Method for pre processing sample in use for measuring concentration of salvianolic acid B, in biologic body fluid

A biological sample, concentration determination technology, applied in the pharmacokinetic research of traditional Chinese medicine, the pharmacokinetic research field of salvianolic acid B and traditional Chinese medicine containing salvianolic acid B, can solve the problem of low sensitivity, insufficient sensitivity of concentration determination method, Unable to detect problems such as blood drug concentration, to achieve the effect of improving sensitivity

Inactive Publication Date: 2007-05-09
SHANGHAI UNIV OF T C M
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Problems solved by technology

Therefore, there is insufficient understanding of the pharmacokinetics of salvianolic acid B and salvianolic acid B in Danshen preparations, which cannot provide a basis for the development of new salvianolic acid B drugs and guide clinical rational drug use.
[0005] In addition, since salvianolic acid B is a water-soluble component, when the concentration in the sample is low, it is difficult to fully extract salvianolic acid B in the sampl...

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Embodiment Construction

[0013] A pretreatment method for measuring the concentration of salvianolic acid B in biological fluids is to mix biological samples containing salvianolic acid B concentration (including plasma, urine, tissue homogenate supernatant, etc.) with 2.17 mol / L phosphoric acid. After homogenization, the volume ratio between biological sample and phosphoric acid is 1:2-3; then add the Waters Oasis HLB cartridge that has been activated with methanol and water. After elution and elution, the eluent is at 25℃ Under the conditions of evaporation and enrichment, the mobile phase of 1% trifluoroacetic acid: methanol: acetonitrile (70:10:20, v:v:v) can be reconstituted by high performance liquid chromatography (chromatographic conditions: analytical column For Thermo Hypersil-Keystone ODSHypersil C 18 Column, mobile phase is 1% trifluoroacetic acid: methanol: acetonitrile (70:10:20, v:v:v), flow rate is 1m1·min -1 , The column temperature is 30°C, and the detection wavelength is 315nm) for dete...

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Abstract

A method for pre-treating analysis sample of red phenolic acid B concentration in body fluid on living beings includes utilizing water- methanol to spray-wash then utilizing aqueous ammonia-methauol to elute Waters Oasis HLB small column obtained by activating sample containing red phenolic acid with water and methanil, enriching eluted liquid, using high efficiency of chromatography to carry out pretreatment on living beings sample containing red phenolic acid B after enriched matter is redissloved by flowing phase of 1% trifluoroacetic acid: methanol: acetonitrile.

Description

Technical field [0001] The invention relates to the pharmacokinetics research field of traditional Chinese medicines, in particular to the pharmacokinetics research field of salvianolic acid B and Chinese medicines containing salvianolic acid B, in particular to a pretreatment method for measuring the concentration of salvianolic acid B in biological fluids. Background technique [0002] Salvia miltiorrhiza is one of the most commonly used Chinese medicinal materials. The research results show that the water-soluble components are mainly responsible for promoting blood circulation and removing blood stasis, while salvianolic acid B has the highest content of water-soluble components and the strongest activity of activating blood circulation and removing blood stasis. The National Pharmacopoeia Commission designated salvianolic acid B as the quality control indicator for Danshen tablets in March 2002. Furthermore, salvianolic acid B may become a quality control indicator for Dansh...

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Application Information

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IPC IPC(8): G01N30/08G01N30/02B01J20/281
Inventor 马越鸣王天明
Owner SHANGHAI UNIV OF T C M
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