HPLC (high performance liquid chromatography) method for detecting concentration of voriconazole in human plasma

A technology for voriconazole and human detection, applied in the medical field, can solve the problems of expensive equipment, unpopularization, and complicated treatment, and achieve the effects of simple operation, accurate results, and avoiding poor curative effect

Pending Publication Date: 2019-03-01
史长城
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  • Abstract
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Problems solved by technology

Considering that the sample processing of LC-MS / MS method is more complicated, the equipment is expensive, and the application in domestic hospitals is not popular

Method used

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  • HPLC (high performance liquid chromatography) method for detecting concentration of voriconazole in human plasma
  • HPLC (high performance liquid chromatography) method for detecting concentration of voriconazole in human plasma

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Embodiment Construction

[0017] The preferred implementation of the HPLC method for detecting the concentration of voriconazole in human plasma according to the present invention will be described in detail below in conjunction with the accompanying drawings.

[0018] figure 1 The specific embodiment of the HPLC method that shows the concentration of voriconazole in this detection human plasma:

[0019] The instruments used in the HPLC method for the detection of voriconazole concentration in human plasma include: Agilent1260 type high performance liquid chromatography, including G1311B quaternary pump, G1329B autosampler, G1316A column thermostat and G1315D DAD detector; ML204 electronic balance [Mei Teller-Toledo Instruments (Shanghai) Co., Ltd.]; MX-S vortex mixer (SCILOGEX, USA); DM0412S low-speed centrifuge (SCILOGEX, USA); 5418R low-temperature high-speed centrifuge (Eppendorf, Germany).

[0020] The reagents used in the HPLC method of voriconazole concentration in this detection human plasma i...

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Abstract

The invention discloses an HPLC (high performance liquid chromatography) method for detecting concentration of voriconazole in human plasma and relates to the technical field of medical treatment. TheHPLC method is established for detecting the concentration of voriconazole in the human plasma, which aims to provide reference for clinical rational drug use. The method comprises steps as follows:determination of chromatographic conditions, preparation of solutions, treatment of plasma samples, retention of voriconazole and internal standard chlorzoxazone, taking of an appropriate quantity ofa voriconazole stock solution, preparation of voriconazole, preparation of voriconazole, and taking of quality control plasma samples with low, medium and high mass concentrations. The method is simple to operate, has an accurate result, is suitable for detecting voriconazole in human plasma, and can assist in avoiding poor curative effect or increased adverse reactions caused by too low or too high plasma concentration. An experimental result of the HPLC method for detecting the concentration of voriconazole in the human plasma meets the measuring requirement of biological samples and is suitable for concentration monitoring of clinical treatment and pharmacokinetic study.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to an HPLC method for detecting voriconazole concentration in human blood plasma. Background technique [0002] Voriconazole is a broad-spectrum triazole antifungal drug, commonly used clinically to treat invasive fungal diseases such as invasive pulmonary aspergillosis and candidemia. The pharmacokinetics of the drug presents nonlinear characteristics. Factors such as patient age, body weight, liver function, genetic polymorphism, and drug concomitant medications will all affect its in vivo process, resulting in large inter-individual and intra-individual differences in blood drug concentration. The blood concentration of voriconazole is closely related to the clinical efficacy and safety, and too low or too high blood concentration may lead to poor curative effect or increased adverse reactions. At present, the determination methods reported at home and abroad mainly include HPL...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 史长城
Owner 史长城
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