Method and application of detecting sulfonamide antibiotic by adopting high performance liquid chromatography

A high-performance liquid chromatography and sulfonamide technology, which is applied in the field of high-performance liquid chromatography to detect sulfa antibiotics, can solve the problems of high cost and cumbersome steps, and achieve the effects of high sensitivity, simple analysis method and good repeatability

Inactive Publication Date: 2018-08-17
SOUTH CHINA NORMAL UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to overcome the shortcomings and deficiencies of the above-mentioned prior art sulfonamide antibiotic detection method, which are high in cost, cumbersome in steps, and mostly stay in the field of animal food detection, the primary purpose of the present invention is to provide a method for rapidly detecting sulfa antibiotics by using high performance liquid chromatography. Methods

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  • Method and application of detecting sulfonamide antibiotic by adopting high performance liquid chromatography
  • Method and application of detecting sulfonamide antibiotic by adopting high performance liquid chromatography
  • Method and application of detecting sulfonamide antibiotic by adopting high performance liquid chromatography

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Embodiment 1

[0041] 1 material

[0042] 1.1 Instrument

[0043] Shimadzu automatic sampling HPLC system: Agilent chromatographic column, SPD-M20A UV-Vis detector, SPD-M20A diode array detector, LC-20AB binary high-pressure gradient pump, CTO-20A column thermostat and LC solution chromatography workstation.

[0044] 1.2 Reagents and samples

[0045] Acetonitrile (chromatographically pure) and anhydrous methanol (chromatographically pure) are products of Merck; 0.1% formic acid is a product of Fisher; ultrapure water is produced by Milli-Qsystem.

[0046] Standard sulfa antibiotics were purchased in Germany, including sulfadiazine, sulfamethoxazole, sulfamethoxine, sulfamethoxine, sulfamethazine, and sulfamethazine.

[0047] 1.3 Standard stock solution

[0048] Accurately weigh 10.00 mg of sulfonamide antibiotic standard and place it in a 1000 mL volumetric flask, add ultrapure water to dissolve, and dilute to the mark with ultrapure water and shake well to prepare a standard solution wi...

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Abstract

The invention belongs to the technical field of chemical substance residue detection and particularly relates to a method and application of detecting a sulfonamide antibiotic by adopting high performance liquid chromatography. By taking a high performance liquid chromatograph as a platform, the invention provides concrete chromatographic condition and sample pretreatment, qualitative and quantitative analysis methods of several sulfonamide antibiotics in a water body before and after treatment with an adsorbent. The analysis method provided by the invention is simple, convenient and rapid, high in sensitivity, and good in selectivity and repeatability and can be suitable for any water body treated with the adsorbent.

Description

technical field [0001] The invention belongs to the technical field of chemical substance residue detection, and in particular relates to a method and application for detecting sulfonamide antibiotics by using high performance liquid chromatography. Background technique [0002] Sulfonamides (SAs) are a general term for a class of drugs with p-aminobenzenesulfonamide structure. Sulfonamides inhibit bacterial proliferation by competitively inhibiting p-aminobenzoic acid in the folic acid metabolic cycle. After decades of development, it is now not only used as a human medicine, but also widely used in animal husbandry and aquaculture to treat various infectious diseases caused by bacteria and specific microorganisms. At present, the residues of sulfonamide antibiotics are often found in the detection of food antibiotic residues. Because of their broad-spectrum, stability, economy, ease of use, and the combination of antibacterial synergists, the effect can be increased by doz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 郑刘春阳月贝彭丹许宗林詹逸如陈树楷杨宇艺曾昊
Owner SOUTH CHINA NORMAL UNIVERSITY
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