62 results about "High-performance Liquid Chromatography-UV" patented technology

The invention relates to an antibiotic detection method, and in particular, relates to a method for simultaneously detecting various antibiotics in livestock and poultry manure by high performance liquid chromatography; the method comprises the steps: pretreating a livestock and poultry manure sample, to obtain a to-be-detected liquid; performing analyte separation of the to-be-detected liquid by a high performance liquid chromatograph, and calculating peak areas corresponding to separated residual antibiotics; and quantitatively calculating according to a standard curve, to obtain the residual content. The method is applied in detection of 11 kinds of residual antibiotics in the livestock and poultry manure; compared with a high performance liquid chromatography-mass spectrometry coupled technique, the method has the advantages of lower detection cost and high detection efficiency, can simultaneously separate 11 kinds of antibiotics, has analytes without interference of major interferents, and basically does not affect qualitative and quantitative analysis of the analytes.

The invention relates to a high-performance liquid chromatography method for measuring nucleotide in milk powder, and belongs to the technical field of detection for food additives. The method comprises the following steps of: preparing a contrast solution by using a nucleotide standard substance; weighing a test sample, dissolving the test sample in distilled water through ultrasonic oscillation, so that the nucleotide in the milk powder is dissolved in the distilled water; adjusting the pH value of the test sample solution by hydrogen nitrate until the pH value is 3 to 4, precipitating proteins, filtering and removing the proteins, fixing the volume of a filtrate by using water to obtain a to-be-tested solution; measuring nucleotide chromatographic peak areas in the test solution and the contrast solution by high performance liquid chromatography; and calculating the nucleotide content in the test sample by an external standard method according to the concentration of the nucleotidein the contrast solution, the nucleotide chromatographic peak area in the test solution and the mass of the test sample. The method is simple, convenient to operate and high in precision; proteins can be completely separated, so that interference with the measurement for the nucleotide is eliminated; and a good condition is supplied to measurement of the nucleotide in the test sample.

The invention discloses a method for detecting an initial material II in apixaban through reversed-phase high performance liquid chromatography. The method comprises the following steps: 1, preparing a sample solution; 2, detecting the sample through reversed-phase high performance liquid chromatography; and 3, calculating the content of every impurity and the content of total impurities in the sample through an area normalization technique. The method for detecting initial material II related substances in apixaban through reversed-phase high performance liquid chromatography realizes complete separation of the chromatographic peaks of the initial material II and all the impurities through a simple mobile phase component; and the detection result of the method is accurate and reliable, so the method makes control of the quality of the initial material in the apixaban synthesis process possible.

The invention discloses a method used for detecting caffeic acid content in cigarette sidestream smoke by high performance liquid chromatography-tandem mass spectrum. The method comprises following steps: (1) total particulate matters in smoke are collected by a fishtail cover and a filter disc; (2) the filter disc is placed in a methanol solution containing internal standard substances for extraction so as to obtain an extract; (3) the extract is filtered, and then is detected by a high performance liquid chromatography-tandem mass spectrometer; and (4) the content of caffeic acid is quantitatively analyzed by internal standard method. According to the method used for detecting caffeic acid content in cigarette sidestream smoke by high performance liquid chromatography-tandem mass spectrum, pre-treatment is simple, separating effect is excellent, qualitative analysis is accurate, sample analysis time is short, and detection sensitivity is high.

The invention belongs to the technical field of food detection methods and particularly relates to a method for testing food additives in foods by virtue of a high performance liquid chromatography. The method comprises the followings steps: preparing standard series working solutions; preparing to-be-tested liquid; and determining: testing the standard series working solutions and the to-be-tested liquid by virtue of a high performance liquid chromatograph with a DAD detector, carrying out gradient elution, and carrying out detection under a variable wavelength, wherein a flow phase of the high performance liquid chromatograph is gradient-mixed liquid of an ammonium acetate solution and methanol, and an acetic acid solution is added into the ammonium acetate solution. By improving a sample processing method, five additives can be synchronously extracted, and the detection efficiency is improved; by optimizing the ratio and analysis parameters of the flow phase of the high performance liquid chromatography, chromatographic peaks of the five additives are well separated, and the accurate quantitative analysis is facilitated; and by detecting the area of the chromatographic peak corresponding to the characteristic absorption wavelength of each additive in a retention time, a relatively low detection limit can be obtained, and the interference of other components can be well eliminated.

The invention provides a method for analyzing related substances of terbutaline sulfate via high performance liquid chromatography. A C18 reversed chromatographic column is used, a mobile phase is a mixture of a 0.05 M ammonium acetate buffer solution with pH 4.0 and methanol, gradient elution is carried out, and qualitative and quantitative detection of terbutaline and 9 impurities is achieved byonce sample injection. Compared with the prior art, the method provided by the invention has the advantages of being able to not only detect more impurities, but also having good sensitivity to various impurities. In the specific application, according to the detection result of each batch of samples, the limit of the key impurity formulated, and the economic applicability in the actual production process is greatly improved.

