Chemical component identification and active component screening method of Shenxiong glucose injection

A Shenxiong glucose and chemical composition technology, which is applied in the field of chemical composition identification and active ingredient screening of Shenxiong glucose injection, can solve the problems of unclear effective material basis and single composition, and achieve the effect of improving the quality control level

Active Publication Date: 2015-11-11
GUIZHOU JINGFENG INJECTION
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the problem that the effective substance basis of "Shenxiong Glucose Injection" is not yet clear, and the current quality standard has a single content determination component, the present invention conducts systematic and overall research on the chemical components of Shenxiong Glucose Injection for the first time, and the established method can quickly 、Effectively determine the chemical structure and main active ingredients of each component in Shenxiong Glucose Injection, which can provide a scientific basis for the quality evaluation of Shenxiong Glucose Injection and improve the quality control level of preparations

Method used

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  • Chemical component identification and active component screening method of Shenxiong glucose injection
  • Chemical component identification and active component screening method of Shenxiong glucose injection
  • Chemical component identification and active component screening method of Shenxiong glucose injection

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Example 1: UPLC-DAD-Q-TOF / MS Analysis and Identification of Chemical Components of Shenxiong Glucose Injection

[0046] 1. Preparation of sample solution

[0047] (1) Preparation of reference substance solution

[0048] Accurately weigh appropriate amounts of danshensu sodium, ligustrazine hydrochloride, protocatechualdehyde, caffeic acid, rosmarinic acid, salvianolic acid B, salvianolic acid A, and salvianolic acid D, and place them in eight 10mL volumetric flasks , add methanol to dissolve and dilute to the scale, shake well, and the mass concentrations are 1.056, 1.049, 1.084, 1.103, 1.020, 1.046, 1.036, 1.057 mg·mL -1 of the reference solution.

[0049] (2) preparation of need testing solution

[0050] Take 10 mL of Shenxiong Glucose Injection, dilute with water, dilute to 25 mL, and shake well.

[0051] 2. Analysis conditions

[0052] (1) Ultra High Pressure Liquid Chromatography (UHPLC) Liquid Conditions

[0053] Column: AgilentPlusPlusC 18 (2.1mm×100mm, 1....

Embodiment 2

[0068] Example 2: Cardiomyocyte Extraction and Screening of Cardiovascular Active Components in Shenxiong Glucose Injection

[0069] 1. Preparation of the test solution

[0070]Get Shenxiong Glucose Injection 100mL, concentrate under reduced pressure to a small volume, adjust the pH value of the test solution to 3 with 1mol / L hydrochloric acid solution, and pass through the OasisHLB solid-phase extraction cartridge (activate the OasisHLB cartridge with 5mL methanol, use Wash with 2mL of water until there is no alcohol smell and load the sample) to purify, first elute with water to remove the sugar components in the test sample, then elute with methanol, collect the methanol eluate and concentrate under reduced pressure to obtain the extract, and extract the extract with water Dissolved to a concentration of 2mg / mL for cell extraction experiments and UPLC-PDA-MS / MS analysis.

[0071] 2. Myocyte extraction experiment

[0072] According to the functions and indications of Shenx...

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Abstract

The invention provides a chemical component identification and active component screening method of a Shenxiong glucose injection and belongs to the technical field of traditional Chinese preparation quality controlling. In the method, main chemical components in the Shenxiong glucose injection are subjected to comprehensive on-line analysis and identification through ultra-high performance liquid chromatography-diode array detector-in-series qudropole time-of-flight mass spectrometry (UPLC-DAD-Q / TOF) to determine the structure of the compound; and meanwhile, active components in the Shenxiong glucose injection are subjected to screening with combination of myocardial cell extraction technology with ultra-high performance liquid chromatography-electrospray ionization-triple qudropole tandem mass spectrum (UPLC-ESI-MS / MS). The method can quickly and effectively determine the chemical structures of the components in traditional Chinese medicine and the main active components of the traditional Chinese medicine, and provides scientific experiment evidence of quality evaluation of the Shenxiong glucose injection.

Description

technical field [0001] The invention belongs to the technical field of quality control of traditional Chinese medicine preparations, and in particular relates to a chemical component identification and active component screening method of Shenxiong glucose injection. Background technique [0002] "Shenxiong Glucose Injection" is a compound preparation prepared from Salvia miltiorrhiza and Ligustrazine hydrochloride using modern preparation technology. It is mainly widely used in the treatment of cardiovascular diseases in clinical practice. Modern studies have shown that a variety of chemical components in Salvia miltiorrhiza and ligustrazine hydrochloride have cardioprotective biological activity, but the compound preparation "Shenxiong glucose injection" made of the combination of salvia miltiorrhiza and ligustrazine hydrochloride has the same chemical composition as the medicine. The relationship between effects has not been reported in the literature so far. Since the e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 叶湘武郑林廖尚高黄勇王永林兰燕宇
Owner GUIZHOU JINGFENG INJECTION
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