34results about How to "Symmetrical peak shape" patented technology

The invention belongs to the field of pharmaceutical analysis and specifically relates to a method for detecting impurity phenylhydrazine in edaravone. The invention aims to provide a conveniently operated, quick and accurate method for detecting the phenylhydrazine. The method comprises the following steps of: controlling a key HPLC (High Performance Liquid Chromatography) detecting condition by adopting liquid chromatogram detection, wherein for a moving phase, a volume percent of acetate buffer solution to carbinol is (70%-90%):(10%-30%) or the volume percent of acetate buffer solution to acetonitrile is (70%-90%):(10%-30%); the concentration of the acetate buffer solution is 0.04-0.06mol/L and pH value is 6.4-6.8; the detection wavelength is 226-238nm; the flow speed is 0.6-1.2ml/min; and the column temperature is 10-35 DEG C. The key detection parameters are controlled, so that the convenient, quick and accurate detecting purpose is achieved. The method provided by the invention has the advantages of convenience for operation accuracy and reliability in detecting result, stronger specificity, and shorter detection time because the main peak retention time is 9 minutes, and provides a brand new choice for detecting the impurity and controlling the product quality.

The invention discloses a method for measuring Jardiance and related substances of Jardiance through separation. According to the method, gradient elution are performed by adopting high performance liquid chromatography, utilizing octyl silane bonded silica gel filler or phenyl silane bonded silica gel filler as a chromatographic column and adopting a moving phase formed by a phosphoric acid solution, methyl alcohol and acetonitrile. With the adoption of the method, Jardiance and related substances of Jardiance can be separated very well, the detection is good in reproducibility, high in sensitivity and specificity and simple to operate, and control for quality and medication safety of Jardiance is facilitated.

The invention discloses a method for measuring residual amount of tetrabutylammonium bromide in an organic drug. The method is characterized in that the liquid chromatography is adopted, a reversed phase column is selected, and a mobile phase is formed by acetonitrile and a tetramethylammonium hydroxide and phosphoric acid solution for gradient elution. Compared with the prior art, the method provided by the invention achieves better separation of the tetrabutylammonium bromide from the organic drug, high test reproducibility, sensitivity and specificity, as well as simplicity and convenience in operation, and is conductive to the control of quality and usage safety for the organic drug.

The invention discloses a method for rapidly measuring patulin in fruit jam and vegetable paste, comprising the following steps: weighing 2.00g of fruit jam and vegetable paste in a centrifuge tube, adding 0.5-3mL of water, shaking, adding 5mL of ethyl acetate-normal hexane solution (95+5, v/v), shaking, simultaneously adding 0.5-2g of sea sands, 4-8g of anhydrous sodium sulfate and 0.5-2g of sodium bicarbonate, shaking vigorously for 2min, and centrifuging for 3min; and weighing 4mL of supernate until ethyl acetate activates a carbon black columella, receipting eluent by a test tube in which glacial acetic acid is added, eluting by 3-7mL of ethyl acetate, and drying; dissolving 240 mu L of acetonitrile, pipetting 150 mu L of dissolution liquid into a sample bottle, adding 50 mu L of derivatization reagent, heating by microwave for 1-7min, cooling and then measuring by gas chromatography-mass spectrometry. The method is rapid, simple, sensitive, accurate, specific and durable in confirmation and quantification, and is widely applied to the measurement filed of patulin in fruit jam and vegetable paste.

The invention relates to a method for determining peramivir intermediate isomers by using high performance liquid chromatography. The method is characterized in that an adopted chromatographic columnis a polysaccharide derivative coated chiral chromatographic column; the adopted mobile phase is a mixed solution of isopropanol and normal hexane, and isocratic elution is adopted in a high performance liquid chromatography system; in the mobile phase, the volume ratio of isopropanol to normal hexane is 10: 90 to 20: 80; the flow velocity of the mobile phase is 0.8 to 1.0 ml/min; an adopted detector is an ultraviolet detector, and the monitoring wavelength is 215 nm. The method overcomes the defects in the prior art, solves the problem of analytical determination of the peramivir intermediateisomer, can effectively control the contents of the target product and isomer impurities thereof, avoids the interference of the isomer impurities on the subsequent synthesis reaction, enhances the quality of the subsequent prepared peramivir, and ensures the medication safety. The invention provides an accurate and efficient detection method for determining the isomer of the peramivir intermediate.