The invention discloses a high performance liquid chromatography detection method for the abamectin content in edible vegetable oil. The method comprises the steps that a sample is subjected to vortex oscillation extraction through methanol, purified with an ODS C18 solid-phase extraction column, subjected to derivatization through N-methylimidazole and trifluoroacetic anhydride and lastly determined through a liquid chromatography-fluorescence method. According to the method, few reagents are adopted, and the cost is reduced; a sample pretreatment method is simple, the purification effect is good, maintenance of instruments in the using process is reduced, and the technical problems that when a liquid chromatography-ultraviolet detector is used, the sensitivity is too low, and matrix interference is serious are solved; a determined result is accurate and good in repeatability, the detection specificity and sensitivity are high, and a reference is provided for detection of the abamectin content in the edible vegetable oil.

The invention relates to the field of medicament analysis, and discloses an ultra-high performance liquid chromatography method for determining the content of rheum lhasaense. The method comprises the steps of: by taking acetonitrile-0.1% phosphoric acid water solution as a mobile phase, gradiently eluting rheum lhasaense-carried sample solution to enter an ultra-high performance liquid chromatograph; carrying out gradient elution by taking the acetonitrile-0.1% phosphoric acid water solution as a mobile phase; setting a standard sample, determining the response values of deoxyrhaponticin peak, triptophenolide peak, and polydatin peak in the rheum lhasaense sample by using an ultraviolet detector so as to respectively correspond to sample sizes, and calculating the content of ingredients in the rheum lhasaense sample through comparing the size of the response values with a standard sample. According to the method, the analysis and detection time of the sample can be shortened, the method has the characteristics of being good in separation effect, accurate to determine, high in sensitivity, strong in specificity, simple and convenient for an analysis method and the like, is also capable of saving the usage amount of a solvent, thus having great application prospects.

The invention discloses a method for quantitative determination of prostaglandin in a biological sample. An ultra performance liquid chromatography-differential mobility spectrometry-tandem mass spectrometry (UPLC-DMS-MS/MS) detection system is employed to determine prostaglandin in the biological sample, and a DMS pool is added between the high performance liquid chromatogram and the mass spectrum. According to the invention, the DMS pool is mounted between an ion source of the mass spectrum and a first quadrupole, the interference of limaprost in blood plasma is further removed by DMS, thusreducing the liquid chromatogram separation difficulty, changing two-dimensional chromatogram to one-dimensional chromatogram, greatly shortening the sample analysis time, and improving the sample analysis flux.

Belonging to the technical field of food safety detection, the invention discloses an ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry screening method for a nutrition enhancer in milk and dairy product. The method includes: utilizing optimized QuEChERS method to perform pretreatment on a sample; using ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry for screening, adopting full scanning/data dependent acquisition, full scanning/variable data non-dependent acquisition, and full scanning/all-ion fragmentation acquisition scanning modes, selecting positive ion or negative ion mode scanning to obtain complete first-order and second-order spectrum; applying Exact Finder 2.5 software to process the obtained first-order and second-order spectrum, and extracting compound information; and comparing the information in the established spectrum library with the screening result of the detected object, and confirming the composition of the nutrition enhancer in the milk and dairy product. The mass spectrometer used in the invention has the main advantages of high resolution, accurate qualitative and quantitative result, high sensitivity and high quality precision, etc., and can be applied to analysis of complex matrices.

The invention discloses a rapid detection analysis method for statin drugs in a water body, and belongs to the trace detection field of organic pollutants in the water body. In the method for detecting statin substances of lipid-lowering drugs, a solid-phase extraction small column is combined with a high performance liquid chromatography-mass spectrum method, and various statin drugs in the waterbody can be detected efficiently and quickly simultaneously. The method comprises the following steps: filtering a water sample, and adding an internal standard substance; then using the solid-phaseextraction small column to extract and purify the statin substances in the sample; and then detecting the content of a target object in the water sample through a liquid chromatogram-mass spectrometer. The method is simple in treatment steps for the water sample, convenient in operation and high in stability, and the sample applicable for the liquid chromatograph/mass spectrometer can be obtainedquickly; the pretreatment cost of the sample is low, rapid and efficient detection can be carried out, and the method is high in detection speed, high in degree of automation, sensitive in response and convenient for industrial application, is a simple, convenient, rapid and accurate qualitative and quantitative detection method, and is applicable for popularization and application.

