Method for detecting content of ethylenediamine in medicine by HPLC
A technology of ethylenediamine and content, applied in the field of detection, can solve the problems of mucous membrane and skin irritation, contact dermatitis, corrosion, etc., and achieve the effect of accurate detection results, symmetrical peak shape and good repeatability
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[0038] The experimental process of this application is as follows:
[0039] 1. Analysis method establishment
[0040] Chromatographic conditions to be verified:
[0041] Use octadecylsilane bonded silica gel as filler (Agilent ZORBAX SB C18: 4.6mm×250mm, 5μm or equivalent chromatographic column), and use 5mmol / L ammonium acetate buffer (adjust pH to 6.5 with acetic acid) as the flow In phase A, acetonitrile is used as mobile phase B, and gradient elution is performed; the flow rate is 1.0ml per minute; the detection wavelength is 228nm; the column temperature is 40°C.
[0042] Perform gradient elution as follows:
[0043]
[0044] Preparation assay method:
[0045] Precisely measure 1.0ml of this product, put it in a 100ml measuring bottle, add 9ml of 0.1mol / L hydrochloric acid, 10ml of 0.25mol / L sodium bicarbonate solution (pH=10) and p-toluenesulfonyl chloride solution (take an appropriate amount of p-toluenesulfonyl chloride , add acetone to dissolve and dilute to ma...
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