Preparation method of quinolone medicine passing type solid-phase extraction column

Abstract

The invention relates to a preparation technology of a solid-phase extraction column, and aims to provide a preparation method of a quinolone medicine passing type solid-phase extraction column. The method comprises the following steps: mixing a spiral COFs composite material with a proper amount of pure organic solvent, and carrying out ultrasonic treatment until the materials are uniformly dispersed; filling the pretreated material into a solid-phase extraction column made of a polypropylene material, and blocking two ends by using sieve plates respectively; mixing a traditional solid-phase extraction adsorbent with a proper amount of pure organic solvent, and performing ultrasonic treatment until uniform dispersion; continuously filling the pretreated material into the solid-phase extraction column, and blocking the end parts by using sieve plates to serve as sample introduction ends; and cleaning the column with a pure organic solvent, blowing with inert gas to remove the residual organic solvent, and drying in vacuum to obtain the quinolone medicine passing type solid-phase extraction column. The method has the advantages of simple preparation process, strong food impurity interception capability, good purification effect and the like. The matrix interference effect in the mass spectrum detection process can be obviously reduced, the mass spectrum detection response signal is effectively improved, and the sensitivity is effectively improved.

Description

Technical field [0001] The present invention relates to a method of preparing a solid phase extraction column, and more particularly to a method of preparing a quinolone drug through a solid phase extraction column. Background technique [0002] Quinolone drugs are used as a class of broad-spectrive antibiotics to prevent and treat infectious diseases, and use a lot in animal husbandry. However, trace quinolone drugs remaining in poultry, water and egg products, potentially promoting the formation of drug-resistant bacteria or even super tolerance, and thereby bringing harm to human endocrine systems. In view of this, the rapid determination of quinolone drug residues in food has become the weight of the monitoring of food chemical pollutants. [0003] Since the quinolone drug residual sample matrix is ​​complex, the type is much more, the content is low (PG), so the enrichment of the sample has become the weight of the monitoring of food chemical pollutants. Currently, sample pr...

AI Technical Summary

Problems solved by technology

However, traditional solid-phase extraction cartridges mostly use C 18 , ion exchange resin, aluminum oxide and other particles are used as fillers, the simultaneous separation and purification effect of various quinolone drug residues is poor, the matrix effect is relatively strong, and the recovery rate is generally 70% or even lower

use C 18 The small column can only remove part of the non-polar lipid substances, and most of the vegetables and fruits are water-soluble polar substances, so it is difficult to achieve separation and purification

[0004] Therefore, the development of a new type of solid-phase extraction column and its application to the one-step purification of quinolone drug residues in food samples has broad application prospects and is somewhat challenging.

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