A method for simultaneous quantitative detection of main components of Shuangshen Pingfei Granules in plasma

A quantitative detection and plasma technology, which is applied in the field of medicine and Chinese patent medicine in vivo metabolism research, can solve the problems that have not been reported, and achieve good sensitivity and accurate and reliable results.

Active Publication Date: 2021-12-14
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the pharmacokinetics of these nine components in rat plasma after oral administration of Shuangshen Pingfei Granules

Method used

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  • A method for simultaneous quantitative detection of main components of Shuangshen Pingfei Granules in plasma
  • A method for simultaneous quantitative detection of main components of Shuangshen Pingfei Granules in plasma
  • A method for simultaneous quantitative detection of main components of Shuangshen Pingfei Granules in plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1: Establishment of a method for determining the content of main components in rat plasma after oral administration of Shuangshen Pingfei Granules

[0077] Include the following steps:

[0078]Administration and collection of plasma samples

[0079] The rats were fed adaptively for one week, fasted for 12 hours before the experiment, had free access to water, and ate uniformly after 8 hours of administration. The next day, the experimental animals were randomly divided into 2 groups, 10 in each group, administered with Shuangshen Pingfei Granules by intragastric administration respectively at doses of 5 g / kg and 15 g / kg, respectively at 5, 15, 30 min and 1, 1.5, 2, 4, 8, 12, and 24 hours, 0.3 mL of blood was collected from the venous plexus behind the eyes. The collected blood samples were collected in EP tubes filled with sodium heparin, incubated in a 37°C water bath for 30 minutes, centrifuged at 5000rmp for 10 minutes, separated from the plasma, and stored...

Embodiment 2

[0121] Pharmacokinetics of main components in rat plasma after oral administration of Shuangshen Pingfei Granules

[0122] 1. Administration and collection of plasma samples

[0123] The rats were fed adaptively for one week, fasted for 12 hours before the experiment, had free access to water, and ate uniformly after 8 hours of administration. The next day, the experimental animals were randomly divided into 2 groups, 10 in each group, and the above-mentioned Shuangshen Pingfei Granules were administered by intragastric administration at doses of 5g / kg and 15g / kg respectively. After administration, 5, 15, 30min and 1 , 1.5, 2, 4, 8, 12, and 24 hours, 0.3 mL of blood was collected from the venous plexus behind the eyes. The collected blood samples were collected in EP tubes filled with sodium heparin, incubated in a 37°C water bath for 30 minutes, centrifuged at 5000rmp for 10 minutes, separated from the plasma, and stored in a -80°C refrigerator for testing.

[0124] 3.2 Pre...

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Abstract

The invention discloses a method for simultaneously quantitatively detecting the main components of Shuangshen Pingfei Granules in plasma. Contents of mangiferin, tanshinone IIA, ginsenoside Rg1, salvianolic acid B, naringin, liquiritin, cryptotanshinone, timosaponin BII and glycyrrhizic acid in rat plasma. The method includes preparation of standard stock solution, preparation of internal standard working solution, preparation of standard curve, plasma sample processing and content determination. The method provided by the invention has high sensitivity, strong specificity, good reproducibility, simple and fast operation, meets the requirements for measuring biological samples in vivo, and can be applied to pharmacokinetic research of Shuangshen Pingfei Granules after oral administration.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the technical field of in vivo metabolism research of Chinese patent medicines, in particular to a method capable of rapidly and simultaneously quantitatively detecting the main components of Shuangshen Pingfei Granules in plasma. Background technique [0002] Shuangshen Pingfei Prescription is a classic addition and subtraction prescription preferred by Professor Fan Xinsheng of Nanjing University of Traditional Chinese Medicine. The granules with the functions of clearing the lungs and moistening dryness, antitussive and anti-vomiting are used to treat pulmonary fibrosis caused by deficiency of qi and yin, obstruction of phlegm and blood stasis, and the clinical curative effect is remarkable. [0003] Studies have found that the main components of Shuangshen Pingfei Granules are saponins, phenolic acids, tanshinones, flavonoids and other compounds. Ginsenoside Rg1, tanshinone IIA, cryp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/04G01N30/72G01N30/86G01N30/88
CPCG01N30/02G01N30/06G01N30/04G01N30/72G01N30/8634G01N30/88G01N2030/062G01N2030/045G01N2030/042G01N2030/8822
Inventor 范欣生陈叶青段金廒彭国平朱振华
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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