The invention discloses a method for detecting 2,2,6,6-tetramethylpiperidinooxy with high performance liquid chromatography-mass spectrometry. The method comprises the steps that 1, a test solution and a reference stock solution is prepared; 2, the test solution and the reference stock solution with a certain concentration gradient are subjected to sample introduction, a high performance liquid chromatograph mass spectrometer is used for detecting and recording a chromatogram; and 3, linear regression analysis is performed on the mass concentration of the reference stock solution and chromatogram peak area, a regression equation and correlation coefficients are obtained, and a standard curve is manufactured; and the peak area of 2,2,6,6-tetramethylpiperidinooxy in the chromatogram of the test solution is utilized to calculate the content of 2,2,6,6-tetramethylpiperidinooxy by an external standard method. The method provided by the invention is the first method for detecting 2,2,6,6-tetramethylpiperidinooxy developed in the field. The method has accurate detection result, high sensitivity, good stability, and low detection limit, and totally meets detection requirements for genotoxic impurities in the field.

The invention discloses a high-performance liquid chromatography-mass spectroscopy of iprodione in tobacco and tobacco products. The high-performance liquid chromatography-mass spectroscopy is characterized by comprising the following steps: completely decomposing iprodione in a sample as metabolite [iprodione-des-(N-isopropyl formamide)], acidizing the metabolite by using hydrochloric acid, performing liquid-liquid extraction by using dichloromethane, filtering, nitrogen-blowing to proximate dry, re-dissolving by using acetonitrile, measuring the iprodione metabolite content in the sample byusing chromatography-mass spectroscopy, thereby directly measuring the content of iprodione in the sample. Through the method disclosed by the invention, the disadvantage of a sample treating method in the prior art is overcome, the blank of the substance measurement is filled, thereby providing various references for the measurement of related residual limit and the development of the method technology; the sample pretreatment method and an instrument detection method are optimized for the tobacco sample, and the method disclosed by the invention has the following effects in comparison with the prior art: the sample pretreatment process of the method is simple and fast, and the method has the advantages of being accurate in operation, high in sensitivity and good in repeatability.

The invention relates to a method for detecting the content of a sulphobetaine surfactant in an oil well injection-production fluid through high performance liquid chromatography. The method comprisespreparing a standard substance solution and a sample solution from a blank oil well injection-production fluid, feeding 20 micro-liter of the sample, and calculating the content through an external standard method, wherein the high performance liquid chromatography conditions comprise a C18 chromatographic column as a chromatographic column, an evaporative light scattering detector as a detector,methanol and water as mobile phases, use of gradient elution with 70% methanol in 0-2min and 100% methanol after 2.01min, airflow velocity of 2-2.5L/min and an evaporating temperature of 110-120 DEGC. The method has the advantages of fast analysis speed, simple operation, safety, reliability, quantification of the content of a sulphobetaine surfactant based on an external standard method, few interference factors, high accuracy, good repeatability and high sensitivity.

The invention relates to a method for measuring Farnesal contents in medicine loading micelle by high performance liquid chromatography, and belongs to the field of medicine analysis. An Agilent-1260high performance liquid chromatograph and an ultraviolet detector are adopted, a detection wavelength is 216nm, and a chromatographic condition is that a chromatographic column is a reverse chromatographic column which selects octadecylsilane chemically bonded silica as a filler; a moving phase is preferably mixture of acetonitrile and water, wherein the volume ratio of acetonitrile to water is 80:20; and a flow rate is 0.8-1.2mL/min, a sample introduction amount is 5-15muL, and a column temperature is 20-40DEG C. The method has the characteristics of being simple in operation, high in specificity, high in accuracy, good in precision and the like, and an intra-day and daytime standard deviation does not exceed 1.2%. Therefore, by use of the method, the requirement that Farnesal contents can be accurately and effectively measured can be met, and the method can be accurately used for testing Farnesal contents in experiments, including the encapsulation efficiency, the medicine loading capacity, the in vitro release and the like of the Farnesal-loaded medicine loading micelle.

The invention discloses a method adopting an ultra-high performance liquid chromatography-quadrupole static electric field track ion trap mass spectrum for screening a corrosion remover in milk and dairy products, and belongs to the technical field of food safety testing. An optimized QuEChERS method is adopted for preprocessing a sample; the ultra-high performance liquid chromatography-quadrupole static electric field track ion trap mass spectrum is applied for performing screening, full-scanning/data dependency collection, full-scanning/variable data independent collection and a full-scanning/full-ion fragmentation collection scanning mode are adopted, positive ion or negative ion mode scanning is selected, and complete first-order or second-order spectra are obtained; Exact Finder 2.5 software is applied for processing the obtained first-order or second-order spectra, and compound information is extracted. Information in a building spectrum library and a tested substance screening result are compared, and the composition of the corrosion remover in milk and dairy products is ensured. An adopted mass spectrometer has the main advantages of being high in resolution, accurate in qualitative and quantitative result, high in sensitivity, high in quality precision and the like and can be applied to complex matrix analysis.