34results about How to "Symmetrical peak shape" patented technology

The invention discloses a high performance liquid chromatography (HPLC) detection method of Rivaroxaban. The method comprises the following steps: a. preparing a test solution; b. preparing a reference solution; c. respectively detecting the test solution and the reference solution by adopting HPLC, wherein the detection conditions are as follow: the stationary phase of a chromatographic column is silica gel which is coated with amylose-tri ((S)-alpha-methyl phenyl carbamate) on the surface, the mobile phase of the chromatographic column is acetonitrile-water, the flow velocity is 0.5-1.5ml / min, the column temperature is 10-40 DEG C, and the detection wave length is 200nm-300nm. After the detection method is used, the degree of separation of Rivaroxaban and Rivaroxaban R isomer is more than 3.0, and more theoretical plates are available, so that the detection result accuracy is effectively prevented from being influenced by the interference of all components; furthermore, the detection method has the advantages of being accurate and reliable in detection results, short in analysis time, low in cost, simple and convenient in operation, etc.

The invention discloses a method for separating and measuring empagliflozin and its related substances. The method adopts high performance liquid chromatography, uses octylsilane bonded silica gel packing or phenylsilane bonded silica gel packing as a chromatographic column, and uses Phosphoric acid solution, methanol and acetonitrile constitute the mobile phase for gradient elution. The method can well separate empagliflozin and its related substances, has good detection reproducibility, high sensitivity, strong specificity, and simple operation, and is helpful for controlling the quality and drug safety of empagliflozin.

Belonging to the field of pharmaceutical analysis, the invention relates to a separation and detection method for related substances in an ophthalmic gel. The method includes: preparing a to-be-detected solution, performing sample introduction, and conducting separation under the following conditions that: the chromatographic column is octadecyl silane bonded silica gel, the mobile phase is an acetonitrile-buffer solution and acetonitrile, the volume content of the mobile phase B is: 0% at 0-18min; 0-30% at 18-25min; 30% at 25-39min; 30-0% at 39-40min; and 0% at 40-50min; and conducting detection. The method can effectively separate related substances in the to-be-detected material. The control solutions of the related substances are prepared, the same method is adopted for high performance liquid chromatographic analysis, the peak area is read, and the content of each substance in the to-be-detected material is calculated. The method can rapidly and accurately determine the content of related substances in the to-be-detected material simultaneously, and is convenient for control of the product quality.

The invention provides a method for detecting solid potassium dimethyldithiocarbamate purity by a liquid phase chromatography method. According to the method, mobile phases are composed of a mobile phase a, a mobile phase b and a mobile phase c; the mobile phase c is an alkali organic amine compound. Derivation or preprocessing is not needed before liquid phase analysis is conducted on a sample, and sample analysis is directly carried out after dissolving and deaeration are conducted; in an obtained liquid phase chromatogram, all impurity peaks in the sample are detected, the separation effectbetween a main peak and the impurity peaks is good, the peak value is normal, the peak shape of the main peak is symmetric, smearing does not exist, and the detection time is short.

The invention discloses a method for detecting isomers of camphorsulfonic acid or its salts. The detection method uses high performance liquid chromatography to qualitatively or quantitatively detect the isomers of camphorsulfonic acid or its salts. The detection conditions include: Chromatographic column: ion exchange column; detection wavelength: 210~400nm; mobile phase: organic phase containing acid and diethylamine, the acid is formic acid or acetic acid, and the detection method also includes the following steps: (1) preparing the Test solution, reference substance solution; (2) need test solution, reference substance solution sample injection detection respectively; Described need test solution is the isomer solution of camphorsulfonic acid or its salt, preferably L-camphorsulfonic acid solution or / and D-camphorsulfonic acid solution, more preferably L-camphorsulfonic acid solution. Adopting the method of the invention has good separation degree and high theoretical plate number.

The present invention relates to a preparation method of a novel grafted high-capacity dendritic macromolecular ion chromatographic stationary phase filler, and provides a preparation method for a grafted high-capacity dendritic macromolecular ion chromatographic stationary phase, including an integer generation dendritic macromolecule Preparation, preparation of polystyrene-divinylbenzene-glycidyl methacrylate (PS-DVB-GMA) microspheres, grafting of dendrimers, quaternization of dendrimers and high-capacity dendrimers Preparation of macromolecule grafted anion chromatography packing. This method makes full use of a large number of epoxy groups on the surface of PS-DVB-GMA microspheres and a large number of terminal amine groups of integer-generation dendrimers to modify the surface of the microspheres. The reaction is fast, the cycle is short, the method is simple, and the synthesized The packing exchange capacity is high, the prepared ion chromatographic column has good resolution and high peak shape symmetry, and can be used for the separation of fluoride ions, organic acids and sugars.

The invention relates to a method for measuring hydrogen sulfide content in desulfurized amine, which is used for measuring the hydrogen sulfide content in the desulfurized amine for refining and chemical enterprises. The method comprises the following steps of: adding a sample into a headspace bottle, sealing, adding dilute acid, shaking uniformly, and reacting the dilute acid with the sample to separate out hydrogen sulfide; standing, cooling to room temperature, performing chromatographic analysis on a headspace gas-phase component, and detecting by using a thermal conductivity cell detector; and calculating the hydrogen sulfide content in a barren solution in the desulfurized amine by using hydrogen sulfide standard gas configuration and a standard liquid calibrator according to the hydrogen sulfide content in a standard liquid, a peak area of the headspace gas-phase component hydrogen sulfide, and a peak area of a headspace gas-phase component hydrogen sulfide of the sample. By the method, the analysis is quick, and a chromatographic column and an injection port are prevented from being polluted by the sample and impurities, so that a chromatogram baseline is stable, peak shapes of hydrogen sulfide are symmetric, the interference of other substances on analysis is eliminated, and the analysis accuracy is improved.

