Chan tablet liquid chromatography-mass spectrometry determination method

Abstract

The invention relates to a liquid chromatography-mass spectrometry determination method for a Holdan tablet. The method comprises the following steps: mixing psoralen glycoside, isopsoralen glycoside, hyperoside, quercetin 3-O-glucuronide, isoquercitrin, sennoside B, astragalus smicus glycoside, sennoside A, rosmarinic acid, alkannic acid, salvianolic acid B, nuciferine, salvianolic acid A, psoralen, isopsoralen, salvianolic acid C, rhein, neopsoralen isoflavone, psoralen and cryptotanshinone, and uniformly stirring to obtain a mixture; the method comprises the following steps of: preparing reference substance solutions A and B and a test sample solution by taking a sample as a reference substance and bakuchiol as a reference substance, injecting the reference substance solutions A and B and the test sample solution into a liquid chromatograph-mass spectrometer, obtaining mass spectrograms when A is in a positive ion mode and B is in a negative ion mode, and calculating the content of the effective components in the test sample according to the mass spectrograms under the chromatographic conditions that an Agilent C18 chromatographic column (4.6 mm * 250 mm, 5 [mu] m), the flow rate is 0.4 mL.min <-1 > and the sample injection volume is 2 [mu] L. The column temperature is 30 DEG C, and a 0.2% phosphoric acid aqueous solution (A)-acetonitrile (B) is used as a mobile phase for gradient elution.

Description

technical field [0001] The invention relates to a detection method of a Chinese patent medicine, in particular to a liquid-mass spectrometry detection method of Hedan tablets. Background technique [0002] Hedan Tablet is a Chinese patent medicine that has been on the market. It is composed of lotus leaf, salvia miltiorrhiza, hawthorn, senna leaf, and psoralen (stir-fried with salt). It has the effects of resolving phlegm and reducing turbidity, promoting blood circulation and removing blood stasis. For hyperlipidemia caused by turbid phlegm and blood stasis. Chinese patent 201910890137.1 discloses a quality testing method for Hedan tablets, which does not disclose the content determination method of the active ingredients. Chinese patent 2019108930497 discloses a high-performance liquid chromatography method for determining the content of various active ingredients in Hedan tablets, but the three ingredients of sennoside B, sennoside A, and salvianolic acid C cannot be de...

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Problems solved by technology

Chinese patent 201910890137.1 discloses a quality testing method for Hedan tablets, but the method does not disclose the content determination method of active ingredients

Chinese patent 2019108930497 discloses a high-performance liquid chromatography method for determining the content of various active ingredients in Hedan tablets, but the three ingredients of sennoside B, sennoside A, and salvianolic acid C cannot be detected

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