Carrier capable of simultaneously improving rosmarinic acid and salvianolic acid B content of red sage root and use thereof

Abstract

The invention discloses a carrier capable of simultaneously improving rosmarinic acid and salvianolic acid B content of red sage root and a use thereof. The carrier contains an over-expressed AtPAP1 transcription factor and can realize RNA interference of a SmCOMT gene. Dried root of three-month-old of the transgenic red sage root plant obtained by the carrier has rosmarinic acid content of 9.59+ / -0.36mg / g which is 1.15 times that of dried root of the same old of the common red sage root plant, and salvianolic acid B content of 55.87+ / -0.59mg / g which is 1.79 times that of the dried root of the same old of the common red sage root plant.

Description

technical field [0001] The invention belongs to the technical field of medicinal plant genetic engineering, and specifically relates to a carrier capable of simultaneously increasing the contents of rosmarinic acid and salvianolic acid B in salvia miltiorrhiza and application thereof. Background technique [0002] Salvia ( Salvia miltiorrhiza Bunge) is a perennial herb of the genus Salvia in the Labiatae family. It is used as medicine with its roots and rhizomes, and is one of the important bulk medicinal materials. Salvia miltiorrhiza has the effects of promoting blood circulation and removing blood stasis, stimulating menstrual flow, relieving pain, clearing heart and eliminating troubles, etc. It is mainly used in the treatment of angina pectoris, hyperlipidemia, ischemic stroke, hepatitis, acne and other diseases in clinical practice, and has achieved good curative effect. The main active ingredients of Danshen can be divided into fat-soluble tanshinones and water-solub...

AI Technical Summary

Problems solved by technology

Medicinal plants have strict requirements on soil, climate, altitude and other cultivation conditions, and the land area suitable for artificial cultivation is very limited. In order to meet market demand, continuous cropping followed, which led to aggravation of diseases and insect pests in the cultivation process of Salvia miltiorrhiza, and physiological dead seedlings It keeps happening, threatening the sustainable development of the main production areas of Danshen

At the same time, the lack of systematic breeding and purification and rejuvenation of cultivated Danshen leads to mixed germplasm resources of Danshen, different contents of medicinal ingredients, and degeneration of varieties, which seriously affects the quality and yield of Danshen

The above problems have become the bottleneck restricting the development of Danshen medicinal material industry

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