The invention discloses a carrier coating device of a capillary chromatography base pipe. The carrier coating device comprises an air inlet, an air inlet valve, a flow speed control valve and a copper pressure-resisting pipe which are in sealed connection in sequence through pipelines, wherein the pipeline between the flow speed control valve and the copper pressure-resisting pipe is further connected with an exhausting valve and a pressure gauge; the copper pressure-resisting pipe is provided with a cavity and a sealing cover; and the sealing cover is provided with a leading-in pipe for inserting the capillary chromatography base pipe. The invention further provides a method for coating the capillary chromatography base pipe with a carrier, so that ion liquid coating is well performed, and the coating effect of the ion liquid with middle and strong polarities is enhanced, and furthermore, the separation capability of fixed phases for some complicated samples is improved.

The invention discloses a method for measuring residual amount of tetrabutylammonium bromide in an organic drug. The method is characterized in that the liquid chromatography is adopted, a reversed phase column is selected, and a mobile phase is formed by acetonitrile and a tetramethylammonium hydroxide and phosphoric acid solution for gradient elution. Compared with the prior art, the method provided by the invention achieves better separation of the tetrabutylammonium bromide from the organic drug, high test reproducibility, sensitivity and specificity, as well as simplicity and convenience in operation, and is conductive to the control of quality and usage safety for the organic drug.

The present invention specifically relates to a method for detecting ethylenediamine in lipoic acid injection, comprising the following contents: 1) taking ethylenediamine to prepare a reference substance solution; 2) taking zinc sulfate injection to prepare a test solution; 3) using ion Chromatography detects reference substance solution and need testing solution respectively, calculates ethylenediamine content; Wherein the chromatographic condition of ion chromatography comprises the following content: Chromatographic column: stationary phase is cation exchange resin or chemically bonded ion exchanger; Mobile phase: mobile phase Phase A is methanesulfonic acid, mobile phase B is methanol or acetonitrile; the volume ratio of mobile phase A and mobile phase B is 90-98:10-2. The ion chromatography method of the invention is used to detect ethylenediamine, which can effectively detect ethylenediamine, and the detection result is accurate, the baseline is flat, the peak shape is symmetrical, the stability is high, the repeatability is good, and the sensitivity is high.

A method for determining peramivir intermediate isomers by high performance liquid chromatography, characterized in that: the adopted chromatographic column is a polysaccharide derivative-coated chiral chromatographic column; the adopted mobile phase is isopropanol and n-hexane The mixed solution of alkane is eluted by isocratic in the high performance liquid chromatography system; in the mobile phase, the volume ratio of isopropanol and n-hexane is 10:90~20:80; the flow rate of the mobile phase is 0.8 ~1.0 ml / min; the detector used is an ultraviolet detector with a monitoring wavelength of 215 nm. The invention overcomes the deficiencies of the prior art, solves the problem of analysis and determination of peramivir intermediate isomers, can effectively control the impurity content of the target product and its isomers, and avoids the interference of isomer impurities on subsequent synthesis reactions , improve the quality of the subsequent preparation of peramivir, and ensure the safety of medication. The invention provides an accurate and efficient detection method for the isomer determination of peramivir intermediates.

The invention provides a high pH tolerance chromatographic filler and a preparation method thereof. The filler is prepared by using ultra-pure entire porous spherical silica gel as matrix, and coating a high pH tolerance inorganic / organic hybrid layer with the thickness of several nm through a reaction for surface modification in advance. The filler not only keeps the advantages of other silica-based fillers, but also overcomes the defects of narrow pH range of the traditional silica-based fillers, so as to broaden the PH range to 1-12.5. In the preparation of the filler, an inorganic / organichybrid polymer is prefabricated, then the inorganic / organic hybrid polymer is reacted with the silica gel matrix, and the hybrid layer is coated on the silica gel matrix.

The invention belongs to the technical field of traditional Chinese medicine injection detection, and in particular relates to a detection method and application of acetic acid, propionic acid and butyric acid in a traditional Chinese medicine injection. The detection method comprises: diluting the traditional Chinese medicine injection with a hydrochloric acid-ethanol mixed solution, Acetic acid, propionic acid and butyric acid in the traditional Chinese medicine injection are detected by gas chromatography, and the detection method is an internal standard method. The detection method can rapidly and accurately detect the contents of acetic acid, propionic acid and butyric acid in traditional Chinese medicine injections simultaneously, has strong specificity and good reproducibility, and can make up for the deficiencies of existing standards and other quantitative methods. Through the detection method, the content determination of the three components in Shuxuetong injection can be carried out quickly and accurately at the same time, which is of great significance for improving the quality standard of Shuxuetong injection.

Belonging to the technical field of pharmaceutical analysis, the invention in particular relates to a method for detection of metadoxine related substances by high performance liquid chromatography. A metadoxine test solution and a reference solution are detected by high performance liquid chromatography respectively, chromatograms of the two are recorded, a peak area of vitamin B6 in the metadoxine test solution and a main peak area of the reference solution are acquired, and quantitative analysis is carried out on metadoxine related substances by external standard method. The reference solution is a pure metadoxine solution. The method provided by the invention has the advantages of high separation degree, moderate principal component retention time, i.e. short analysis time, and low cost.