoralen and cryptotanshinone, and uniformly stirring to obtain a mixture; the method comprises the following steps of: preparing reference substance solutions A and B and a test sample solution by taking a sample as a reference substance and bakuchiol as a reference substance, injecting the reference substance solutions A and B and the test sample solution into a liquid chromatograph-mass spectrometer, obtaining mass spectrograms when A is in a positive ion mode and B is in a negative ion mode, and calculating the content of the effective components in the test sample according to the mass spectrograms under the chromatographic conditions that an Agilent C18 chromatographic column (4.6 mm * 250 mm, 5 [mu] m), the flow rate is 0.4 mL.min <-1 > and the sample injection volume is 2 [mu] L. The column temperature is 30 DEG C, and a 0.2% phosphoric acid aqueous solution (A)-acetonitrile (B) is used as a mobile phase for gradient elution.

Description

technical field [0001] The invention relates to a detection method of a Chinese patent medicine, in particular to a liquid-mass spectrometry detection method of Hedan tablets. Background technique [0002] Hedan Tablet is a Chinese patent medicine that has been on the market. It is composed of lotus leaf, salvia miltiorrhiza, hawthorn, senna leaf, and psoralen (stir-fried with salt). It has the effects of resolving phlegm and reducing turbidity, promoting blood circulation and removing blood stasis. For hyperlipidemia caused by turbid phlegm and blood stasis. Chinese patent 201910890137.1 discloses a quality testing method for Hedan tablets, which does not disclose the content determination method of the active ingredients. Chinese patent 2019108930497 discloses a high-performance liquid chromatography method for determining the content of various active ingredients in Hedan tablets, but the three ingredients of sennoside B, sennoside A, and salvianolic acid C cannot be de...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72G01N2030/027
Inventor 曾英姿周万辉赵磊
Owner NANCHANG JISHUN PHARMA CO LTD
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