Method for testing food additives in foods by virtue of high performance liquid chromatography

A technology of high performance liquid chromatography and food additives, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of easy interference of components, inaccurate measurement results, overlapping of chromatographic peaks, etc., to eliminate interference of other components, Improve detection efficiency and reduce interference

Inactive Publication Date: 2017-09-15
广东省中鼎检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these five additives are often added to various foods at the same time. The separate detection process is not only cumbersome but also easily interferes with each component. Especially when the

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  • Method for testing food additives in foods by virtue of high performance liquid chromatography
  • Method for testing food additives in foods by virtue of high performance liquid chromatography
  • Method for testing food additives in foods by virtue of high performance liquid chromatography

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Embodiment 1

[0036] The present embodiment provides a method for testing food additives in food by high performance liquid chromatography, comprising the following steps:

[0037] 1) Preparation of standard series of working solutions: Weigh the standard substances acesulfame potassium, sodium saccharin, benzoic acid, sorbic acid, dehydroacetic acid 0.1g (accurate to 0.00001g), transfer to a 100mL volumetric flask, add 20mL methanol to dissolve, Add water to make up to the mark; add water to the above mixed standard solution and dilute step by step to obtain a mixed standard series working solution of 5 additives, the concentrations are 1.0μg / mL, 5.0μg / mL, 10.0μg / mL, 20.0μg / mL , 100.0 μg / mL.

[0038] 2) Preparation of test solution:

[0039]The first step is to take a commercially available jelly as a sample, weigh 2g of the sample (accurate to 0.0001g), add 10mL of water to dilute, heat to 70°C to dissolve it, and then transfer the dissolved sample to a 50mL stoppered centrifuge tube Ad...

Embodiment 2

[0054] The present embodiment provides a method for testing food additives in food by high performance liquid chromatography, comprising the following steps:

[0055] 1) Preparation of standard series of working solutions: Weigh the standard substances acesulfame potassium, sodium saccharin, benzoic acid, sorbic acid, dehydroacetic acid 0.1g (accurate to 0.00001g), transfer to a 100mL volumetric flask, add 20mL methanol to dissolve, Add water to make up to the mark; add water to the obtained mixed standard solution and dilute step by step to obtain a mixed standard series working solution of 5 additives, the concentrations are 1.0μg / mL, 5.0μg / mL, 10.0μg / mL, 20.0μg / mL mL, 100.0 μg / mL.

[0056] 2) Preparation of test solution:

[0057] The first step is to take a commercially available cake as a sample, weigh 2g of the sample (accurate to 0.0001g), add it to a 50mL centrifuge tube with a stopper, add 25mL of water, vortex and mix, ultrasonically extract for 30min, and then add ...

Embodiment 3

[0068] The present embodiment provides a method for testing food additives in food by high performance liquid chromatography, comprising the following steps:

[0069] 1) Preparation of standard series of working solutions: Weigh the standard substances acesulfame potassium, sodium saccharin, benzoic acid, sorbic acid, dehydroacetic acid 0.1g (accurate to 0.00001g), transfer to a 100mL volumetric flask, add 20mL of methanol to dissolve, Add water to make up to the mark; add water to the obtained mixed standard solution and dilute step by step to obtain a mixed standard series working solution of 5 additives, the concentrations are 1.0μg / mL, 5.0μg / mL, 10.0μg / mL, 20.0μg / mL , 100.0 μg / mL.

[0070] 2) Preparation of test solution:

[0071] The first step is to take a commercially available sausage as a sample, weigh 2g of the sample (accurate to 0.0001g), add the sample to 10mL of n-hexane, then heat it in a water bath at 60°C for 5min, and add 1% of the sausage after the fat is d...

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Abstract

The invention belongs to the technical field of food detection methods and particularly relates to a method for testing food additives in foods by virtue of a high performance liquid chromatography. The method comprises the followings steps: preparing standard series working solutions; preparing to-be-tested liquid; and determining: testing the standard series working solutions and the to-be-tested liquid by virtue of a high performance liquid chromatograph with a DAD detector, carrying out gradient elution, and carrying out detection under a variable wavelength, wherein a flow phase of the high performance liquid chromatograph is gradient-mixed liquid of an ammonium acetate solution and methanol, and an acetic acid solution is added into the ammonium acetate solution. By improving a sample processing method, five additives can be synchronously extracted, and the detection efficiency is improved; by optimizing the ratio and analysis parameters of the flow phase of the high performance liquid chromatography, chromatographic peaks of the five additives are well separated, and the accurate quantitative analysis is facilitated; and by detecting the area of the chromatographic peak corresponding to the characteristic absorption wavelength of each additive in a retention time, a relatively low detection limit can be obtained, and the interference of other components can be well eliminated.

Description

technical field [0001] The invention belongs to the technical field of food detection methods, and in particular relates to a method for testing food additives in food by high performance liquid chromatography, wherein the food additives include acesulfame potassium, sodium saccharin, benzoic acid, sorbic acid and dehydroacetic acid. Background technique [0002] Acesulfame K and sodium saccharin are synthetic sweeteners, food additives that can improve the flavor of food; benzoic acid, sorbic acid, and dehydroacetic acid are synthetic preservatives that delay food spoilage by inhibiting the growth of microorganisms additives. These additives are widely used in food production due to their low cost and stable properties, but long-term excessive consumption will cause damage to the human body. GB 2760-2014 "National Food Safety Standard Food Additives Use Standards" stipulates the principles of use, scope of use and maximum usage of these additives. [0003] The existing na...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/74
CPCG01N30/02G01N30/74
Inventor 佟芳荻张炜潘进珠王洁
Owner 广东省中鼎检测技术有限公司
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