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High performance liquid chromatography-based method used for detection of eugenol in cigarette main stream smoke

A cigarette mainstream smoke, high performance liquid chromatography technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of inability to analyze the release of eugenol, and achieve the effects of avoiding interference, high sensitivity, and rapid method

Active Publication Date: 2013-10-02
CHINA TOBACCO JIANGSU INDAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for most flue-cured cigarettes, the content of eugenol is at a trace level. For such a complex background system as cigarette smoke, this method cannot accurately analyze the release of eugenol in mainstream smoke. There are certain limitations in the qualitative confirmation and accurate quantification of eugenol content

Method used

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  • High performance liquid chromatography-based method used for detection of eugenol in cigarette main stream smoke
  • High performance liquid chromatography-based method used for detection of eugenol in cigarette main stream smoke
  • High performance liquid chromatography-based method used for detection of eugenol in cigarette main stream smoke

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Embodiment 1

[0027] A method for determining eugenol in cigarette mainstream smoke based on high performance liquid chromatography, the steps are as follows:

[0028] (1) Preparation of standard working solution

[0029] Accurately weigh 0.1113g of the standard substance eugenol, first disperse and dissolve it with 2mL of methanol, and then use isopropanol to make up the volume to a 100mL volumetric flask to obtain a primary standard stock solution with a concentration of 1113mg / L; accurately pipette 10mL of a Grade standard stock solution, use isopropanol as solvent to dilute to a 100mL volumetric flask to obtain a primary standard stock solution with a concentration of 111.3mg / L; accurately pipette 50μL, 100μL, 500μL, 1mL and 5mL standard stock solution respectively , Use isopropanol as solvent to make up to 100mL volumetric flask. The concentrations of the series of standard working solutions are 0.05565mg / L, 0.1113mg / L, 0.5565mg / L, 1.113mg / L, 5.565mg / L respectively. The standard work...

Embodiment 2

[0043] The present embodiment has carried out following verification to the trapping efficiency of eugenol in the sample of the present invention:

[0044] Cigarette smoke includes two parts, particle phase and gas phase, and only the particle phase part of the smoke is captured by the Cambridge filter. In order to investigate the efficiency of the Cambridge filter in capturing eugenol in mainstream smoke, while the Cambridge filter was used to capture the particulate matter in mainstream smoke, two absorption bottles containing 20 mL were connected in series behind the Cambridge filter to absorb the mainstream smoke Eugenol in the gas phase part. After smoking 20 cigarettes, the Cambridge filter and the eugenol in the absorption liquid were analyzed, and the results are shown in Table 2. From Table 2, it can be found that the eugenol in the mainstream smoke of cigarettes mainly exists in the particle phase part, therefore, the eugenol in the mainstream smoke of cigarettes ca...

Embodiment 3

[0048] The present embodiment is as follows to the qualitative confirmation method of the present invention:

[0049] The present invention must meet the following two conditions to the qualitative confirmation method of target object eugenol: 1. contrast with the retention time of standard sample chromatogram, the retention time of sample chromatogram is the allowable error of ± 2.5%; 2. with the spectrogram of standard sample contrast, from image 3 It can be seen from the figure that the preferred excitation wavelength is 290nm, and the preferred emission wavelength is 315nm, and the spectra of the samples must be consistent.

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Abstract

The invention discloses a high performance liquid chromatography-based method used for detection of eugenol in cigarette main stream smoke. The method comprises steps such as preparation of standard working solutions and sample solutions, high performance liquid chromatography analysis, and calculation of analysis results. External standard method is used for quantification in the method; the optimized detection method is simple and accurate; the chromatographic peak of the target component and the chromatographic peaks of other components can be separated easily because of adopted chromatographic conditions; when the concentration range is 0.055 to 5.5 mg / l, linear dependence of the standard curve is relatively good, correlation coefficient R2 is 0.9996, detection limit is 0.015 ug per cigarette, average relative standard deviation is 4.69%, and adding standard recovery is 82.67 to 97.46%. It can be concluded from the characteristics above that: sensitivity, repeatability and recovery rate of the method are high, and the method is suitable for qualitative and quantitative analysis of eugenol in cigarette main stream smoke.

Description

technical field [0001] The invention belongs to the technical field of cigarette physical and chemical index detection, and in particular relates to a method for measuring eugenol in cigarette mainstream smoke based on high performance liquid chromatography. Background technique [0002] The scientific name of eugenol is 4-allyl-2-methoxyphenol, the English name is Eugenol, also known as eugenol, eugenol, syringic acid, etc. It has a strong smell of carnation musk, and is the basis for blending health and fragrance series essences. It is used in the blending of fragrances such as cosmetics, soaps, and food. In addition, eugenol has a strong bactericidal effect, can be used as a local analgesic for dental caries, and also has a local antiseptic effect. In 1985, eugenol was listed as a suspected carcinogen by IARC, the International Agency for Research on Cancer. The inhalation toxicity of this substance is 250 times that of consumption, the toxicity classification is poison...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 廖惠云朱龙杰庄亚东王珂清熊晓敏石怀彬曹毅韩开冬张媛朱莹
Owner CHINA TOBACCO JIANGSU INDAL
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