Detection method and application of acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection

A detection method and injection technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of non-detection of acetic acid, propionic acid and butyric acid content, etc., and achieve shortened detection time, strong specificity, and good reproducibility Effect

Active Publication Date: 2019-04-26
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE +1
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  • Abstract
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Problems solved by technology

[0004] Aiming at the problem that the prior art does not detect the content of acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection, the present invention provides a detection method for acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection

Method used

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  • Detection method and application of acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection
  • Detection method and application of acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection
  • Detection method and application of acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection

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Embodiment 1

[0034] The embodiment of the present invention provides a method for detecting acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection, which are specifically as follows:

[0035] The gas chromatograph is a GC-7890B gas chromatograph from Agilent Company, the elastic quartz capillary column is a DB-FFAP capillary column (30m×0.25mm×0.25μm) from Agilent Company, and the hydrochloric acid is 12N hydrochloric acid.

[0036] 1) Chromatographic conditions: Chromatographic column: DB-FFAP elastic quartz capillary column; heating program: initial temperature 50℃ for 1 min, 15℃ / min to 120℃ and then for 6min, 7℃ / min to 135℃ for 1min ,Rise to 230℃ at 100℃ / min and keep it for 7min; temperature of injection port: 240℃; temperature of detector (FID): 240℃; sample volume: 3μL; split ratio 10:1; carrier gas: high purity nitrogen , Purity>99.999%, flow rate 1.0mL / min; makeup gas: high-purity nitrogen, purity>99.999%, flow rate 25mL / min; hydrogen flow rate 30mL / min, a...

Embodiment 2

[0043] The embodiment of the present invention provides an application of a method for detecting acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection in analyzing the content of acetic acid, propionic acid and butyric acid in Shuxuetong injection, which are specifically as follows:

[0044] The gas chromatograph, elastic quartz capillary column, and chromatographic conditions were the same as in Example 1. The content of acetic acid, propionic acid and butyric acid in Shuxuetong injection was calculated using the linear regression equation of the ratio of the peak area of ​​the reference substance to the peak area of ​​the internal standard compound in Table 1.

[0045] 1) Preparation method of Shuxuetong sample solution: take 0.5mL of Shuxuetong injection sample and place it in a centrifuge tube, add 1.5mL hydrochloric acid and ethanol mixed solution containing internal standard compound (the volume percentage concentration of hydrochloric acid in ...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine injection detection, and particularly relates to a detection method and application of acetic acid, propionic acid and butyric acid in a traditional Chinese medicine injection. The detection method comprises the steps that the traditional Chinese medicine injection is diluted with an ethanol hydrochloride mixed solution,and acetic acid, propionic acid and butyric acid in the traditional Chinese medicine injection are detected through gas chromatography; the detection method is an internal standard method. By means ofthe method, the content of acetic acid, propionic acid and butyric acid in the traditional Chinese medicine injection can be detected simultaneously, quickly and accurately, the specificity is high,the reproducibility is good, and the defects of existing standards and other quantitative methods can be overcome. By means of the method, content determination of the three components in the Shuxuetong injection can be conducted quickly, accurately and simultaneously, and the method has great significance for improving the quality standard of the Shuxuetong injection.

Description

Technical field [0001] The invention belongs to the technical field of traditional Chinese medicine injection detection, and in particular relates to a detection method and application of acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection. Background technique [0002] Shuxuetong injection has the effects of promoting blood circulation, removing blood stasis, and clearing the channels and collaterals. It is often used in the acute stage of ischemic stroke caused by blood congestion and obstruction of the collaterals in the meridian. The symptoms are half-length, crooked tongue and astringent language. The raw materials of Shuxuetong injection are leeches and earthworms. The 2015 edition of the "People's Republic of China Pharmacopoeia" stipulates that the quality of leeches medicinal materials is evaluated by antithrombin activity under the "content determination"; earthworm medicinal materials are currently There is no clear quality evaluation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/04G01N30/68G01N30/86
CPCG01N30/02G01N30/04G01N30/06G01N30/30G01N30/68G01N30/8634G01N2030/045
Inventor 李灵云李振国王跃飞杜宇生倪开岭郝明王丹丹何子龙贾力夫
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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