A kind of detection method and application of acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection

A detection method and injection technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of non-detection of acetic acid, propionic acid and butyric acid content, etc., achieve shortened detection time, improved separation, and good reproducibility Effect

Active Publication Date: 2021-08-10
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE +1
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Problems solved by technology

[0004] Aiming at the problem that the prior art does not detect the content of acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection, the present invention provides a detection method for acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection

Method used

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  • A kind of detection method and application of acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection
  • A kind of detection method and application of acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection
  • A kind of detection method and application of acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection

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Embodiment 1

[0034] The embodiment of the present invention provides a kind of detection method of acetic acid, propionic acid and butyric acid in Chinese medicine injection, specifically as follows:

[0035] The gas chromatograph is GC-7890B gas chromatograph of Agilent Company of the United States, the elastic quartz capillary column is a DB-FFAP capillary column (30m×0.25mm×0.25μm) of Agilent Company of the United States, and the hydrochloric acid is 12N hydrochloric acid.

[0036] 1) Chromatographic conditions: Chromatographic column: DB-FFAP elastic quartz capillary column; heating program: initial temperature of 50°C for 1min, then rise to 120°C at 15°C / min and then hold for 6min, then rise to 135°C at 7°C / min and hold for 1min , rise to 230°C at 100°C / min and keep for 7min; inlet temperature: 240°C; detector (FID) temperature: 240°C; injection volume: 3μL; split ratio 10:1; carrier gas: high-purity nitrogen , purity>99.999%, flow rate 1.0mL / min; makeup gas: high-purity nitrogen, pur...

Embodiment 2

[0043] The embodiment of the present invention provides the application of a detection method for acetic acid, propionic acid and butyric acid in traditional Chinese medicine injection in analyzing the content of acetic acid, propionic acid and butyric acid in Shuxuetong injection, specifically as follows:

[0044] Gas chromatograph, elastic quartz capillary column, and chromatographic conditions are the same as in Example 1. The content of acetic acid, propionic acid and butyric acid in Shuxuetong injection was calculated by using the linear regression equation of the ratio of the peak area of ​​the reference substance to the peak area of ​​the internal standard compound in Table 1.

[0045] 1) Preparation method of Shuxuetong sample solution: Take 0.5mL of Shuxuetong injection sample and place it in a centrifuge tube, add 1.5mL of hydrochloric acid ethanol mixed solution containing internal standard compound (the volume percent concentration of hydrochloric acid in the soluti...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine injection detection, and in particular relates to a detection method and application of acetic acid, propionic acid and butyric acid in a traditional Chinese medicine injection. The detection method comprises: diluting the traditional Chinese medicine injection with a hydrochloric acid-ethanol mixed solution, Acetic acid, propionic acid and butyric acid in the traditional Chinese medicine injection are detected by gas chromatography, and the detection method is an internal standard method. The detection method can rapidly and accurately detect the contents of acetic acid, propionic acid and butyric acid in traditional Chinese medicine injections simultaneously, has strong specificity and good reproducibility, and can make up for the deficiencies of existing standards and other quantitative methods. Through the detection method, the content determination of the three components in Shuxuetong injection can be carried out quickly and accurately at the same time, which is of great significance for improving the quality standard of Shuxuetong injection.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicine injections, in particular to a detection method and application of acetic acid, propionic acid and butyric acid in traditional Chinese medicine injections. Background technique [0002] Shuxuetong Injection has the effect of promoting blood circulation, removing blood stasis, dredging meridians and activating collaterals. It is often used in the acute stage of ischemic stroke caused by congestion and obstruction of collaterals. The raw materials of Shuxuetong injection are leeches and earthworms. The 2015 edition of the Pharmacopoeia of the People's Republic of China stipulates that under the item of "content determination" of leech medicinal materials, the quality of leech medicinal materials is evaluated by antithrombin activity; There is no clear quality evaluation index. [0003] This study found that both leeches and earthworms contain acetic acid, propionic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/04G01N30/68G01N30/86
CPCG01N30/02G01N30/04G01N30/06G01N30/30G01N30/68G01N30/8634G01N2030/045
Inventor 李灵云李振国王跃飞杜宇生倪开岭郝明王丹丹何子龙贾力夫
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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