The invention discloses a high performance liquid chromatography (HPLC) detection method of Rivaroxaban. The method comprises the following steps: a. preparing a test solution; b. preparing a reference solution; c. respectively detecting the test solution and the reference solution by adopting HPLC, wherein the detection conditions are as follow: the stationary phase of a chromatographic column is silica gel which is coated with amylose-tri ((S)-alpha-methyl phenyl carbamate) on the surface, the mobile phase of the chromatographic column is acetonitrile-water, the flow velocity is 0.5-1.5ml/ min, the column temperature is 10-40 DEG C, and the detection wave length is 200nm-300nm. After the detection method is used, the degree of separation of Rivaroxaban and Rivaroxaban R isomer is more than 3.0, and more theoretical plates are available, so that the detection result accuracy is effectively prevented from being influenced by the interference of all components; furthermore, the detection method has the advantages of being accurate and reliable in detection results, short in analysis time, low in cost, simple and convenient in operation, etc.

The invention belongs to the technical field of traditional Chinese medicine injection detection, and particularly relates to a detection method and application of acetic acid, propionic acid and butyric acid in a traditional Chinese medicine injection. The detection method comprises the steps that the traditional Chinese medicine injection is diluted with an ethanol hydrochloride mixed solution,and acetic acid, propionic acid and butyric acid in the traditional Chinese medicine injection are detected through gas chromatography; the detection method is an internal standard method. By means ofthe method, the content of acetic acid, propionic acid and butyric acid in the traditional Chinese medicine injection can be detected simultaneously, quickly and accurately, the specificity is high,the reproducibility is good, and the defects of existing standards and other quantitative methods can be overcome. By means of the method, content determination of the three components in the Shuxuetong injection can be conducted quickly, accurately and simultaneously, and the method has great significance for improving the quality standard of the Shuxuetong injection.

The invention particularly relates to a method for detecting a content of ethylenediamine in medicine by HPLC. The method is mainly realized by performing a derivatization reaction on ethylenediaminewithout ultraviolet absorption to obtain a derivative product with ultraviolet absorption. According to the method, the content of ethylenediamine is detected by adopting high performance liquid chromatography, and the method is accurate in detection result, flat in baseline, symmetrical in peak shape, high in stability, good in repeatability, high in separation degree, low in detection limit, high in sensitivity and free of damage to instruments.

The invention discloses a method of detecting the content of lincomycin hydrochloride in a feed through a high performance liquid chromatography method. A waters 2695 high performance liquid chromatograph is employed with a C18 chromatographic column; a mobile phase includes a 0.05 mol/L borax solution and methanol according to ratio of 55:45; flow rate is 1.0 ml/min; detection wavelength is 214 nm. The method has good separation between a main peak and adjacent peaks, in the range of 14.7-492 [mu]g/ml, the lincomycin hydrochloride can form good linearity, R2 = 0.9987 (n=6). The method is quick, sensitive and reliable, can be used for content detection of the lincomycin hydrochloride in a feed additive. The LC-MS/MS method can supply structural information of a to-be-detected substance; the method is high in sensitivity and selectivity and is an excellent method of detecting various antibiotics. The method is quick, sensitive, reliable and accurate.

The invention relates to a method for characterization of graphene epitaxially grown on SiC based on Ag-particle Raman enhancement effect. The method comprises the following steps: uniformly spraying Ag particles onto a quasi-freestanding graphene wafer at first, and then carrying out testing by using microscopic Raman spectroscopy so as to learn about the situation of bonding of a Si-H bond. According to the invention, the intercalation degree of hydrogen atoms can be directly determined by directly characterizing the Si-H bond in virtue of the Ag-particle Raman enhancement effect; influence of experimental conditions on hydrogen intercalation effect is visually fed back by analyzing the proportion of the Si-H bond in a testing area; and during visual characterization of the Si-H bond, the characteristic G peak, 2D peak and defect D peak of graphene are enhanced to certain extents, which greatly helps further accurate analysis of the structure and the properties like defects of graphene.

The invention discloses a method for separating and determining genotoxic impurities vinyl acetate and benzene in flurbiprofen axetil. According to the method, liquid chromatography is adopted, an octadecyl silica gel chromatographic column is used as a stationary phase, acetonitrile-phosphoric acid-water is used as a mobile phase, vinyl acetate and benzene can be effectively separated, all impurities are good in peak shape, the column efficiency is high, specificity is good, and the quality of flurbiprofen axetil is well controlled.