Ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry screening method for nutrition enhancer in milk and dairy product

A nutrient fortifier and ultra-high performance liquid phase technology, which is applied in material separation, measuring devices, instruments, etc., can solve the problems of high cost of materials and equipment, complicated pretreatment operations, and low high-throughput, and achieve accurate qualitative and quantitative results. , Fast positive and negative switching scanning mode, the effect of high quantitative ability

Inactive Publication Date: 2017-03-22
SHAANXI UNIV OF SCI & TECH
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Problems solved by technology

However, at present, there are few high-throughput and rapid detection methods for exogenous risk substances in milk and dairy products. The pre-treatment

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  • Ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry screening method for nutrition enhancer in milk and dairy product
  • Ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry screening method for nutrition enhancer in milk and dairy product
  • Ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry screening method for nutrition enhancer in milk and dairy product

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Embodiment Construction

[0034] The invention provides an ultra-high performance liquid chromatography-quadrupole electrostatic field orbital ion trap mass spectrometry screening method for nutritional fortifiers in milk and dairy products, which is characterized in that the equipment used in the method includes ultra-high performance liquid chromatography - Quadrupole electrostatic field orbital ion trap mass spectrometer. Include the following steps:

[0035] First, prepare matrix solution and standard solution; use the optimized QuEChERS method to extract and clean up milk and dairy product samples;

[0036] Secondly, ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry (Q-Orbitrap) was used for screening, using electrospray ionization source (ESI) and full scan / data dependent acquisition (Full MS / dd-MS 2), full scan / variable data independent acquisition (Full MS / vDIA), full scan / full ion fragmentation acquisition (Full MS / AIF) three scan mod...

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Abstract

Belonging to the technical field of food safety detection, the invention discloses an ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry screening method for a nutrition enhancer in milk and dairy product. The method includes: utilizing optimized QuEChERS method to perform pretreatment on a sample; using ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry for screening, adopting full scanning/data dependent acquisition, full scanning/variable data non-dependent acquisition, and full scanning/all-ion fragmentation acquisition scanning modes, selecting positive ion or negative ion mode scanning to obtain complete first-order and second-order spectrum; applying Exact Finder 2.5 software to process the obtained first-order and second-order spectrum, and extracting compound information; and comparing the information in the established spectrum library with the screening result of the detected object, and confirming the composition of the nutrition enhancer in the milk and dairy product. The mass spectrometer used in the invention has the main advantages of high resolution, accurate qualitative and quantitative result, high sensitivity and high quality precision, etc., and can be applied to analysis of complex matrices.

Description

[0001] 【Technical field】 [0002] The invention belongs to the technical field of food safety detection, and in particular relates to an ultra-high performance liquid chromatography-quadrupole electrostatic field orbital ion trap mass spectrometry screening method for nutrition enhancers in milk and dairy products. [0003] 【Background technique】 [0004] Nutritional fortifiers refer to natural or synthetic substances belonging to the scope of natural nutrients that are added to foods to enhance nutritional components. Foods that have been fortified are called fortified foods. The nutritional fortifiers allowed to be used in my country include the types stipulated in GB14880 and GB2760. Nutritional enhancers are mainly divided into vitamins, unsaturated fatty acids, nucleosides and nucleotides. Adding a certain amount of nutritional fortifiers to milk and dairy products can balance the nutrients and make up for the lack of nutrients. In recent years, people have paid more and...

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Application Information

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IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 贾玮张焱茜
Owner SHAANXI UNIV OF SCI & TECH
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